BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,971,716	T→C	9.2%	K519R (AAG→AGG)	PP_5236 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,971,716	0	T	C	9.2%	475.1 / 26.9	163	K519R (AAG→AGG)	PP_5236	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (71/76); new base C (6/9); total (77/86)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 167 total aligned reads displayed.

GCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGCGCAG  >  NC_002947/5971568‑5971865

gCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACtt                                                                                                                                                      <  1:3896706/150‑1 (MQ=255)

gCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACtt                                                                                                                                                      >  2:1342139/1‑150 (MQ=255)

  gTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTgg                                                                                                                                                    <  1:2767608/150‑1 (MQ=255)

  gTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTgg                                                                                                                                                    >  1:4103881/1‑150 (MQ=255)

   tAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgc                                                                                                                                                   <  2:3699488/150‑1 (MQ=255)

    aaCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcc                                                                                                                                                  >  1:4526770/1‑150 (MQ=255)

    aaCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcc                                                                                                                                                  <  1:2500132/150‑1 (MQ=255)

    aaCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcc                                                                                                                                                  <  1:2500129/150‑1 (MQ=255)

     aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                                 >  2:5272336/1‑150 (MQ=255)

     aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcct                                                                                                                                                 <  1:2198535/150‑1 (MQ=255)

     aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcct                                                                                                                                                 <  1:1270130/150‑1 (MQ=255)

      cAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctg                                                                                                                                                >  2:4067264/1‑150 (MQ=255)

      cAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctg                                                                                                                                                >  1:2969579/1‑150 (MQ=255)

      cAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctg                                                                                                                                                >  2:2648455/1‑150 (MQ=255)

       aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                               <  1:5256918/150‑1 (MQ=255)

       aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                               >  1:3338656/1‑150 (MQ=255)

       aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                               <  2:245913/150‑1 (MQ=255)

       aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                               <  2:246012/150‑1 (MQ=255)

       aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcctgc                                                                                                                                               <  2:5289309/150‑1 (MQ=255)

        aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                              <  1:769907/150‑1 (MQ=255)

         atgtcccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcct                                                                                                                                             <  1:158660/146‑1 (MQ=255)

              ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGGAGGCCAAGTACAACCTGgcctgc                                                                                                                                               <  2:3839628/143‑1 (MQ=255)

              ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                                        >  1:1229074/1‑150 (MQ=255)

              ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                                        >  1:1766336/1‑150 (MQ=255)

              ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGAAGGCCAAGTACAACCTGgcctgc                                                                                                                                               >  1:3839628/1‑143 (MQ=255)

                  gTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACACGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGc                                                                                                                                    <  2:2391405/150‑1 (MQ=255)

                   tGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGcc                                                                                                                                   <  2:4862452/150‑1 (MQ=255)

                      aaCCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTcc                                                                                                                                >  2:5351403/1‑150 (MQ=255)

                         cgcgCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCAcc                                                                                                                             <  2:2997173/150‑1 (MQ=255)

                         cgcgCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCAcc                                                                                                                             >  2:5331418/1‑150 (MQ=255)

                             cacaCGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCAc                                                                                                                         >  1:1433466/1‑150 (MQ=255)

                              acacGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACg                                                                                                                        <  2:1433466/150‑1 (MQ=255)

                              acacGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACg                                                                                                                        >  2:2116876/1‑150 (MQ=255)

                                acGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCt                                                                                                                      >  1:1024081/1‑150 (MQ=255)

                                  gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                                    >  1:3932811/1‑150 (MQ=255)

                                  gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                                    >  1:5129586/1‑150 (MQ=255)

                                   cATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCa                                                                                                                   <  2:3208490/150‑1 (MQ=255)

                                     ttGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGc                                                                                                                 <  2:5093198/150‑1 (MQ=255)

                                       ggTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                               <  1:5399063/150‑1 (MQ=255)

                                        gTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAg                                                                                                              >  2:782198/1‑150 (MQ=255)

                                        gTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGAGCAGGCAGGCCAAGTACAACCTGgcctgc                                                                                                                                               >  1:5115156/1‑117 (MQ=38)

                                                     ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                                 >  1:2844861/1‑150 (MQ=255)

                                                      cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCTTGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                                <  1:3013681/150‑1 (MQ=255)

                                                      cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCTGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                                <  1:4097169/150‑1 (MQ=255)

                                                      cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                                <  1:889584/150‑1 (MQ=255)

                                                       aGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCg                                                                                               <  2:4343842/150‑1 (MQ=255)

                                                            gTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATc                                                                                          <  1:4887604/150‑1 (MQ=255)

                                                                aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                                      <  2:2724132/150‑1 (MQ=255)

                                                                  aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcct                                                                                                                                             <  2:2736853/93‑1 (MQ=255)

                                                                  aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                                    >  1:5352852/1‑150 (MQ=255)

