BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,852	G→A	5.4%	Y82Y (TAC→TAT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,852	0	G	A	5.4%	737.4 / 18.5	222	Y82Y (TAC→TAT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (107/103); new base A (6/6); total (113/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 223 total aligned reads displayed.

AAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGC  >  NC_002947/5986703‑5986999

 aaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGt                                                                                                                                                    >  1:1066887/1‑150 (MQ=255)

  aCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTa                                                                                                                                                   <  1:3810572/150‑1 (MQ=255)

   ccTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTAc                                                                                                                                                  >  1:134835/1‑150 (MQ=255)

     ttCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACgg                                                                                                                                                >  2:2257162/1‑150 (MQ=255)

      tCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGTTGATAAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGgc                                                                                                                                               <  2:2335580/150‑1 (MQ=255)

             gcgcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCg                                                                                                                                        <  2:1872624/150‑1 (MQ=255)

              cgcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                                                       <  2:1317477/150‑1 (MQ=255)

              cgcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                                                       <  2:3560286/150‑1 (MQ=255)

               gcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCggg                                                                                                                                      <  1:3974005/150‑1 (MQ=255)

                ccGACAGGGTCGGGAAGGCCACGCACACGTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGa                                                                                                                                     <  1:242277/150‑1 (MQ=255)

                 cGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGaa                                                                                                                                    <  1:873628/150‑1 (MQ=255)

                 cGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGaa                                                                                                                                    <  1:2215330/150‑1 (MQ=255)

                       ggTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACAtctc                                                                                                                              >  2:701785/1‑150 (MQ=255)

                         tCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCtt                                                                                                                            >  1:4751573/1‑150 (MQ=255)

                         tCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCtt                                                                                                                            <  2:1385940/150‑1 (MQ=255)

                            ggAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                                                         >  2:2967296/1‑150 (MQ=255)

                                  ccACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                                                                   >  2:334945/1‑150 (MQ=255)

                                  ccACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                                                                   <  2:2877210/150‑1 (MQ=255)

                                  ccACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                                                                   <  2:816237/150‑1 (MQ=255)

                                    aCGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGgcgc                                                                                                                                             <  1:1439267/122‑1 (MQ=255)

                                      gCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCAc                                                                                                               <  1:265148/150‑1 (MQ=255)

                                       cacacaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCAcc                                                                                                              <  2:556841/150‑1 (MQ=255)

                                        acacaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCa                                                                                                             >  1:2463283/1‑150 (MQ=255)

                                        acacaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCGTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCa                                                                                                             >  1:3924575/1‑150 (MQ=255)

                                          acaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                                                          >  1:1861026/1‑135 (MQ=255)

                                                gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCa                                                                                                     >  1:1904466/1‑150 (MQ=255)

                                                   accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                                                       >  2:171194/1‑113 (MQ=255)

                                                   accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                                                       <  1:171194/113‑1 (MQ=255)

                                                   accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTc                                                                                                  >  1:1494926/1‑150 (MQ=255)

                                                    ccacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCATCAGCGGGCCATTCg                                                                                                 >  1:4436592/1‑150 (MQ=255)

                                                           ggCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTg                                                                                          >  1:5323263/1‑150 (MQ=255)

                                                            gCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                         >  2:4685383/1‑150 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       <  2:1033172/150‑1 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       <  2:81145/150‑1 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       <  2:5098566/150‑1 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       >  2:2422565/1‑150 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       >  1:730801/1‑150 (MQ=255)

                                                              gatgatCAGTTGCTTGCGGCCCGTGGCCTTGAGTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                       <  2:2997968/150‑1 (MQ=255)

                                                                tgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGa                                                                                     >  1:1664602/1‑150 (MQ=255)

                                                                    cAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCggg                                                                                               >  1:1238435/1‑136 (MQ=255)

                                                                       ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                              >  2:2400553/1‑150 (MQ=255)

                                                                        tgcgtgcgGCCCGGGGCCTTGAGCGCCTGGACGCACGCTTCGCTGTCCCAGGCGGGGCTCTGGCCGGGACGGGCACGGTACGGCGCATCCGGGAACATCTCGGTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                             <  2:1447138/150‑1 (MQ=18)

                                                                          cttcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggt                                                                           >  1:228457/7‑150 (MQ=0)

                                                                           ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                          >  1:3300048/1‑150 (MQ=255)

                                                                            tcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                         <  1:2368300/146‑3 (MQ=2)

                                                                            tcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                         <  2:1677880/146‑3 (MQ=2)

                                                                             cttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAg                                                                        >  1:2689952/4‑150 (MQ=2)

                                                                               ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                      >  1:3186651/1‑150 (MQ=255)

                                                                               ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                      >  2:3260243/1‑150 (MQ=255)

