BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,024,627	T→A	5.4%	V286E (GTG→GAG) ‡	PP_5273 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,024,627	0	T	A	5.4%	632.8 / 16.9	185	V286E (GTG→GAG) ‡	PP_5273	oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (74/101); new base A (6/4); total (80/105)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 186 total aligned reads displayed.

GTGCTGCAACGCCTACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTGCGC  >  NC_002947/6024478‑6024772

       aaCGCCTACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTg                                                                                                                                            <  1:467836/150‑1 (MQ=255)

         cGCCTACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGa                                                                                                                                          <  1:4482439/150‑1 (MQ=255)

         cGCCTACCACAACGACCTCAATCGCGAGCGGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGa                                                                                                                                          <  1:3523357/150‑1 (MQ=255)

            cTACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGacta                                                                                                                                       <  2:4382136/150‑1 (MQ=255)

             tACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGactac                                                                                                                                      >  2:4561710/1‑150 (MQ=255)

              aCCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGactact                                                                                                                                     >  1:5435502/1‑150 (MQ=255)

               ccACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGActacta                                                                                                                                    <  1:397780/150‑1 (MQ=255)

               ccACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGActacta                                                                                                                                    >  1:144500/1‑150 (MQ=255)

                cacaACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACtactac                                                                                                                                   <  2:5255916/150‑1 (MQ=255)

                cacaACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACtactac                                                                                                                                   <  1:3589450/150‑1 (MQ=255)

                  caACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACAATGCTGCCGCAGAATAAGGCGGTGTGTGACCAGCGCGTGGCGCAGGACTACTACCg                                                                                                                                 >  1:2022530/1‑150 (MQ=255)

                        ccTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTc                                                                                                                           >  2:2849220/1‑150 (MQ=255)

                          tCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTccg                                                                                                                         >  1:5099681/1‑150 (MQ=255)

                           cAATCGCGAGCTGGGCGGCAAGGTGAATCCGGCCGGCAGCTACATCATCGCCACCGAGCCGATGGGCGAAGAACGCGCACGCACATTGCTGCCGCATAATATGGCGGTTTGTGACCAGCGCGTGGCGCTGGACTACTACCGACTGTccgc                                                                                                                        >  1:1202136/1‑150 (MQ=255)

                            aaTCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTccgcc                                                                                                                       <  1:2383101/150‑1 (MQ=255)

                            aaTCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTccgcc                                                                                                                       <  2:1882504/150‑1 (MQ=255)

                            aaTCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTccgcc                                                                                                                       >  2:3705068/1‑150 (MQ=255)

                                      tGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGTCCAGCGCCACGCGCTggtca                                                                                                                                        <  2:3384804/123‑4 (MQ=255)

                                      tGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGTCCAGCGCCACGCGCTggtca                                                                                                                                        >  1:3384794/1‑120 (MQ=255)

                                      tGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGcc                                                                                                             >  2:3537209/1‑150 (MQ=255)

                                        ggcggcAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCctg                                                                                                           <  1:2303925/150‑1 (MQ=255)

                                        ggcggcAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCctg                                                                                                           <  1:1776918/150‑1 (MQ=255)

                                        ggcggcAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCctg                                                                                                           <  2:4310833/150‑1 (MQ=255)

                                           ggcAAGGTGCTGCCGGCCGGCAGCTACATCCTCGCCAGCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGACTATGGCGGGGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCcggctg                                                                                                        >  2:4704090/1‑150 (MQ=255)

                                              aaGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGttt                                                                                                     <  2:914707/150‑1 (MQ=255)

                                               aGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTg                                                                                                    <  1:1869131/150‑1 (MQ=255)

                                                gtagctgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTgg                                                                                                   >  1:2603760/4‑150 (MQ=255)

                                                 gTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGc                                                                                                  <  1:569976/150‑1 (MQ=255)

                                                  tgctgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCg                                                                                                 >  1:1030997/1‑150 (MQ=255)

                                                  tgctgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCg                                                                                                 <  2:2190027/150‑1 (MQ=255)

