Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,921,617 G→T 5.8% K75N (AAG→AAT) ‡ PP_5190 → type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,921,6170GT 5.8% 526.6 / 17.8 174K75N (AAG→AAT) ‡PP_5190type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base G (86/77);  new base T (5/5);  total (91/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.38e-01

Only 100 of 177 total aligned reads displayed.

GCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCT  >  NC_002947/5921468‑5921764
                                                                                                                                                     |                                                                                                                                                   
gCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAg                                                                                                                                                     >  2:354297/1‑150 (MQ=255)
 ccAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAgg                                                                                                                                                    <  2:2983453/150‑1 (MQ=255)
  cAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGt                                                                                                                                                   >  2:5449496/1‑150 (MQ=255)
   aGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTc                                                                                                                                                  >  1:5437913/1‑150 (MQ=255)
    gCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCg                                                                                                                                                 <  1:3763283/150‑1 (MQ=255)
    gCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCg                                                                                                                                                 <  2:4940180/150‑1 (MQ=255)
      gATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGc                                                                                                                                               <  1:1612295/150‑1 (MQ=255)
      gATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGc                                                                                                                                               <  2:2040247/150‑1 (MQ=255)
      gATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGc                                                                                                                                               <  1:683276/150‑1 (MQ=255)
        tACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACCTTGTCGGCCa                                                                                                                                             <  2:5263388/150‑1 (MQ=255)
        tACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACCTTGTCGGCCa                                                                                                                                             <  1:3660665/150‑1 (MQ=255)
           ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAAcc                                                                                                                                          <  2:1890773/150‑1 (MQ=255)
            cTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCAACAAGGTCGGCCAAccc                                                                                                                                         >  1:3974916/1‑150 (MQ=255)
              gCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAAcccaa                                                                                                                                       >  1:5052115/1‑148 (MQ=255)
                aaGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTAcc                                                                                                                                     <  1:5330506/150‑1 (MQ=255)
                   tACTCGAACACTCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTgc                                                                                                                                  <  1:194666/150‑1 (MQ=255)
                   tACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTgc                                                                                                                                  <  1:1271239/150‑1 (MQ=255)
                      tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCACCCCTACCTgcgcc                                                                                                                               >  2:4631782/1‑149 (MQ=255)
                      tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCa                                                                                                                               >  2:478967/1‑150 (MQ=255)
                      tCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCGTTGCCAGTGGCTGGCCGACAAGGTCGGCCCACCCTACCTGCGCa                                                                                                                               >  1:2967610/1‑150 (MQ=255)
                        gAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATc                                                                                                                             >  2:4834474/1‑150 (MQ=255)
                         aaCACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCg                                                                                                                            >  1:5400936/1‑150 (MQ=255)
                           cacGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGAc                                                                                                                          >  2:4636331/1‑150 (MQ=255)
                            acGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATTGAcc                                                                                                                         <  1:2560955/150‑1 (MQ=255)
                               ccgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCa                                                                                                                      >  2:4917295/1‑150 (MQ=255)
                                cgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCAt                                                                                                                     >  1:4052442/1‑150 (MQ=255)
                                cgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCAt                                                                                                                     <  1:4519421/150‑1 (MQ=255)
                                 gcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATg                                                                                                                    >  1:4943185/1‑150 (MQ=255)
                                 gcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATg                                                                                                                    <  2:2286841/150‑1 (MQ=255)
                                     cccACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAAc                                                                                                                <  1:2221783/150‑1 (MQ=255)
                                        aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCg                                                                                                             >  1:506340/1‑150 (MQ=255)
                                          cccGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAc                                                                                                           <  1:3383104/150‑1 (MQ=255)
                                                   cACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAg                                                                                                  <  2:1625881/150‑1 (MQ=255)
                                                    aCCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAgg                                                                                                 >  2:2700944/1‑150 (MQ=255)
                                                        gCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCAc                                                                                             <  2:2152424/150‑1 (MQ=255)
                                                         cTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCAcc                                                                                            <  2:2765394/150‑1 (MQ=255)
                                                            gAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGc                                                                                         >  2:1446629/1‑150 (MQ=255)
                                                            gAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGc                                                                                         <  2:3667654/150‑1 (MQ=255)
                                                             aGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGcc                                                                                        <  1:2069501/150‑1 (MQ=255)
                                                              gCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCt                                                                                       <  1:5229851/150‑1 (MQ=255)
                                                              gCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCt                                                                                       >  1:332084/1‑150 (MQ=255)
                                                              gCACATCGCCGCCAGCAGCCTGGAAACCCGCCACCACCCCCCCCACCCCCAGGACCAGGAGCACCTTTGCCAGTGGCTGGCCGACAACCTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACACGACCAGCCCCACGTCACCAGCCt                                                                                       <  2:1403262/150‑1 (MQ=255)
                                                               cacaTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACACGACCTGCCCCAGGTCACCAGCCTg                                                                                      >  1:1685607/1‑150 (MQ=255)
                                                                  aTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAc                                                                                                           >  2:2444257/1‑126 (MQ=255)
                                                                  aTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAc                                                                                                           <  1:2444257/126‑1 (MQ=255)
                                                                  aTCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACGTGGAGCACCTTTGCCAGTGGCTGGCCGCCAAGGTCGGCCAACCCTACCTGCGCCGCGACCCCAGGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtc                                                                                   >  2:1198813/1‑150 (MQ=255)
                                                                   tCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtct                                                                                  >  2:823996/1‑150 (MQ=255)
                                                                   tCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtct                                                                                  >  2:1697990/1‑150 (MQ=255)
                                                                     gccgccTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTcc                                                                                >  2:4755847/1‑150 (MQ=255)
