Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,872,290 A→T 8.8% intergenic (+73/+61) PP_5144 → / ← ptsP hypothetical protein/phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,872,2900AT 8.8% 332.2 / 3.7 117intergenic (+73/+61)PP_5144/ptsPhypothetical protein/phosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base A (67/35);  new base T (2/10);  total (72/45)
Fisher's exact test for biased strand distribution p-value = 1.49e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

AGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCTGTGGATA  >  NC_002947/5872141‑5872432
                                                                                                                                                     |                                                                                                                                              
 ggggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAt                                                                                                                                               >  1:1003197/1‑150 (MQ=255)
 ggggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAt                                                                                                                                               <  2:3193721/150‑1 (MQ=255)
 ggggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAt                                                                                                                                               <  1:1558591/150‑1 (MQ=255)
  gggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                                <  1:4209693/148‑1 (MQ=255)
   ggCAAAGGCGGCTGGTTGCGCGCCGGTTCGCGCCATATGGCGGGCATTTGGGGGAAATTGCGGTGATGGACTGAGTATTGGAGGTCGTCCGTTCGGGCCACTTCGCGGGAGAACCCGCTCCTACACCGGCCTGGGTCCAACCCCAGAtgt                                                                                                                                             <  1:4782436/150‑1 (MQ=255)
   ggCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgt                                                                                                                                             <  1:4081798/150‑1 (MQ=255)
    gCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTTAAACCGCTCCTACAACGGCCAGGGTCCAACCCCAGAagtg                                                                                                                                            >  1:876285/1‑150 (MQ=255)
         ggcggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACCACGGCCGGGGTCCAACCCCAGAGGTGTGTAc                                                                                                                                       >  2:3305496/1‑150 (MQ=255)
            ggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTg                                                                                                                                    <  2:2225508/150‑1 (MQ=255)
            ggcTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGACCCCGCTCCGACAACGGCCAGGGGCCCACCCCCGAGGTGTGTACggg                                                                                                                                    >  2:237065/1‑148 (MQ=255)
                ggTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtag                                                                                                                                >  1:3777107‑M2/1‑146 (MQ=255)
                   tGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggag                                                                                                                             >  1:840776‑M2/1‑143 (MQ=255)
                    gAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagc                                                                                                                            >  1:1011750‑M2/1‑142 (MQ=255)
                     aGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcg                                                                                                                           >  2:2851986‑M2/1‑141 (MQ=255)
                      gcgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgg                                                                                                                          >  1:947912‑M2/1‑140 (MQ=255)
                      gcgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgg                                                                                                                          >  1:3130517‑M2/1‑140 (MQ=255)
                      gcgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCGGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgg                                                                                                                          <  2:3141240‑M2/150‑11 (MQ=255)
                       cgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcggg                                                                                                                         >  1:2339399‑M2/1‑139 (MQ=255)
                       cgcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcggg                                                                                                                         >  2:261293‑M2/1‑139 (MQ=255)
                        gcAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTGGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggt                                                                                                                        >  2:3253016‑M2/1‑138 (MQ=255)
                             ttCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                                 <  2:4020414‑M2/136‑4 (MQ=255)
                             ttCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                                 >  1:4020414‑M2/1‑133 (MQ=255)
                               cGGGCCTGTGGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccg                                                                                                                 >  2:5080602‑M2/1‑131 (MQ=255)
                                  gCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGGACCCCCGCCAACAACCGCCGGGGTCCAACCCCCCATGTGTGTAAGTGgtaggagcggggtgacccgcga                                                                                                              <  1:3500946‑M2/150‑23 (MQ=255)
                                   ccTTTGGGCGGGCTTTTGGGGTAAATTGAGTTGTTGGTCTGCGTATTTGATGTTGTCTGTTCGGGCCTCTCCGCGGGTGACCCCGCTCCTACAACGGCCTGGGACCAACCCCAGATGTGTGTACGTGgtaggagcgggtccacccgcgaa                                                                                                             >  2:4727054‑M2/1‑127 (MQ=255)