                                                   gctgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCgg                                                                                                >  2:4085304/1‑150 (MQ=255)

                                                     tgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCCCGCACATTGCTGCCGCAGAATATGGCGGTGTGTGTCCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtg                                                                                              <  1:360413/150‑1 (MQ=255)

                                                     tgcCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGGCTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtg                                                                                              <  1:360485/150‑1 (MQ=255)

                                                      gccggccgGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtgc                                                                                             >  2:4238764/1‑150 (MQ=255)

                                                      gccggccgGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtgc                                                                                             <  1:2066058/150‑1 (MQ=255)

                                                       ccggccggCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtgcc                                                                                            >  1:5268071/1‑150 (MQ=255)

                                                          gccggcAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtgcctgc                                                                                         >  2:1933113/1‑150 (MQ=255)

                                                          gccggcAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGtgcctgc                                                                                         <  1:2983578/150‑1 (MQ=255)

                                                                 gCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTAtt                                                                                  <  1:479386/150‑1 (MQ=255)

                                                                  cTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTc                                                                                 <  1:3855304/150‑1 (MQ=255)

                                                                   tACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCa                                                                                >  2:1824226/1‑150 (MQ=255)

                                                                   tACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCa                                                                                >  2:1805734/1‑150 (MQ=255)

                                                                   tACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCa                                                                                <  1:4622414/150‑1 (MQ=255)

                                                                    aCATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAg                                                                               <  1:1655990/150‑1 (MQ=255)

                                                                      atcatcGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGc                                                                             >  2:3493810/1‑150 (MQ=255)

                                                                       tcatcGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGcc                                                                            <  1:5030730/150‑1 (MQ=255)

                                                                            gCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGAc                                                                       <  2:1787481/150‑1 (MQ=255)

                                                                             ccACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGAGACTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGAcc                                                                      >  2:1283044/1‑150 (MQ=255)

                                                                               aCCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCg                                                                    >  1:3503350/1‑150 (MQ=255)

                                                                                 cGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGaa                                                                  <  2:107690/150‑1 (MQ=255)

                                                                                    gCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGa                                                               >  1:66596/1‑150 (MQ=255)

                                                                                     ccGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGAc                                                              <  1:5153509/150‑1 (MQ=255)

                                                                                      cGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGAc                                                                       >  2:4949333/1‑140 (MQ=255)

                                                                                      cGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGAc                                                                       <  1:4949333/140‑1 (MQ=255)

                                                                                      cGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGAc                                                                       >  2:4949237/1‑140 (MQ=255)

                                                                                           ggCGAAGAACGCGCACGCACATTGCTGCCGCAGACTATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATcgcc                                                        <  2:3563171/150‑1 (MQ=255)

                                                                                            gCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATcgccg                                                       <  2:1163920/150‑1 (MQ=255)

                                                                                            gCGAAGAACGCGCACGCACAGTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACGACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATcgccg                                                       <  2:4797976/150‑1 (MQ=255)

                                                                                              gaagaaCGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCg                                                                                                 <  1:4039661/106‑1 (MQ=255)

                                                                                              gaagaaCGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCg                                                                                                 >  2:4039661/1‑106 (MQ=255)

                                                                                                 gaaCGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTAc                                                  <  1:4568410/150‑1 (MQ=255)

                                                                                                 gaaCGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTAc                                                  <  1:4568613/150‑1 (MQ=255)

                                                                                                   aCGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACAt                                                >  1:4270056/1‑150 (MQ=255)

                                                                                                    cgcgCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATg                                               >  2:3048126/1‑150 (MQ=255)

                                                                                                       gcacgcacATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTCTTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCgg                                            >  1:2709857/1‑150 (MQ=255)

                                                                                                       gcacgcacATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCgg                                            <  1:3177815/150‑1 (MQ=255)

                                                                                                       gcacgcacATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCgg                                            <  2:2971729/150‑1 (MQ=255)

                                                                                                         acgcacAGTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGcc                                          <  1:1029875/150‑1 (MQ=255)

