BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,283	A→G	5.4%	noncoding (178/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,283	0	A	G	5.4%	407.2 / 8.1	129	noncoding (178/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand): ref base A (58/63); new base G (2/5); total (61/68)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 134 total aligned reads displayed.

TTGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAG  >  NC_002947/536134‑536432

ttGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                       <  1:1261605/150‑1 (MQ=255)

   aaGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTc                                                                                                                                                    <  2:4868117/150‑1 (MQ=255)

     gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcgc                                                                                                                                                  >  1:2580913/1‑150 (MQ=255)

     gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcgc                                                                                                                                                  >  1:2269536/1‑150 (MQ=255)

       ccTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCgcgc                                                                                                                                                >  1:4602711/1‑150 (MQ=255)

        cTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgcc                                                                                                                                               <  1:602698/150‑1 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgccg                                                                                                                                              <  1:306945/150‑1 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                              >  2:904515/1‑150 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                              >  2:83983/1‑150 (MQ=255)

         tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccg                                                                                                                                              >  2:3022740/1‑150 (MQ=255)

            tCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCa                                                                                                                                           <  1:5112012/150‑1 (MQ=255)

              gCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGCGCCGCCatg                                                                                                                                         <  1:721430/150‑3 (MQ=255)

              gCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGc                                                                                                                                         >  2:3105550/1‑150 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                       <  2:4291579/129‑1 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCa                                                                                                                                                       >  1:4291579/1‑129 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAtt                                                                                                                                  <  2:2115985/150‑1 (MQ=255)

                     tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAtt                                                                                                                                  >  2:3148153/1‑150 (MQ=255)

                       aaGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTgg                                                                                                                                <  1:3105550/150‑1 (MQ=255)

                        aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTggg                                                                                                                               <  2:4861958/150‑1 (MQ=255)

                        aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTggg                                                                                                                               <  2:470269/150‑1 (MQ=255)

                           gTATCAAGCCAGCTTCCACGAAAGGTATCTACGTTAAGCGCGTTACACTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTACGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTc                                                                                                                            >  1:1877220/1‑150 (MQ=255)

                              acAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcct                                                                                                                         <  1:4375820/149‑1 (MQ=255)

                                aaGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgc                                                                                                                       >  2:1558248/1‑150 (MQ=255)

                                  gCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgcct                                                                                                                     >  2:3553060/1‑150 (MQ=255)

                                       cTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgg                                                                                                                >  1:1670611/1‑150 (MQ=255)

                                        ttCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggg                                                                                                               <  1:3060137/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                              <  1:4840482/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                              <  2:3451527/150‑1 (MQ=255)

                                         tCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGACGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                              <  2:507687/150‑1 (MQ=255)

                                          ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                             <  1:2971340/150‑1 (MQ=255)

                                          ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                             <  1:2550810/150‑1 (MQ=255)

                                          ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                             <  1:3232858/150‑1 (MQ=255)

                                            tCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCt                                                                                                           >  1:2683266/1‑150 (MQ=255)

                                              gAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTAt                                                                                                         >  2:276501/1‑150 (MQ=255)

                                                aaGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                                       >  2:1174924/1‑150 (MQ=255)

                                                 aGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCa                                                                                                      <  2:466563/150‑1 (MQ=255)

                                                    tATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGa                                                                                                   <  2:239345/150‑1 (MQ=255)

                                                      tCTACGTTAAGCGAGTAACCCTGAGCACCACCATGGGCCCAGGTCAGGTCATCGAACAGAGCACGCTGAACGTGAAAGAACAAGGCGGCGCGCCCCGTCGCGCCGCCAGAAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGAcc                                                                                                 >  1:4868921/1‑150 (MQ=255)

                                                         aCGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTa                                                                                              >  1:1314124/1‑150 (MQ=255)

                                                             tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcg                                                                                          <  2:3592079/150‑1 (MQ=255)

                                                             tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcg                                                                                          <  2:4111288/150‑1 (MQ=255)

                                                                gcgcGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                                       >  2:1631867/1‑150 (MQ=255)

                                                                gcgcGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                                       >  1:425497/1‑150 (MQ=255)

                                                                   cgTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCaa                                                                                    <  1:1365061/150‑1 (MQ=255)

                                                                         ccTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCACAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTa                                                                              >  1:5033769/1‑150 (MQ=255)

                                                                          cTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCCGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTaa                                                                             <  2:5187061/150‑1 (MQ=255)

                                                                               caccacCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACAt                                                                        >  2:2235293/1‑150 (MQ=255)

                                                                                  caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAg                                                                     >  2:15963/1‑150 (MQ=255)

                                                                                    ccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCt                                                                                <  2:711515/137‑1 (MQ=255)

                                                                                           cccAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAg                                                            >  2:1279240/1‑150 (MQ=255)

                                                                                                gTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGt                                                       >  2:204581/1‑150 (MQ=255)