                                                                    gCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAAt                                                                                  >  1:5368213/1‑150 (MQ=255)

                                                                    gCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAAt                                                                                  >  1:4913804/1‑150 (MQ=255)

                                                                         cgcCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGcc                                                                             >  2:1819839/1‑150 (MQ=255)

                                                                            cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                          <  1:4964167/150‑1 (MQ=255)

                                                                            cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                          <  2:2302813/150‑1 (MQ=255)

                                                                                  gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                                    <  1:1103434/150‑1 (MQ=255)

                                                                                     aGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGcc                                                                 >  2:3771808/1‑150 (MQ=255)

                                                                                             aCTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgcc                                                         >  2:3108626/1‑150 (MQ=255)

                                                                                              cTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCa                                                        <  1:5413371/150‑1 (MQ=255)

                                                                                               tGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCAt                                                       >  2:2214085/1‑150 (MQ=255)

                                                                                               tGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCAt                                                       >  2:2214270/1‑150 (MQ=255)

                                                                                               tGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCAt                                                       <  1:81657/150‑1 (MQ=255)

                                                                                                 cgcTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATgg                                                     <  1:4269338/150‑1 (MQ=255)

                                                                                                   cTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTg                                                   >  2:4106946/1‑150 (MQ=255)

                                                                                                      gTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCg                                                                            >  2:1078564/1‑122 (MQ=255)

                                                                                                      gTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAg                                                >  1:3558117/1‑150 (MQ=255)

                                                                                                      gTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAg                                                >  1:4640976/1‑150 (MQ=255)

                                                                                                      gTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAg                                                >  1:4694747/1‑150 (MQ=255)

                                                                                                       ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                               >  1:3694666/1‑150 (MQ=255)

                                                                                                          aGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCa                                            <  1:4727427/150‑1 (MQ=255)

                                                                                                             tCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATAc                                         >  1:4845842/1‑150 (MQ=255)

                                                                                                              ccTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACa                                        >  1:1614752/1‑150 (MQ=255)

                                                                                                               cTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACaa                                       <  2:1080732/150‑1 (MQ=255)

                                                                                                                tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATcc                                                                                         <  1:1007244/99‑1 (MQ=255)

                                                                                                                tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTg                                                   >  1:2057099/1‑137 (MQ=255)

                                                                                                                tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                                      <  2:4644621/150‑1 (MQ=255)

                                                                                                                       cGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAggg                               <  2:3699397/150‑1 (MQ=255)

                                                                                                                        gTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgc                              <  2:5352852/150‑1 (MQ=255)

                                                                                                                        gTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgc                              >  1:4770459/1‑150 (MQ=255)

                                                                                                                          ccATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCCGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcag                            <  2:1333805/150‑1 (MQ=255)

                                                                                                                            aTGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCCTACAAGGCCAGGgcagca                          >  2:4010535/1‑150 (MQ=255)

                                                                                                                              gcgcAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCAcc                        <  2:1229074/150‑1 (MQ=255)

                                                                                                                               cgcAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCAccc                       >  2:4813494/1‑150 (MQ=255)

                                                                                                                                 caggcaggCCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCgg                     >  2:3348290/1‑150 (MQ=255)

                                                                                                                                 caggcaggCCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCgg                     >  1:2982395/1‑150 (MQ=255)

                                                                                                                                   ggcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCa                   <  2:1664965/150‑1 (MQ=255)

                                                                                                                                    gcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGGACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg                  <  1:3683535/150‑1 (MQ=255)

                                                                                                                                    gcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCa                                            <  1:856930/124‑1 (MQ=255)

                                                                                                                                    gcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCa                                            >  2:856930/1‑124 (MQ=255)

                                                                                                                                       ggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGtt               <  1:4393614/150‑1 (MQ=255)

                                                                                                                                        gcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTc              <  2:4103881/150‑1 (MQ=255)

                                                                                                                                        gcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTc              <  1:2260771/150‑1 (MQ=255)

                                                                                                                                         cctggTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCa             <  1:2116876/147‑1 (MQ=255)

                                                                                                                                          cAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAg            <  2:4526770/150‑1 (MQ=255)

                                                                                                                                              ttGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCg        <  2:4913804/150‑1 (MQ=255)

                                                                                                                                               tGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCgg       >  2:2118178/1‑150 (MQ=255)

                                                                                                                                                gTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGgc      <  2:1994981/150‑1 (MQ=255)

                                                                                                                                                   cTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGCGCa   <  1:1225520/150‑1 (MQ=255)

                                                                                                                                                   cTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGCGCa   <  1:2671502/150‑1 (MQ=255)

                                                                                                                                                    ttGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGCGCAg  >  1:1744774/1‑150 (MQ=255)

GCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGCGCAG  >  NC_002947/5971568‑5971865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`