                                     ttttGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCACCAACAACCGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgacgc                                                                                                           <  2:3525449‑M2/150‑26 (MQ=255)
                                     ttttGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGGGTACCCCCGCCCACAACAGCCAGGGGGCAACCCCCCAAGTGGGTAAGGGGTGGGAGCGGGGTCACCCCCCAaga                                                                                                           >  1:1109522/1‑150 (MQ=255)
                                     ttttGGCGGGAATTTGGGGTAAATTGATTTGTTGGTCTGATTATTTTATTTTGTATGTTCGGGCATCTTCGCGGGTTAACCCGATCCTACAACGGCCTGGTTCAAACCCAAGATGTGTGTACGTGTTAGGAGCGGGTTCACCCGCGAaga                                                                                                           <  2:3833784/150‑1 (MQ=255)
                                        tGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtagga                                                                                                                              <  1:3867882‑M2/128‑7 (MQ=255)
                                                   tGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAAACCCCTCCAACAACGGCCTGGGGCCAACCCCACATGTGTGTACGTGGTAGGAGCGGGTTCACCCCCGAAGAGGCCGGCACaaaaa                                                                                             >  2:3366792/1‑148 (MQ=255)
                                                   ggggggAATTTGTGTTGTTGGTCTGAGTATTTGTTGTGGTCGGTTCGGGCCTCTGCGGGGGTGACCCCGCTCCAACACGGGCCTGGGTCCAACCCCAGTTGGGTGTACGTGGTCGGCGGGGTTTCACCCGCGAAGAGGCCGGCACAAACa                                                                                             <  2:3118724/149‑1 (MQ=255)
                                                     ggggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAAAGGCCAGGGTCCAACCCCAGATGTGGGTACGGGGTAGGAGCGGGGTCACCCGCGAAGAGGCCGGCACAAAAAGc                                                                                           >  2:424810/1‑150 (MQ=38)
                                                       ggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGg                                                                                                                                   <  1:4800102‑M2/108‑2 (MQ=255)
                                                       ggAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGg                                                                                                                                   >  2:4800102‑M2/1‑107 (MQ=255)
                                                           aTTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGTACCCGCTCCAACAAAGGCCGGGGGCCAACCCCAGAAGTGTGTACGTGGTGGGTGCGGGTTCACCCGCGAAGAGGCCGGCCCAAAAAGCAACAcc                                                                                     >  2:2461540/1‑150 (MQ=255)
                                                             tGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgtgt                                                                                                                                           <  2:4578805/94‑1 (MQ=255)
                                                             tGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgtgt                                                                                                                                           >  1:4578805/1‑94 (MQ=255)
                                                              gAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAAACCCCACCAACAAAGGCCTGGGTACAACCCCAGAAGGGTGTACGTGGGAGGAGCGGGGTCACCCCCCAAGAGGCCGGCACAAACAGCAAAAcccac                                                                                  >  1:2694866/1‑148 (MQ=255)
                                                                   gttgGTCTGATTATTTGATGTTGTCTTTTCGGGCCCCTTCGCGGGTGAACCCGCTCCTCCAACGGCCTGGGTCCAACCCCAGTTGTGTTTACGTGTTAGGAGGGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAgg                                                                             <  2:4910028/150‑1 (MQ=38)
                                                                   gttgGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTAAAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCCGGTTCACCCGCCAAGAGGCCGGCACAAACACCATCACCCTCACAgg                                                                             >  2:3033941/1‑150 (MQ=255)
                                                                   gttgGACTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCCCACCAACAACCCCCAGGGTCCAACCCCAGAAGTGTGTACCAGGTAGGAGCGGGTTCACCCGCGAAGACGCCGGCACAAACAGCATCACCCTCACAgg                                                                             >  2:3528761/1‑150 (MQ=255)
                                                                       gTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                                >  2:5107778/1‑79 (MQ=255)
                                                                       gTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGa                                                                                                                                                <  1:5107778/79‑1 (MQ=255)
                                                                        tCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGAtgtgtg                                                                                                                                          <  1:3161094/84‑1 (MQ=255)
                                                                          tGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgta                                                                                                                                 >  1:5191027‑M2/1‑88 (MQ=255)
                                                                            tttttttGTTGTTGTGTGTTCGGGCCTCTTCGCGGTTGAACCCGCACCAACACCGGCGTGGGGCCAACCCCATTTTTGTGTACGTGGTAGGAGCGGTTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTttatta                                                                    <  2:4323571/146‑1 (MQ=255)
                                                                               aTTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGTTGTTGGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGcc                                                                 <  2:1313512/150‑1 (MQ=255)