                                                                                                           gcacATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGaccac                                                                                                                 <  1:3194933/77‑1 (MQ=255)

                                                                                                           gcacATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGaccac                                                                                                                 >  2:3194933/1‑77 (MQ=255)

                                                                                                            cacaTTGCTGCCGCAGAGTATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGaa                                       <  1:2637153/150‑1 (MQ=255)

                                                                                                             acaTTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAg                                      <  2:1559158/150‑1 (MQ=255)

                                                                                                             acaTTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAg                                      >  1:2323062/1‑150 (MQ=255)

                                                                                                              caTTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGa                                     <  2:3302323/150‑1 (MQ=255)

                                                                                                               aTTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGAt                                    >  2:2480783/1‑150 (MQ=255)

                                                                                                                     gcCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCaa                              <  2:3511332/150‑1 (MQ=255)

                                                                                                                      ccGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAg                             >  1:421144/1‑150 (MQ=255)

                                                                                                                        gCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGgt                           >  2:5380058/1‑150 (MQ=255)

                                                                                                                        gCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGgt                           >  1:4513610/1‑150 (MQ=255)

                                                                                                                         cAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGgtg                          >  1:3748978/1‑150 (MQ=255)

                                                                                                                            aaTATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTc                       >  1:1582530/1‑150 (MQ=255)

                                                                                                                              tatGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTccc                     <  1:3264395/150‑1 (MQ=255)

                                                                                                                              tatGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTccc                     <  1:162689/150‑1 (MQ=255)

                                                                                                                                      tgtgtgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTg             >  2:2650092/1‑150 (MQ=255)

                                                                                                                                       gtgtgACCAGTGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGc            <  2:880574/150‑1 (MQ=255)

                                                                                                                                       gtgtgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGc            <  1:5360096/150‑1 (MQ=255)

                                                                                                                                       gtgtgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGc            <  1:1266588/150‑1 (MQ=255)

                                                                                                                                        tgtgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGcc           >  2:2443463/1‑150 (MQ=255)

                                                                                                                                         tagtccAGCGCCACGCGCTGGTCTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCg          >  1:916557/5‑150 (MQ=255)

                                                                                                                                          tgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGa         >  2:4355415/1‑150 (MQ=255)

                                                                                                                                          tgACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGa         >  1:4376036/1‑150 (MQ=255)

                                                                                                                                            tccAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACg       >  2:1402217/2‑150 (MQ=255)

                                                                                                                                            tccAGCGCCACGCGCTGGTCTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATc                                                           <  2:772639/97‑1 (MQ=255)

                                                                                                                                            tccAGCGCCACGCGCTGGTCTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATc                                                           >  1:772639/2‑98 (MQ=255)

                                                                                                                                            aCCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACg       >  2:601777/1‑150 (MQ=255)

                                                                                                                                               aGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTgc    >  1:4248733/1‑150 (MQ=255)

                                                                                                                                               aGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTgc    >  1:888458/1‑150 (MQ=255)

                                                                                                                                                gcgcGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTgcg   >  2:1053373/1‑150 (MQ=255)

                                                                                                                                                gcgcGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTgcg   >  1:286832/1‑150 (MQ=255)

                                                                                                                                                 cgcgTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTgcgc  <  1:1385837/150‑1 (MQ=255)

GTGCTGCAACGCCTACCACAACGACCTCAATCGCGAGCTGGGCGGCAAGGTGCTGCCGGCCGGCAGCTACATCATCGCCACCGAGCCGCTGGGCGAAGAACGCGCACGCACATTGCTGCCGCAGAATATGGCGGTGTGTGACCAGCGCGTGGCGCTGGACTACTACCGCCTGTCCGCCGACCACCGCCTGCTGTTTGGCGGTGCCTGCCACTATTCAGGCCGCGACCCGAAGGACATCGCCGCCTACATGCGGCCGAAGATGCTCAAGGTGTTCCCGCAGCTTGCCGACGTGCGC  >  NC_002947/6024478‑6024772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`