Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,921,615 A→C 7.4% K75Q (AAG→CAG) ‡ PP_5190 → type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,921,6150AC 7.4% 504.3 / 21.2 176K75Q (AAG→CAG) ‡PP_5190type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base A (83/79);  new base C (9/5);  total (92/84)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CGCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCT  >  NC_002947/5921467‑5921764
                                                                                                                                                    |                                                                                                                                                     
cGCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACaa                                                                                                                                                      <  1:1499005/150‑1 (MQ=255)
 gCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAg                                                                                                                                                     >  2:354297/1‑150 (MQ=255)
  ccAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAgg                                                                                                                                                    <  2:2983453/150‑1 (MQ=255)
     gCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCg                                                                                                                                                 <  2:4940180/150‑1 (MQ=255)
        aTACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACCTTGTCGGcc                                                                                                                                              >  2:3660665/1‑150 (MQ=255)
         tACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACCTTGTCGGCCa                                                                                                                                             <  1:3660665/150‑1 (MQ=255)
         tACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACCTTGTCGGCCa                                                                                                                                             <  2:5263388/150‑1 (MQ=255)
            ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGGCAAGGTCGGCCAAcc                                                                                                                                          >  1:2329357/1‑150 (MQ=255)
            ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAAcc                                                                                                                                          <  2:1890773/150‑1 (MQ=255)
            ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAAcc                                                                                                                                          >  1:1890773/1‑150 (MQ=255)
             cTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCAACAAGGTCGGCCAAccc                                                                                                                                         >  1:3974916/1‑150 (MQ=255)
                   gTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTAccgg                                                                                                                                   >  1:372730/1‑148 (MQ=255)
                    tACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTgc                                                                                                                                  <  1:1271239/150‑1 (MQ=255)
                       tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCa                                                                                                                               >  2:478967/1‑150 (MQ=255)
                       tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCGACCAGCGCa                                                                                                                               >  1:3795217/1‑150 (MQ=255)
                       tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCAACCTGCGCa                                                                                                                               >  1:3364132/1‑150 (MQ=255)
                       tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGGCGGCCCCCCCGACCGGCGCa                                                                                                                               >  1:972229/1‑150 (MQ=255)
                         gAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATc                                                                                                                             >  1:1628505/1‑150 (MQ=255)
                            cacGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGAc                                                                                                                          >  2:4636331/1‑150 (MQ=255)
                                ccgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCa                                                                                                                      >  2:4917295/1‑150 (MQ=255)
                                 cgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCAt                                                                                                                     <  1:4519421/150‑1 (MQ=255)
                                  gcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATg                                                                                                                    <  2:2286841/150‑1 (MQ=255)
                                         aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCg                                                                                                             >  1:506340/1‑150 (MQ=255)
                                         aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCg                                                                                                             >  1:5238367/1‑150 (MQ=255)
                                             cGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCt                                                                                                         <  2:3086398/150‑1 (MQ=255)
                                                    cACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAg                                                                                                  <  2:1625496/150‑1 (MQ=255)
                                                      cccGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGt                                                                                                >  2:4596759/1‑150 (MQ=255)
                                                      cccGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGt                                                                                                <  1:2527088/150‑1 (MQ=255)
                                                          cTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCAcc                                                                                            <  2:2765394/150‑1 (MQ=255)
                                                             gAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGc                                                                                         >  2:1446629/1‑150 (MQ=255)
                                                              aGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGcc                                                                                        <  1:4626209/150‑1 (MQ=255)
                                                              aGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGcc                                                                                        <  1:2069501/150‑1 (MQ=255)
                                                               gCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCt                                                                                       <  1:5229851/150‑1 (MQ=255)
                                                               gCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCt                                                                                       <  1:4987595/150‑1 (MQ=255)
                                                               gCACATCGCCGCCAGCAGCCTGGAAACCCGCCACCACCCCCCCCACCCCCAGGACCAGGAGCACCTTTGCCAGTGGCTGGCCGACAACCTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACACGACCAGCCCCACGTCACCAGCCt                                                                                       <  2:1403262/150‑1 (MQ=255)
                                                                  caTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAt                                                                                    <  1:213744/150‑1 (MQ=255)
                                                                   aTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAc                                                                                                           >  2:2444257/1‑126 (MQ=255)
                                                                    tCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtct                                                                                  >  2:823996/1‑150 (MQ=255)
                                                                    tCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtct                                                                                  >  2:1697990/1‑150 (MQ=255)
                                                                      gccgccTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTcc                                                                                >  2:4755847/1‑150 (MQ=255)
                                                                            tGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGcc                                                                          <  1:1009533/150‑1 (MQ=255)
                                                                              cAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCCACCCTACCTGCGCATCGACCCCATGCAACTCGACCAGCCCCAGGTCACCCGCCTGATCTCCCCAGCCtt                                                                        >  1:4767695/1‑150 (MQ=255)
                                                                                gCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCCTGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCg                                                                      >  2:922710/1‑150 (MQ=255)
                                                                                   tGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCCACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGccc                                                                   >  1:5348039/1‑150 (MQ=255)
                                                                                      aaaGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAgc                                                                <  1:4023670/150‑1 (MQ=255)
                                                                                        aGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAgcgc                                                              <  2:3656194/150‑1 (MQ=255)
                                                                                            gccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACg                                                          >  2:5201277/1‑150 (MQ=255)
                                                                                            gccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACg                                                          <  2:1208868/150‑1 (MQ=255)
