BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,282	C→T	5.3%	noncoding (179/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,282	0	C	T	5.3%	414.9 / 7.9	132	noncoding (179/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand): ref base C (60/65); new base T (2/5); total (62/70)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 132 total aligned reads displayed.

TTGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGG  >  NC_002947/536134‑536428

ttGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                   <  1:1261605/150‑1 (MQ=255)

     gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcgc                                                                                                                                              >  1:2580913/1‑150 (MQ=255)

     gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcgc                                                                                                                                              >  1:2269536/1‑150 (MQ=255)

       ccTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCgcgc                                                                                                                                            >  1:4602711/1‑150 (MQ=255)

        cTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgcc                                                                                                                                           <  1:602698/150‑1 (MQ=255)

        cTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgcc                                                                                                                                           <  1:1298684/150‑1 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgccg                                                                                                                                          <  1:306945/150‑1 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgccg                                                                                                                                          <  1:306944/150‑1 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                          >  2:904515/1‑150 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                          >  2:83983/1‑150 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                          >  2:3022740/1‑150 (MQ=255)

          gATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGGTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccgc                                                                                                                                         <  2:1203522/150‑1 (MQ=255)

            tCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCa                                                                                                                                       <  1:5112012/150‑1 (MQ=255)

              gCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGCGCCGCCatg                                                                                                                                     <  1:721430/150‑3 (MQ=255)

                    cTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAt                                                                                                                               >  2:1505941/1‑150 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                   >  1:4291579/1‑129 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                   <  2:4291579/129‑1 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAtt                                                                                                                              >  2:3148153/1‑150 (MQ=255)

                       aaGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTgg                                                                                                                            <  1:3105550/150‑1 (MQ=255)

                        aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTggg                                                                                                                           <  2:4861958/150‑1 (MQ=255)

                        aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTggg                                                                                                                           <  2:470269/150‑1 (MQ=255)

                        aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTggg                                                                                                                           <  2:4695130/150‑1 (MQ=255)

                           gTATCAAGCCAGCTTCCACGAAAGGTATCTACGTTAAGCGCGTTACACTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTACGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTc                                                                                                                        >  1:1877220/1‑150 (MQ=255)

                              acAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcct                                                                                                                     <  1:4375820/149‑1 (MQ=255)

                                aaGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgc                                                                                                                   >  2:1558248/1‑150 (MQ=255)

                                  gCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgcct                                                                                                                 >  2:3553060/1‑150 (MQ=255)

                                   ccAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgcctg                                                                                                                >  1:7808/1‑150 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                          <  1:4840482/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                          <  2:1929694/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                          <  2:3451527/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGACGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                          <  2:507687/150‑1 (MQ=255)

                                          ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                         <  1:2971340/150‑1 (MQ=255)

                                          ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                         <  1:3232858/150‑1 (MQ=255)

                                            tCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCt                                                                                                       >  1:2683266/1‑150 (MQ=255)

                                                aaGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                                   >  2:1174924/1‑150 (MQ=255)

                                                  tgTATCTACGTTAATCGCGTTACACTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCATAGCTCGCTGAACGTGTAAGAACATTGCGGCTCGACCAGTCGCGCCGACATCAAAAATTTGTGTCCCTTCCTGTAGGGGGCTATCaaa                                                                                                 <  2:781819/149‑1 (MQ=255)

                                                    tATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGa                                                                                               <  2:239345/150‑1 (MQ=255)

                                                      tCTACGTTAAGCGAGTAACCCTGAGCACCACCATGGGCCCAGGTCAGGTCATCGAACAGAGCACGCTGAACGTGAAAGAACAAGGCGGCGCGCCCCGTCGCGCCGCCAGAAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGAcc                                                                                             >  1:4868921/1‑150 (MQ=255)

                                                             tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcg                                                                                      <  2:3592079/150‑1 (MQ=255)

                                                             tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcg                                                                                      <  2:4111288/150‑1 (MQ=255)

                                                                gcgcGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                                   >  1:425497/1‑150 (MQ=255)

                                                                gcgcGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                                   >  2:1632089/1‑150 (MQ=255)

                                                                gcgcGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                                   >  2:1631867/1‑150 (MQ=255)

                                                                   cgTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCaa                                                                                <  1:1365061/150‑1 (MQ=255)

                                                                    gTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAg                                                                               <  2:3483668/150‑1 (MQ=255)

                                                                    gTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAg                                                                               >  2:5360699/1‑150 (MQ=255)

                                                                     ttACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGc                                                                              <  2:2995984/150‑1 (MQ=255)

                                                                         ccTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCACAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTa                                                                          >  1:5033769/1‑150 (MQ=255)

