BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,893	G→A	6.0%	L69L (CTG→TTG)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,893	0	G	A	6.0%	660.5 / 19.1	200	L69L (CTG→TTG)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (95/93); new base A (6/6); total (101/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 201 total aligned reads displayed.

ATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGA  >  NC_002947/5986747‑5987042

 tCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCggg                                                                                                                                                   >  1:569471/1‑150 (MQ=255)

       accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTc                                                                                                                                             >  1:1494926/1‑150 (MQ=255)

        ccacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCg                                                                                                                                            >  1:2464487/1‑150 (MQ=255)

        ccacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCg                                                                                                                                            >  1:4436442/1‑150 (MQ=255)

           cacCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                                         <  1:2337252/150‑1 (MQ=255)

                gCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                                                                                    <  2:4699968/150‑1 (MQ=255)

                  gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                                  <  2:81145/150‑1 (MQ=255)

                  gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                                  >  1:730852/1‑150 (MQ=255)

                  gatgatCAGTTGCTTGCGGCCCGTGGCCTTGAGTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                                  <  2:2997968/150‑1 (MQ=255)

                     gatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                               <  2:2203079/150‑1 (MQ=255)

                        cAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCggg                                                                                                                                          >  1:1238435/1‑136 (MQ=255)

                         aGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCTTTCGGGCCTTGCTCGAAGCtg                                                                                                                           <  2:266410/150‑1 (MQ=255)

                           ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                                         >  2:2400553/1‑150 (MQ=255)

                            tgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                        <  1:3099415/150‑1 (MQ=255)

                            tgcttgcGGCCCGTGGCCGTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                        <  2:3924575/150‑1 (MQ=255)

                            tgcgtgcgGCCCGGGGCCTTGAGCGCCTGGACGCACGCTTCGCTGTCCCAGGCGGGGCTCTGGCCGGGACGGGCACGGTACGGCGCATCCGGGAACATCTCGGTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                        <  2:1447138/150‑1 (MQ=18)

                              cttcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggt                                                                                                                      >  1:228457/7‑150 (MQ=0)

                               ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  1:3300048/1‑150 (MQ=255)

                                tgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                    >  1:5045230/1‑150 (MQ=255)

                                tcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                                                                    <  2:1677880/146‑3 (MQ=2)

                                 cttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAg                                                                                                                   >  1:2689952/4‑150 (MQ=2)

                                 cttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAg                                                                                                                   >  1:543958/4‑150 (MQ=2)

                                   ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 >  1:3186651/1‑150 (MQ=255)

                                   ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 >  2:3308644/1‑150 (MQ=255)

                                    gCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                                <  2:2249714/150‑1 (MQ=255)

                                      cggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacag                                                                                                              <  2:1094744/148‑6 (MQ=2)

                                      ccGTGGCCTTGATTGCCTTGACGAAGTGTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGGTCGAAGCTGGTGGTGAGGATgg                                                                                                              >  2:519439/1‑150 (MQ=255)

                                       cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                             <  1:1568358/150‑1 (MQ=255)

                                       cGTGGCCTTGAGTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                             <  2:501809/150‑1 (MQ=255)

                                             cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTATGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                                                                       >  2:3358551/1‑150 (MQ=255)

                                             cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                                                                       >  2:1195928/1‑150 (MQ=255)

                                                gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                                                                    >  1:3366681/1‑150 (MQ=255)

                                                gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                                                                    >  1:4461564/1‑150 (MQ=255)

                                                 aTTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                                                                                   <  2:5323263/150‑1 (MQ=255)

                                                  ttGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaa                                                                                                  >  1:4654211/1‑150 (MQ=255)

                                                   tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                 <  2:2947662/150‑1 (MQ=255)

                                                    gCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgcttgaaa                                                                                                <  2:1542668/150‑8 (MQ=2)

                                                      cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                              >  1:3564477/1‑150 (MQ=255)

                                                      cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATCCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                              >  2:4787440/1‑150 (MQ=255)

                                                          aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                          >  1:3657046/1‑150 (MQ=255)

                                                          aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                          <  2:4428936/150‑1 (MQ=255)

                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                        >  1:1622012/1‑150 (MQ=255)

                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                        >  1:2306542/1‑150 (MQ=255)

                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                        <  1:690387/150‑1 (MQ=255)

                                                             aaGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGcc                                                                                       <  1:1824828/150‑1 (MQ=255)

                                                               gTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                                                                                     >  2:319881/1‑150 (MQ=255)