                                                                                gCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                     <  2:2249714/150‑1 (MQ=255)

                                                                                  cggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacag                                                                   >  1:233465/3‑145 (MQ=2)

                                                                                  cggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacag                                                                   <  2:1094744/148‑6 (MQ=2)

                                                                                  ccGTGGCCTTGATTGCCTTGACGAAGTGTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGGTCGAAGCTGGTGGTGAGGATgg                                                                   >  2:519439/1‑150 (MQ=255)

                                                                                   ggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacagt                                                                  <  1:988327/149‑7 (MQ=2)

                                                                                   cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                  <  1:1568358/150‑1 (MQ=255)

                                                                                   cGTGGCCTTGAGTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                  <  2:501809/150‑1 (MQ=255)

                                                                                    gTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                                                                 <  2:2707846/150‑1 (MQ=255)

                                                                                         cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTATGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                            >  2:3358551/1‑150 (MQ=255)

                                                                                         cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                            >  2:1195928/1‑150 (MQ=255)

                                                                                           tGATTGCCTTGACGAAGTCTACGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                                          <  2:1530726/150‑1 (MQ=255)

                                                                                            gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                                                         >  1:3508569/1‑86 (MQ=255)

                                                                                            gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                         >  1:1152366/1‑150 (MQ=255)

                                                                                            gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                         >  1:3366681/1‑150 (MQ=255)

                                                                                            gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                         >  1:4461564/1‑150 (MQ=255)

                                                                                             aTTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                                        <  2:5323263/150‑1 (MQ=255)

                                                                                                  cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATCCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                   >  2:4787440/1‑150 (MQ=255)

                                                                                                     gACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                                <  1:5070451/150‑1 (MQ=255)

                                                                                                      aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                               <  2:4428936/150‑1 (MQ=255)

                                                                                                        gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                             >  2:916448/1‑150 (MQ=255)

                                                                                                        gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                             >  1:3050567/1‑150 (MQ=255)

                                                                                                        gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                             <  1:690387/150‑1 (MQ=255)

                                                                                                        gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                             >  1:484980/1‑150 (MQ=255)

                                                                                                              ttCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                                       >  2:4300201/1‑150 (MQ=255)

                                                                                                                cGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGc                                     <  2:3881301/150‑1 (MQ=255)

                                                                                                                     tCCCAGGCGTTGATCTGGCCGGGACGGGCAATGGACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                                <  2:2521583/150‑1 (MQ=255)

                                                                                                                       ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                              <  2:800770/150‑1 (MQ=255)

                                                                                                                       ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                              >  1:2166912/1‑150 (MQ=255)

                                                                                                                       ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                              >  2:3202674/1‑150 (MQ=255)

                                                                                                                       ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCACTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                              >  2:4091009/1‑150 (MQ=255)

                                                                                                                        cAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                             >  1:364954/1‑150 (MQ=255)

                                                                                                                         aGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                                                          >  1:2787179/1‑56 (MQ=255)

                                                                                                                         aGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCa                            >  1:4647812/1‑150 (MQ=255)

                                                                                                                              ttGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                  <  2:2844605/107‑1 (MQ=255)

                                                                                                                              ttGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                  >  1:2844605/1‑107 (MQ=255)

                                                                                                                                   cTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                    <  1:491493/100‑1 (MQ=255)

                                                                                                                                    tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                 >  2:3431073/1‑150 (MQ=255)

                                                                                                                                    tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                 >  2:5449777/1‑150 (MQ=255)

                                                                                                                                      gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt               <  2:1132674/150‑1 (MQ=255)

                                                                                                                                      gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt               <  2:1234531/150‑1 (MQ=255)

                                                                                                                                      gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt               >  2:730341/1‑150 (MQ=255)

                                                                                                                                      gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt               <  2:2672134/150‑1 (MQ=255)

                                                                                                                                      gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt               <  1:1017128/150‑1 (MQ=255)

                                                                                                                                        cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa             <  2:2079222/150‑1 (MQ=255)

                                                                                                                                        cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa             >  1:111790/1‑150 (MQ=255)

                                                                                                                                         gggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGaa            <  2:2565656/150‑1 (MQ=255)

                                                                                                                                          ggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAAt           <  1:2257162/150‑1 (MQ=255)

                                                                                                                                                gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg     <  2:3874589/150‑1 (MQ=255)

                                                                                                                                                gCAATGTACGGCGCATCCGGGAACATCTCGTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg     <  1:1981191/150‑1 (MQ=255)

                                                                                                                                                 cAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTgg    <  2:3924246/150‑1 (MQ=255)

                                                                                                                                                   aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc  <  1:5445730/150‑1 (MQ=255)

AAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGC  >  NC_002947/5986703‑5986999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`