                                                                          cTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCCGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTaa                                                                         <  2:5187061/150‑1 (MQ=255)

                                                                               caccacCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACAt                                                                    <  2:3163520/150‑1 (MQ=255)

                                                                               caccacCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACAt                                                                    >  2:2235293/1‑150 (MQ=255)

                                                                                  caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAg                                                                 >  1:2455261/1‑150 (MQ=255)

                                                                                     caTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGccc                                                              <  2:425497/150‑1 (MQ=255)

                                                                                                gTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGt                                                   >  2:204581/1‑150 (MQ=255)

                                                                                                  cTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGc                                                 <  2:4602711/150‑1 (MQ=255)

                                                                                                    ggTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTc                                               <  1:1279240/150‑1 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGc                                                 <  2:3178232/147‑1 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGc                                                 >  1:3178232/1‑147 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTcc                                              <  1:1558248/150‑1 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTcc                                              >  1:3867379/1‑150 (MQ=255)

                                                                                                      tCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                             >  2:675336/1‑150 (MQ=255)

                                                                                                        aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGa                                           >  1:2035892/1‑150 (MQ=255)

                                                                                                         tCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGAt                                          >  1:406344/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                        <  1:5210952/150‑1 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                        >  2:4543195/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                        >  1:531405/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                        <  1:331816/150‑1 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                        >  1:580198/1‑150 (MQ=255)

                                                                                                            aTCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCg                                       <  2:2476370/150‑1 (MQ=255)

                                                                                                            aTCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCg                                       >  1:3266265/1‑150 (MQ=255)

                                                                                                               agagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                    <  1:481659/150‑1 (MQ=255)

                                                                                                                gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                                   <  1:777961/150‑1 (MQ=255)

                                                                                                                gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                                   <  1:4866698/150‑1 (MQ=255)

                                                                                                                 agCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAcc                                  <  2:1211992/150‑1 (MQ=255)

                                                                                                                     cGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAAt                              <  1:1785823/150‑1 (MQ=255)

                                                                                                                      gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATc                             <  2:5070639/150‑1 (MQ=255)

                                                                                                                      gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATc                             >  2:1693831/1‑150 (MQ=255)

                                                                                                                         gAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGa                          <  1:12563/150‑1 (MQ=255)

                                                                                                                          aaCGTGTAAGACCATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAgga                         <  2:2219327/150‑3 (MQ=255)

                                                                                                                          aaCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCTGATTCGGTAACGAATcggga                         <  1:1505941/150‑5 (MQ=255)

                                                                                                                           aCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGaca                        <  1:4577949/150‑1 (MQ=255)

                                                                                                                             gtgtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACAcc                      >  2:2169517/1‑150 (MQ=255)

                                                                                                                              tgtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCa                     <  2:4608439/150‑1 (MQ=255)

                                                                                                                              tgtAAGAACATGGCGGCGCGACCAGTCCCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCACCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCa                     <  2:4608489/150‑1 (MQ=255)

                                                                                                                               gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaa                    >  1:3238461/1‑150 (MQ=255)

                                                                                                                                tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaa                   >  2:1638440/1‑150 (MQ=255)

                                                                                                                                tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaa                   >  1:5416119/1‑150 (MQ=255)

                                                                                                                                 tttttgctGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaaa                  >  1:4853414/8‑150 (MQ=255)

                                                                                                                                       aTGGCGGCGCGACCACTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTACGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACAt            <  2:2580913/150‑1 (MQ=255)

                                                                                                                                          gcggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCg         >  2:604514/1‑150 (MQ=255)

                                                                                                                                           cggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCgg        >  1:4262202/1‑150 (MQ=255)

                                                                                                                                            ggcgCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTaa                                                                         <  1:987007/84‑1 (MQ=255)

                                                                                                                                            ggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGc       >  1:3569253/1‑150 (MQ=255)

                                                                                                                                            ggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGc       >  2:3944734/1‑150 (MQ=255)

                                                                                                                                             gcgcGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc      >  2:1404145/1‑150 (MQ=255)

                                                                                                                                             gcgcGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc      >  1:3444912/1‑150 (MQ=255)

                                                                                                                                             gcgcGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc      >  2:2827544/1‑150 (MQ=255)

                                                                                                                                               gcgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTc    >  1:2299462/1‑150 (MQ=255)

                                                                                                                                                  aCCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                    <  2:4035860/115‑1 (MQ=255)

                                                                                                                                                  aCCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                    >  1:4035860/1‑115 (MQ=255)

TTGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGG  >  NC_002947/536134‑536428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`