                                                                                                    ggTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTc                                                   <  1:1279240/150‑1 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGc                                                     <  2:3178232/147‑1 (MQ=255)

                                                                                                     gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTcc                                                  >  1:3867379/1‑150 (MQ=255)

                                                                                                      tCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                                 >  2:675336/1‑150 (MQ=255)

                                                                                                         tCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGAt                                              >  1:406344/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            <  1:331816/150‑1 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            <  2:531405/150‑1 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            >  1:580198/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            >  1:531405/1‑150 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            <  1:5210952/150‑1 (MQ=255)

                                                                                                           gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                            >  2:4543195/1‑150 (MQ=255)

                                                                                                               agagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                        <  1:481659/150‑1 (MQ=255)

                                                                                                                gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                                       <  1:777961/150‑1 (MQ=255)

                                                                                                                gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                                       <  1:4866698/150‑1 (MQ=255)

                                                                                                                 agCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAcc                                      <  1:3514636/150‑1 (MQ=255)

                                                                                                                 agCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAcc                                      <  2:1211992/150‑1 (MQ=255)

                                                                                                                     cGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAAt                                  <  1:1785823/150‑1 (MQ=255)

                                                                                                                      gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATc                                 >  2:1693831/1‑150 (MQ=255)

                                                                                                                         gAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCTGATTCGGTAACGAATcggg                              <  1:204581/150‑4 (MQ=255)

                                                                                                                         gAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGa                              <  1:12563/150‑1 (MQ=255)

                                                                                                                          aaCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCTGATTCGGTAACGAATcggga                             <  1:813226/150‑5 (MQ=255)

                                                                                                                          aaCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCTGATTCGGTAACGAATcggga                             <  1:1505941/150‑5 (MQ=255)

                                                                                                                           aCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGaca                            <  1:4577949/150‑1 (MQ=255)

                                                                                                                             gtgtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACAcc                          >  2:2169517/1‑150 (MQ=255)

                                                                                                                              tgtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCa                         <  2:4608439/150‑1 (MQ=255)

                                                                                                                              tgtAAGAACATGGCGGCGCGACCAGTCCCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCACCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCa                         <  2:4608489/150‑1 (MQ=255)

                                                                                                                               gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaa                        >  1:3238461/1‑150 (MQ=255)

                                                                                                                                tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaa                       >  2:1638440/1‑150 (MQ=255)

                                                                                                                                tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaa                       >  1:5416119/1‑150 (MQ=255)

                                                                                                                                 tttttgctGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaaa                      >  1:4853414/8‑150 (MQ=255)

                                                                                                                                 aaGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaaaa                      <  1:3148153/150‑1 (MQ=255)

                                                                                                                                  agtagaTGGCGGCGCGTCCAGTCTCTCCGCCAGAAAAAATGTGGGTCCCTGCCTGGCGGGGGCTTTCGAAGACCGTAGGCGGATCTAGCCTTAAAACATCAGCCCCCGCTGATTGTGCTCCCGATTCGTTACCGAATCAGAGTCGAAAAc                     <  2:3798558/145‑1 (MQ=255)

                                                                                                                                  aGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcggc                                                                                        <  2:3247050/83‑1 (MQ=255)

                                                                                                                                  aGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcggc                                                                                        >  1:3247050/1‑83 (MQ=255)

                                                                                                                                       aTGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACAt                <  2:2269536/150‑1 (MQ=255)

                                                                                                                                       aTGGCGGCGCGACCACTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTACGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACAt                <  2:2580913/150‑1 (MQ=255)

                                                                                                                                          gcggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCg             >  2:604514/1‑150 (MQ=255)

                                                                                                                                           cggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCgg            >  1:4262202/1‑150 (MQ=255)

                                                                                                                                            ggcgCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTaa                                                                             <  1:987007/84‑1 (MQ=255)

                                                                                                                                             gcgcGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc          >  2:1404145/1‑150 (MQ=255)

                                                                                                                                             gcgcGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc          >  2:2827544/1‑150 (MQ=255)

                                                                                                                                             gcgcGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGcc          >  1:3444912/1‑150 (MQ=255)

                                                                                                                                               gcgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTc        >  1:2299462/1‑150 (MQ=255)

                                                                                                                                               gcgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTGAAAACATCAGCCCACGCAGATGGTGCTCCCGATGCGTTCCCGAATCAGACACCAAAACGACAGCCGGCCTc        >  2:4572615/1‑150 (MQ=255)

                                                                                                                                                cgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCg       <  2:4244345/150‑1 (MQ=255)

                                                                                                                                                 gACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCgg      <  2:1670611/150‑1 (MQ=255)

                                                                                                                                                  aCCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                        <  2:4035860/115‑1 (MQ=255)

                                                                                                                                                  aCCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                        >  1:4035860/1‑115 (MQ=255)

                                                                                                                                                     aGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAg  >  1:2956884/1‑150 (MQ=255)

TTGAAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAG  >  NC_002947/536134‑536432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`