                                                                                tttGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCGCCTACCACGGCCTGGGGGCAAACCCAGCTGGGTGTACGTGGGAGGAGCGGGTTCACCCGCGACGCGGGCGGCCCACACAGCAGCACCCTCACAGGGGGTTAGGAGccc                                                                >  2:4242160/1‑150 (MQ=25)
                                                                                 ttGATGATGTCTGTTCGGGCCTCTTCGCGGGTGAACCCCCACCAACAACCGCCGGGGGCCAACCCCACAAGGGGGTACCGGGGACGAGCGGGTTCACCCGCCCCCAGGCCGCCCCAAACACCAACACCCGCACCCGGGGTTAGTAGcccc                                                               >  2:4272404/1‑150 (MQ=255)
                                                                                   gATGTTGTCTGTTCGGGCCTCTTCGCGGGTTTACCCGCTCCGACAACGGCCTGGGTCCAAACCCAGATGTGTGTACGTGGTTGGGGCGGGGTCACCCGCGAAGAGGGCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGgccccg                                                             >  2:3188594/1‑148 (MQ=255)
                                                                                   gATGTTGTCTGTTCGGGCCTCTTCGCGGGTTTACCCGCACCTACAACCGCCGGGGTCCAAACCCAGCTGTGTGTAGGTGGGAGGGGCGGGTTAAACCCCCAACAGGCCGGCCCAAAAAGCATCCCCCAGACCGGGTTTTATTATCCCCAg                                                             >  2:3188723/1‑150 (MQ=255)
                                                                                         gtCTTTTCGGGCCTCGGCGGGGGTCACCCGGCGCCTAAACGGGCCTGGGTCCACCCCATTTTGTGGGTGGGTGTTCGGCGGGGGTACCCCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCaa                                                       <  2:4582245/150‑1 (MQ=255)
                                                                                         gtCTGTTCGGGCCTCTTCGCGGGTGAACCCCCACCAACAACCGCCGGGGTCCAACCCCCCAGGTGGGGACGGGGTCGGAGCGGGTTCACCCCCCAAGAGGCCCGCACAAACAGCAACAACCACCCAGGGGTTTAGTAGCCCCCCGCCCaa                                                       >  2:1882029/1‑150 (MQ=255)
                                                                                          tCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCAGGGTCCAAACCCAGTTGTGTGTACGTGGTAGGAGCGGGTTCACCCCCGAAGAGGCCGGCACAAACAGCATCACCCACACACGGTTTTATTAGCCCCAGGCCCaaa                                                      >  2:4966216/1‑149 (MQ=38)
                                                                                              ttCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                                  >  1:271423‑M2/1‑68 (MQ=255)
                                                                                              ttCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcaccc                                                                                                                  <  2:271423‑M2/86‑19 (MQ=255)
                                                                                                      tctTCGGGGGTTACCCCGATCCTCCCCCGGCCTGGGTCCAACCCCTGTTGTTGGTACGTGGTTGGTGGTGTTTCACCCGCTAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCaa                                          <  2:4297300/150‑1 (MQ=255)
                                                                                                      tctTCGCGGGTGGACCCGCGCCGACCACGGCCGGGGTCCAACCCCAGATGTGTGTACGGGgtaggagcgggttcacccgcgaagaggccggcacagacaacagcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaa                                          <  2:5074835‑M2/150‑91 (MQ=255)
                                                                                                        ttCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGCTGTGTGTACCTGGGAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAccc                                        >  2:4809765/1‑150 (MQ=255)
                                                                                                        ttCGCGGGTGAACCCGCTCCTACAAAGGCCTTGGTGCAAACCCAGTTTTGTGTACGTGGTAGGTGCGGGTTAACACGCGAAGAGGGCGGCACAAAAAGCATCACCCTCAAAGGGTTTTATTATCCCCAGGCCCAATCATCCGCGCCAAcc                                        >  2:4650364/1‑150 (MQ=255)
                                                                                                            ggggTGAACCCGCTCCTAAAACGGCCTGGGTCCAACCCCTGTTGTGTGTACTTGGTAGGTGCGGTTTCACCGGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAg                                    <  2:1726020/149‑1 (MQ=255)
                                                                                                            cgGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCTGTTGTGTGTACGTGGTAGGTGCGGTTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAg                                    <  2:1726037/150‑1 (MQ=255)
                                                                                                             gggTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAgg                                   >  1:380251/1‑150 (MQ=255)
                                                                                                             gggTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGGAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAgg                                   >  1:2430252/1‑150 (MQ=255)
                                                                                                               gTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGtt                                 >  1:4955457/1‑150 (MQ=255)
                                                                                                               gTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGtt                                 >  1:4340514/1‑150 (MQ=255)
                                                                                                               gTGAAACCGCTCCTACAACGGCCTGGGTCCAAACCCAGATGTGTGTACGTGGTAGGAGAGGGTTCACCCGCGAAGAGCCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCACCAGGCCCAATCATCCGCGCCAACCCAAGGtt                                 >  2:4516399/1‑150 (MQ=255)
                                                                                                                  aaCCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTttatt                                                                     <  1:5388260/111‑1 (MQ=255)
                                                                                                                  aaCCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTttatt                                                                     >  2:5388260/1‑111 (MQ=255)
                                                                                                                   aCCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaa                                 >  2:5303252‑M2/1‑47 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcc                                                  >  1:2230246‑M2/1‑45 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  2:3281986/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  1:2397653/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  2:1185561/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  1:3187426/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  1:1206881/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  1:5239537/1‑150 (MQ=255)
