Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,885,573 C→G 6.0% A60G (GCC→GGC)  PP_5157 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,885,5730CG 6.0% 725.0 / 25.3 218A60G (GCC→GGC) PP_5157hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (115/90);  new base G (11/2);  total (126/92)
Fisher's exact test for biased strand distribution p-value = 4.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 226 total aligned reads displayed.

AGTACTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGC  >  NC_002947/5885424‑5885722
                                                                                                                                                     |                                                                                                                                                     
aGTACTGATCAGTCCATTGTCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGc                                                                                                                                                       <  1:3733346/150‑1 (MQ=255)
    cTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTg                                                                                                                                                   >  1:1498032/1‑150 (MQ=255)
    cTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTg                                                                                                                                                   >  2:1101070/1‑150 (MQ=255)
     tGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTgg                                                                                                                                                  >  1:2404367/1‑150 (MQ=255)
      gATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGc                                                                                                                                                 <  1:237430/150‑1 (MQ=255)
      gATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGc                                                                                                                                                 <  2:122954/150‑1 (MQ=255)
       aTCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGcc                                                                                                                                                >  2:516477/1‑150 (MQ=255)
        tCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCa                                                                                                                                               >  1:5296976/1‑150 (MQ=255)
          aGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAg                                                                                                                                             <  2:3445226/150‑1 (MQ=255)
            tCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGa                                                                                                                                           >  1:31317/1‑150 (MQ=255)
            tCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGa                                                                                                                                           >  2:1504948/1‑150 (MQ=255)
             ccATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGaa                                                                                                                                          <  2:2392516/150‑1 (MQ=255)
               aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACt                                                                                                                                        >  1:2005033/1‑150 (MQ=255)
               aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACt                                                                                                                                        >  1:2786060/1‑150 (MQ=255)
               aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACt                                                                                                                                        >  1:836864/1‑150 (MQ=255)
               aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACt                                                                                                                                        <  2:3714977/150‑1 (MQ=255)
                   gCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGAt                                                                                                                                    >  2:2956111/1‑150 (MQ=255)
                     tCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTgg                                                                                                                                      <  1:4355897/146‑1 (MQ=255)
                     tCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGAttt                                                                                                                                  <  1:1669677/150‑1 (MQ=255)
                     tCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGAttt                                                                                                                                  >  2:2138071/1‑150 (MQ=255)
                       aGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgc                                                                                                                                <  2:2428031/150‑1 (MQ=255)
                       aGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgc                                                                                                                                <  1:3403975/150‑1 (MQ=255)
                          cGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgcgcg                                                                                                                             >  1:1355232/1‑150 (MQ=255)
                           ggAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgcgcgc                                                                                                                            <  2:1665565/150‑1 (MQ=255)
                            gAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGcc                                                                                                                           >  2:1919912/1‑150 (MQ=255)
                                tGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGTCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCATGGAACTGGATTTGCGCGCCGAGt                                                                                                                       <  2:749679/150‑1 (MQ=255)
                                tGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGt                                                                                                                       <  1:1048255/150‑1 (MQ=255)
                                     gacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtc                                                                                                                  <  2:1977733/150‑1 (MQ=255)
                                     gacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtc                                                                                                                  >  2:4203049/1‑150 (MQ=255)
                                     gacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtc                                                                                                                  <  2:1572766/150‑1 (MQ=255)
                                     gacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtc                                                                                                                  >  1:5344778/1‑150 (MQ=255)
                                        gaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                               >  2:388160/1‑150 (MQ=255)
                                            tCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGccc                                                                                                           <  2:1043570/150‑1 (MQ=255)
                                            tCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGccc                                                                                                           <  2:3903022/150‑1 (MQ=255)
                                              gCCGACCGGGGCGAACTGCGCATTGCCGTACAGACCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCg                                                                                                         <  2:4743575/150‑1 (MQ=255)
                                               ccGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCgg                                                                                                        >  2:4835331/1‑150 (MQ=255)
                                                       ggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGaa                                                                                                <  2:1301015/150‑1 (MQ=255)
                                                       ggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGaa                                                                                                <  2:1771354/150‑1 (MQ=255)
                                                                   aTTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgt                                                                                    <  2:3737837/150‑1 (MQ=255)
                                                                        cGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               <  1:1919912/150‑1 (MQ=255)
                                                                          tACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                             <  2:2224651/150‑1 (MQ=255)
                                                                          tACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                             <  2:3682623/150‑1 (MQ=255)
                                                                          tACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                             <  2:1214282/150‑1 (MQ=255)
                                                                            cAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGGCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGt                                                                           <  2:2021162/150‑1 (MQ=255)
                                                                             aGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTg                                                                          <  2:312529/150‑1 (MQ=255)
                                                                                ccGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCa                                                                       >  2:3284387/1‑150 (MQ=255)
                                                                                 cGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                      <  1:988651/150‑1 (MQ=255)
                                                                                 cGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                      <  2:1064869/150‑1 (MQ=255)
                                                                                 cGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                      <  1:2277739/150‑1 (MQ=255)