                                                                  ttCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                                                                                  >  2:912689/1‑150 (MQ=255)

                                                                    cGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGc                                                                                <  2:3881301/150‑1 (MQ=255)

                                                                    cGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATCCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGc                                                                                <  1:4787440/150‑1 (MQ=255)

                                                                     gTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGcc                                                                               >  1:990781/1‑150 (MQ=255)

                                                                         tCCCAGGCGTTGATCTGGCCGGGACGGGCAATGGACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                                                                           <  2:2521583/150‑1 (MQ=255)

                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         <  2:800770/150‑1 (MQ=255)

                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         <  2:134835/150‑1 (MQ=255)

                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCACTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         >  2:4091009/1‑150 (MQ=255)

                                                                             aGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCa                                                                       >  1:2199837/1‑150 (MQ=255)

                                                                                  ttGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                             <  2:2844605/107‑1 (MQ=255)

                                                                                      tCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgt                                                              >  2:1051681/1‑150 (MQ=255)

                                                                                       cTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                               >  2:491493/1‑100 (MQ=255)

                                                                                       cTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                               <  1:491493/100‑1 (MQ=255)

                                                                                        tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                            >  2:5449777/1‑150 (MQ=255)

                                                                                        tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                            >  2:3431073/1‑150 (MQ=255)

                                                                                        tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                            <  1:3962818/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  2:1234531/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          >  2:730341/1‑150 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  1:2805356/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  1:297683/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  2:3223587/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  2:1132674/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  1:1017128/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  1:708007/150‑1 (MQ=255)

                                                                                          gCCGGGACGGGCAATGGACGGCGCATCCGGGACCATCTCGGTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  1:1698363/150‑1 (MQ=255)

                                                                                           ccGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                        >  1:1952522/1‑103 (MQ=255)

                                                                                           ccGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                        <  2:1952522/103‑1 (MQ=255)

                                                                                            cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                        >  1:111790/1‑150 (MQ=255)

                                                                                             gggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGaa                                                       <  1:201357/150‑1 (MQ=255)

                                                                                              ggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAAt                                                      <  1:2256594/150‑1 (MQ=255)

                                                                                               gACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAAtt                                                     <  2:2464487/150‑1 (MQ=255)

                                                                                                    gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                <  2:2680471/150‑1 (MQ=255)

                                                                                                    gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                <  2:3874589/150‑1 (MQ=255)

                                                                                                     cAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTgg                                               >  1:4871813/1‑150 (MQ=255)

                                                                                                            cGGCGCATCCGGGAACATCTCGTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGaaaa                                        <  1:2084709/150‑1 (MQ=255)

                                                                                                             ggCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGaaaaa                                       >  1:5088312/1‑150 (MQ=255)

                                                                                                              gcgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAAt                                      >  1:4158630/1‑150 (MQ=255)

                                                                                                                gcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATcc                                    <  1:1310776/150‑1 (MQ=255)

                                                                                                                  aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                  <  1:5257950/150‑1 (MQ=255)

                                                                                                                  aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                  <  2:5088312/150‑1 (MQ=255)

                                                                                                                        gAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                >  2:5136744/1‑130 (MQ=255)

                                                                                                                        gAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAg                            >  1:1218926/1‑150 (MQ=255)

                                                                                                                         aaCATCTCTGTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGc                           <  1:5181457/150‑1 (MQ=255)

                                                                                                                            aTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgag                        >  2:628390/1‑150 (MQ=255)

                                                                                                                             tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                       >  2:296778/1‑150 (MQ=255)

                                                                                                                               tctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATc                     >  1:1071165/1‑150 (MQ=255)

                                                                                                                                 tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                   >  2:2551121/1‑150 (MQ=255)

                                                                                                                                  gtCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGa                  <  1:3913593/149‑1 (MQ=255)

                                                                                                                                   tCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGAc                 <  1:4438152/150‑1 (MQ=255)

                                                                                                                                      gCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCgg              >  2:3680487/1‑150 (MQ=255)

                                                                                                                                       cTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGt             <  2:4436592/150‑1 (MQ=255)

                                                                                                                                             cACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtggtg       <  2:1494926/150‑1 (MQ=255)

                                                                                                                                               ccAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTggtggt     >  2:2746132/1‑150 (MQ=255)

                                                                                                                                                  gCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGa  >  1:4691132/1‑150 (MQ=255)

ATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGA  >  NC_002947/5986747‑5987042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`