                                                                                                                     ccGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAg                           >  1:1493896/1‑150 (MQ=255)
                                                                                                                       gCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctc                         <  2:3997646‑M2/150‑108 (MQ=255)
                                                                                                                       gCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAggg                         >  2:5244207/1‑150 (MQ=255)
                                                                                                                       gCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAggg                         >  2:3428608/1‑150 (MQ=255)
                                                                                                                         tCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaaggggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctcaa                       <  2:3846939‑M2/150‑110 (MQ=255)
                                                                                                                         tCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaactcaatttcccccaaatgcccgccaaaaggcccgaaactgcgctcaa                       <  1:216544‑M2/150‑110 (MQ=255)
                                                                                                                           cTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAg                     >  1:1842810/1‑150 (MQ=255)
                                                                                                                            tACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGt                    >  1:3204375/1‑150 (MQ=255)
                                                                                                                             aCAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaa                                                                      <  2:4515172‑M2/99‑63 (MQ=255)
                                                                                                                             aCAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGgtaggagcgggttcacccgcgaagaggcccgaacagacaacatcaaatactcagaccaacaa                                                                      >  1:4515172‑M2/1‑37 (MQ=255)
                                                                                                                               aaCGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGc                 >  1:1045488/1‑150 (MQ=255)
                                                                                                                                        ggTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCtg        >  2:3990324/1‑150 (MQ=255)
                                                                                                                                         ggccACCCCCCGTTGTGGTTAGGGGGTTGGCGGGGGTCCCCCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCtgt       <  2:5003696/148‑1 (MQ=255)
                                                                                                                                         gcccAACCCCAGTTGTGTGTAGGTGGTTGGAGGGGTTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCtgt       <  2:5146204/148‑1 (MQ=255)
                                                                                                                                            cAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCTGTGGa    >  1:2861082/1‑150 (MQ=255)
                                                                                                                                             aaCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCg                                                                                                      >  2:3997104/1‑51 (MQ=255)
                                                                                                                                             aaCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAAc                                         >  2:2410253/1‑112 (MQ=255)
                                                                                                                                             aaCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCaa                                     >  2:1267536/1‑116 (MQ=255)
                                                                                                                                             aaCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCTGTGGAt   >  2:594912/1‑150 (MQ=255)
                                                                                                                                              aCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGttctt                              <  1:4333096/122‑1 (MQ=255)
                                                                                                                                              aCCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCTGTGGATa  >  2:4990794/1‑150 (MQ=255)
                                                                                                                                                     aTGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAat                                                      >  1:1316007/1‑91 (MQ=255)
                                                                                                                                                     aTGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAat                                                      <  2:1316007/91‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
AGGGGCAAAGGCGGCTGGTTGAGCGCAGTTTCGGGCCTTTTGGCGGGCATTTGGGGGAAATTGAGTTGTTGGTCTGAGTATTTGATGTTGTCTGTTCGGGCCTCTTCGCGGGTGAACCCGCTCCTACAACGGCCTGGGTCCAACCCCAGATGTGTGTACGTGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAAACAGCATCACCCTCACAGGGTTTTATTAGCCCCAGGCCCAATCATCCGCGCCAACCCAAGGTTCTTCAGGGCCAGTTGCAGCGAGCTGTGGATA  >  NC_002947/5872141‑5872432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