                                                                                            gccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACg                                                          <  2:1926345/150‑1 (MQ=255)
                                                                                             ccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACgg                                                         >  2:3935098/1‑150 (MQ=255)
                                                                                               aGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCGGGCCGCCCAGGGCGGCCCCCCCGACCGGCGCCTCGCCCCCCGGCAACGCGCCCAGCCCCAGGTCACCCGCCGGCTCGCCCCAGCCGTCGCCCCGCGCCCCGGcc                                                       >  1:3687524/1‑149 (MQ=255)
                                                                                                gCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCAt                                                      <  1:5443454/150‑1 (MQ=255)
                                                                                                 cACCCCGGCCTGCCCCTGGACTTTGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  2:662660/150‑1 (MQ=255)
                                                                                                 cACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  2:4057870/150‑1 (MQ=255)
                                                                                                 cACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  1:5449496/150‑1 (MQ=255)
                                                                                                  aCCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCCCGGCATcc                                                    >  2:3685099/1‑150 (MQ=255)
                                                                                                  aCCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATcc                                                    <  1:5421721/150‑1 (MQ=255)
                                                                                                    cccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTc                                                  >  2:730366/1‑150 (MQ=255)
                                                                                                    cccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCAACGACCCCCAGCAACACCACCTGCCCCAGGTCACCACCCTGATCTCCCCAGCCATAGCCCAGCCCCACGGCAACCTc                                                  >  1:4753758/1‑150 (MQ=255)
                                                                                                    cccGGCCAGCCACTGCAAATGCATCACCTATGCCAGTGGCACGCCCACACGGTCGACCAACCCTGCCACCGCATAGACCACAAGCAACTAGAACTGCCCCAGGTCACAAGCCTGTTATCCCCAGCCTTCGCCCAGCGCCACGGCATACTc                                                  >  2:4794014/1‑150 (MQ=255)
                                                                                                       ggCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCAtc                                               <  2:4154561/150‑1 (MQ=255)
                                                                                                        gCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                       >  2:84074/1‑93 (MQ=255)
                                                                                                        gCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                       <  1:84074/93‑1 (MQ=255)
                                                                                                               ccTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGc                                       <  2:2650679/150‑1 (MQ=255)
                                                                                                                cTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGTCAAGGTGGGGCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTGACCAGCCTGATGTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGcc                                      >  1:4368513/1‑150 (MQ=255)
                                                                                                                 tGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCg                                     <  2:1628505/150‑1 (MQ=255)
                                                                                                                   gACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                   <  2:646127/150‑1 (MQ=255)
                                                                                                                   gACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCCCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                   <  2:493945/150‑1 (MQ=255)
                                                                                                                        ggAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTc                              <  2:4505590/150‑1 (MQ=255)
                                                                                                                        ggAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCAGCGCATCGACCCCAAGCAACACGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCACCGCCACCGCAACCACATCGTCGCGGCCGATGCCTc                              >  1:1167628/1‑150 (MQ=255)
                                                                                                                          aGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCa                            <  2:2129987/150‑1 (MQ=255)
                                                                                                                           gCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAg                           >  2:3463901/1‑150 (MQ=255)
                                                                                                                               cTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCGGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       <  2:5238367/150‑1 (MQ=255)
                                                                                                                               cTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       <  1:595452/150‑1 (MQ=255)
                                                                                                                                tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      >  2:817279/1‑150 (MQ=255)
                                                                                                                                tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      <  2:2885662/150‑1 (MQ=255)
                                                                                                                                tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      <  1:144001/150‑1 (MQ=255)
                                                                                                                                tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      >  2:5160645/1‑150 (MQ=255)
                                                                                                                                  tGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCAcc                    <  2:4053902/150‑1 (MQ=255)
                                                                                                                                       gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGcc                                      >  2:4321350/1‑127 (MQ=255)
                                                                                                                                       gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGcc                                      <  1:4321350/127‑1 (MQ=255)
                                                                                                                                       gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGc               <  1:2700944/150‑1 (MQ=255)
                                                                                                                                       gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGc               <  1:1422856/150‑1 (MQ=255)
                                                                                                                                         ggctggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCa             <  2:5130959/150‑1 (MQ=255)
                                                                                                                                          gttggcCGACCTTGTCGGCCAGCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGc                          >  1:91579/3‑136 (MQ=255)
                                                                                                                                          gttggcCGACCTTGTCGGCCAGCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGc                          <  2:91328/134‑1 (MQ=255)
                                                                                                                                          gctggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCACCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAg            >  2:5340607/1‑150 (MQ=255)
                                                                                                                                           ttggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc           >  2:4035805/2‑150 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCa                            <  1:1107584/132‑1 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCa                            >  2:1107584/1‑132 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg          >  1:3417063/1‑150 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg          <  1:4834474/150‑1 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg          >  1:696521/1‑150 (MQ=255)
                                                                                                                                            tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg          >  1:4061702/1‑150 (MQ=255)
                                                                                                                                             ggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGTCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       <  1:2646483/136‑1 (MQ=255)
                                                                                                                                             ggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGTCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       >  2:2646483/1‑136 (MQ=255)
                                                                                                                                                cGACAAGGTCGGCCAACCCTACCTGCGCATCTACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGccc                                                                   >  1:3004087/1‑89 (MQ=255)
                                                                                                                                                cGACAAGGTCGGCCAACCCTACCTGCGCATCCACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGccc                                                                   <  2:3004087/89‑1 (MQ=255)
                                                                                                                                                  aCAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCCGc    >  1:4785948/1‑150 (MQ=255)
                                                                                                                                                  aCAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGc    >  1:4786034/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCT  >  NC_002947/5921467‑5921764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