                                                                           tGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGcc                                                                          <  1:1009533/150‑1 (MQ=255)
                                                                               gCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCCTGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCg                                                                      >  2:922710/1‑150 (MQ=255)
                                                                                ccTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGc                                                                     <  1:354297/150‑1 (MQ=255)
                                                                                   ggAAAGCCGCCAGCACCCCGACCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGGCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCa                                                                  >  1:3889460/1‑150 (MQ=255)
                                                                                    gAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCAACCAGCGCAACGACCCCAAGCAACACGACCTGCCCCAGGTCACCAGCCAGATCTACCCAGCCATCGCCCac                                                                 >  1:4651219/1‑149 (MQ=255)
                                                                                       aGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAgcgc                                                              <  2:3656194/150‑1 (MQ=255)
                                                                                         ccgccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCCTCGCCCCCATGCAACTCGACCGGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGccc                                                            >  1:4298215/1‑149 (MQ=255)
                                                                                          cgccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCAc                                                           <  2:332084/150‑1 (MQ=255)
                                                                                           gccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACg                                                          >  2:5201277/1‑150 (MQ=255)
                                                                                           gccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACg                                                          <  2:1208868/150‑1 (MQ=255)
                                                                                            ccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACgg                                                         >  2:16476/1‑150 (MQ=255)
                                                                                            ccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACgg                                                         >  2:3935098/1‑150 (MQ=255)
                                                                                               gCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCAt                                                      <  1:5443454/150‑1 (MQ=255)
                                                                                                cACCCCGGCCTGCCCCTGGACTTTGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  2:662660/150‑1 (MQ=255)
                                                                                                cACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  2:4057870/150‑1 (MQ=255)
                                                                                                cACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCAGGCAACTCGACCTTCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                     <  2:4057848/150‑1 (MQ=255)
                                                                                                 aCCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCCCGGCATcc                                                    >  2:3685099/1‑150 (MQ=255)
                                                                                                   cccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTc                                                  >  2:730366/1‑150 (MQ=255)
                                                                                                   cccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTAACTGCGCATCGACCCCATGCAACTCGACCAGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTc                                                  >  1:3702252/1‑150 (MQ=255)
                                                                                                    ccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCa                                                 <  2:3080289/150‑1 (MQ=255)
                                                                                                    ccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAAACCAACCGGCGCAACGACCCCAAGCAACACGACCAGCCCCAGGTCACCAGCCAGATCTCCCCAGCCATAGCCCCGCGCCACCGccaccaca                                                 >  1:593840/1‑143 (MQ=255)
                                                                                                      ggCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCAtc                                               <  2:4154561/150‑1 (MQ=255)
                                                                                                       gCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                       >  2:84074/1‑93 (MQ=255)
                                                                                                       gCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                       <  1:84074/93‑1 (MQ=255)
                                                                                                                  gACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                   <  2:646127/150‑1 (MQ=255)
                                                                                                                  gACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCCCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                   <  2:493945/150‑1 (MQ=255)
                                                                                                                      tGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCt                               <  1:982260/150‑1 (MQ=255)
                                                                                                                       ggAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTc                              <  2:4505590/150‑1 (MQ=255)
                                                                                                                       ggAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCAGCGCATCGACCCCAAGCAACACGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCACCGCCACCGCAACCACATCGTCGCGGCCGATGCCTc                              >  1:1167628/1‑150 (MQ=255)
                                                                                                                         aGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCa                            <  1:433967/150‑1 (MQ=255)
                                                                                                                          gCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAg                           >  2:3463901/1‑150 (MQ=255)
                                                                                                                              cTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCGGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       <  2:5238367/150‑1 (MQ=255)
                                                                                                                               tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      <  1:144001/150‑1 (MQ=255)
                                                                                                                               tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      <  2:2885662/150‑1 (MQ=255)
                                                                                                                               tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      >  2:817279/1‑150 (MQ=255)
                                                                                                                               tttGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCa                      >  2:5160645/1‑150 (MQ=255)
                                                                                                                                   ccAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGt                  <  1:478967/150‑1 (MQ=255)
                                                                                                                                      gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGcc                                      >  2:4321350/1‑127 (MQ=255)
                                                                                                                                      gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGc               <  1:2700944/150‑1 (MQ=255)
                                                                                                                                      gTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGc               <  1:1422856/150‑1 (MQ=255)
                                                                                                                                        ggctggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCa             <  2:5130959/150‑1 (MQ=255)
                                                                                                                                        ggctggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCa             <  1:4768732/150‑1 (MQ=255)
                                                                                                                                         gttggcCGACCTTGTCGGCCAGCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGc                          >  1:91579/3‑136 (MQ=255)
                                                                                                                                         gttggcCGACCTTGTCGGCCAGCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGc                          <  2:91328/134‑1 (MQ=255)
                                                                                                                                          ttggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc           >  2:4035805/2‑150 (MQ=255)
                                                                                                                                           tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCa                            <  1:1107584/132‑1 (MQ=255)
                                                                                                                                           tggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg          >  1:696562/1‑150 (MQ=255)
                                                                                                                                            ggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGTCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTc                       >  2:2646483/1‑136 (MQ=255)
                                                                                                                                                 aCAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCCGc    >  1:4785948/1‑150 (MQ=255)
                                                                                                                                                 aCAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGc    <  1:4755847/150‑1 (MQ=255)
                                                                                                                                                 aCAAGGGCGGCCAACCCTACCTGCGCATCGACCCCCTGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGc    <  1:4416170/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCAGCGATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCT  >  NC_002947/5921468‑5921764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