                                                                                     aaCTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTAc                                                                  <  1:516477/150‑1 (MQ=255)
                                                                                        tCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGAc                                                               >  2:3233153/1‑150 (MQ=255)
                                                                                        tCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGAc                                                               >  1:4123351/1‑150 (MQ=255)
                                                                                          gCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACAt                                                             >  1:661863/1‑150 (MQ=255)
                                                                                          gCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACAt                                                             >  2:5281906/1‑150 (MQ=255)
                                                                                           ccATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATc                                                            >  1:2720957/1‑150 (MQ=255)
                                                                                                cGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAtt                                                       <  1:3937119/150‑1 (MQ=255)
                                                                                                   cTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAc                                                    >  2:644952/1‑150 (MQ=255)
                                                                                                      caagcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAcccc                                                 >  1:3512533/1‑150 (MQ=255)
                                                                                                         gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCACGTACGACATCGCATTCACCCCGTc                                              >  1:4257139/1‑150 (MQ=255)
                                                                                                         gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                              >  1:4704232/1‑150 (MQ=255)
                                                                                                         gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                              >  1:5382942/1‑150 (MQ=255)
                                                                                                         gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                              <  2:2798389/150‑1 (MQ=255)
                                                                                                         gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCACGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                              >  2:3080650/1‑150 (MQ=255)
                                                                                                               tcgaaaccTGTCAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               <  2:4336936/106‑1 (MQ=255)
                                                                                                                gacCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                       >  1:3581710/1‑150 (MQ=255)
                                                                                                                gacCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                       >  2:1476702/1‑150 (MQ=255)
                                                                                                                gacCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                       <  1:1727877/150‑1 (MQ=255)
                                                                                                                  ccaccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATc                                     >  2:2365741/1‑150 (MQ=255)
                                                                                                                   caccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCg                                    >  1:2981865/1‑150 (MQ=255)
                                                                                                                   caccTGACAGGTTTCGACACCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCg                                    >  1:1202786/1‑150 (MQ=255)
                                                                                                                    accTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGc                                   >  2:1727440/1‑150 (MQ=255)
                                                                                                                        gACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcac                               >  2:4534065/1‑150 (MQ=255)
                                                                                                                        gACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcac                               >  2:4534288/1‑150 (MQ=255)
                                                                                                                             gTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCg                          >  2:4874234/1‑150 (MQ=255)
                                                                                                                             gTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCg                          >  1:3654392/1‑150 (MQ=255)
                                                                                                                             gTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCg                          >  1:2486147/1‑150 (MQ=255)
                                                                                                                              tttCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGa                         <  2:4123975/150‑1 (MQ=255)
                                                                                                                                 cGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACgg                      <  1:4987047/150‑1 (MQ=255)
                                                                                                                                  gACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGc                     >  2:5085282/1‑150 (MQ=255)
                                                                                                                                  gACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGc                     >  2:4943492/1‑150 (MQ=255)
                                                                                                                                   aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGcc                    >  2:3972941/1‑150 (MQ=255)
                                                                                                                                   aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGcc                    >  2:4458056/1‑150 (MQ=255)
                                                                                                                                     aTCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCg                  >  2:2780690/1‑150 (MQ=255)
                                                                                                                                     aTCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACACCGTCGAGTGAATCGCTCACAGGCGACGGCCCg                  >  2:240912/1‑150 (MQ=255)
                                                                                                                                      tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGc                 <  1:3376944/150‑1 (MQ=255)
                                                                                                                                      tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGc                 >  2:911180/1‑150 (MQ=255)
                                                                                                                                      tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGc                 >  1:3603293/1‑150 (MQ=255)
                                                                                                                                      tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGc                 >  1:4230415/1‑150 (MQ=255)
                                                                                                                                         aaTTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCg              >  2:79613/1‑150 (MQ=255)
                                                                                                                                          aTTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGa             >  1:734122/1‑150 (MQ=255)
                                                                                                                                           tttGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAt            <  1:3972941/150‑1 (MQ=255)
                                                                                                                                           cttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAt            >  1:5073244/2‑150 (MQ=255)
                                                                                                                                            ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGt                                          >  1:2640907/1‑119 (MQ=255)
                                                                                                                                            ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGt                                          <  2:2640907/119‑1 (MQ=255)
                                                                                                                                               gCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTaa        <  2:5382672/150‑1 (MQ=255)
                                                                                                                                                 cAGGGCCTGGCCAAAGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGc      >  2:2668975/1‑150 (MQ=255)
                                                                                                                                                 cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGc      >  2:2102011/1‑150 (MQ=255)
                                                                                                                                                   ggCCCTGGCCAAGGAACTGGAGTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCt    <  1:4834339/150‑1 (MQ=255)
                                                                                                                                                     cccTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGc  >  1:852168/1‑150 (MQ=255)
                                                                                                                                                     cccTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGc  <  1:2138071/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTACTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGC  >  NC_002947/5885424‑5885722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