BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,802	T→C	6.9%	R491R (CGT→CGC)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,802	0	T	C	6.9%	495.8 / 16.8	160	R491R (CGT→CGC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base T (76/73); new base C (6/5); total (82/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 164 total aligned reads displayed.

ACCCAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAC  >  NC_002947/14653‑14950

aCCCAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGt                                                                                                                                                      <  2:616737/150‑1 (MQ=255)

aCCCAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGt                                                                                                                                                      <  1:982191/150‑1 (MQ=255)

aCCCAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGt                                                                                                                                                      <  1:3971861/150‑1 (MQ=255)

 cccAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGtt                                                                                                                                                     >  1:1397623/1‑150 (MQ=255)

     ggCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATca                                                                                                                                                 <  1:1119520/150‑1 (MQ=255)

            ttGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatc                                                                                                                                          <  1:2316739/150‑1 (MQ=255)

            ttGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatc                                                                                                                                          >  1:1296730/1‑150 (MQ=255)

             tGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatca                                                                                                                                         <  1:534061/150‑1 (MQ=255)

             tGCCGCTGAAGGGT‑‑AATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatc                                                                                                                                       <  1:4369293/150‑1 (MQ=255)

               ccGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatc                                                                                                                                       <  1:4755649/150‑1 (MQ=255)

               ccGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatc                                                                                                                                       <  1:5434795/150‑1 (MQ=255)

                cGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatca                                                                                                                                      >  2:5444991/1‑150 (MQ=255)

                cGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatca                                                                                                                                      >  1:1464143/1‑150 (MQ=255)

                cGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatca                                                                                                                                      >  1:1464175/1‑150 (MQ=255)

                  cTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatcatc                                                                                                                                    <  1:2428594/150‑1 (MQ=255)

                      aGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGa                                                                                                                                <  1:177223/150‑1 (MQ=255)

                       gggTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGAc                                                                                                                               <  1:937630/150‑1 (MQ=255)

                        ggTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGAcc                                                                                                                              >  2:5435134/1‑150 (MQ=255)

                         gTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCg                                                                                                                             >  1:1415533/1‑150 (MQ=255)

                              aTCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCt                                                                                                                        <  1:1855935/150‑1 (MQ=255)

                              aTCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCt                                                                                                                        <  1:2482619/150‑1 (MQ=255)

                                ccTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGa                                                                                                                      <  2:992347/150‑1 (MQ=255)

                                ccTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGa                                                                                                                      <  1:3776905/150‑1 (MQ=255)

                                 cTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGAc                                                                                                                     >  1:3439220/1‑150 (MQ=255)

                                 cTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGAc                                                                                                                     >  1:274331/1‑150 (MQ=255)

                                       gTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGAc                                                                                                               >  1:3941915/1‑150 (MQ=255)

                                          gagaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGt                                                                                                            >  1:3474904/1‑150 (MQ=255)

                                            gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAAcatcatc                                                                                                                                       <  2:1409472/121‑1 (MQ=255)

                                            gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTc                                                                                                          >  1:1279096/1‑150 (MQ=255)

                                            gaAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTc                                                                                                          >  1:3791442/1‑150 (MQ=255)

                                               aGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGca                                                                                                       <  2:2809217/150‑1 (MQ=255)

                                                          aCAAGATGATTTCGTCCCAGGCAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgc                                                                                            <  2:4454696/150‑1 (MQ=255)

                                                            aaGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctg                                                                                          >  1:1372090/1‑150 (MQ=255)

                                                            aaGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctg                                                                                          >  2:1867856/1‑150 (MQ=255)

                                                             aGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgc                                                                                         <  1:1016763/150‑1 (MQ=255)

                                                              gatgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgct                                                                                        >  2:1842440/1‑150 (MQ=255)

                                                               atgatTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctgctg                                                                                       <  2:1397623/150‑1 (MQ=255)

                                                                  atTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctg                                                                                    <  2:2518490/150‑1 (MQ=255)

                                                                  atTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctg                                                                                    <  2:4332032/150‑1 (MQ=255)

                                                                   tttCGTGCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGTTGCTGACGTTGACGGTTCGCACATCCGTACGctgctgctgt                                                                                   >  2:4225409/1‑149 (MQ=255)

                                                                     tCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAcc                                                                                 <  2:5359691/150‑1 (MQ=255)

                                                                     tCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAcc                                                                                 <  2:4126942/150‑1 (MQ=255)

                                                                       gTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCg                                                                                                         <  2:5207845/124‑1 (MQ=255)

                                                                       gTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCg                                                                                                         <  2:5207821/124‑1 (MQ=255)

                                                                       gTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCg                                                                                                         >  1:5207821/1‑124 (MQ=255)

                                                                        tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                              >  2:2637084/1‑150 (MQ=255)

                                                                        tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                              >  1:2789470/1‑150 (MQ=255)

                                                                        tCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttc                                                                              <  2:3225320/150‑1 (MQ=255)

                                                                         cccAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttct                                                                             >  2:2713375/1‑150 (MQ=255)

                                                                           cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTGGCACATCCGTACGCTGCTGCTGACcttcttc                                                                           >  1:526896/1‑150 (MQ=255)

                                                                           cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttc                                                                           >  1:5279753/1‑150 (MQ=255)

                                                                           cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttc                                                                           <  2:1622723/150‑1 (MQ=255)

                                                                           cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttc                                                                           >  2:1508265/1‑150 (MQ=255)

                                                                           cAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttc                                                                           >  1:4857000/1‑150 (MQ=255)

                                                                             ggAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACcttcttctt                                                                         >  1:4844469/1‑150 (MQ=255)

                                                                               aaGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTcc                                                                       <  1:1686659/150‑1 (MQ=255)

                                                                                         gCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGcc                                                             >  2:911192/1‑150 (MQ=255)

                                                                                           tGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCgg                                                           >  2:5320058/1‑150 (MQ=255)

                                                                                                aCTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTg                                                      <  1:4020142/150‑1 (MQ=255)

                                                                                                aCTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTg                                                      <  1:5320011/150‑1 (MQ=255)

                                                                                                 cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                     <  2:1083685/150‑1 (MQ=255)

                                                                                                  tGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGt                                                    <  2:4277149/150‑1 (MQ=255)

                                                                                                  tGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGt                                                    >  2:4757023/1‑150 (MQ=255)

                                                                                                  tGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACAGCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGt                                                    <  2:4651641/150‑1 (MQ=255)

                                                                                                   gcgcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTc                                                   <  1:4171334/150‑1 (MQ=255)

                                                                                                     gcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGa                                                 <  2:1851896/150‑1 (MQ=255)

                                                                                                       tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                               >  2:4043764/1‑150 (MQ=255)

                                                                                                          gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                            <  1:841301/150‑1 (MQ=255)

                                                                                                           cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGGTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                           <  2:5284081/150‑1 (MQ=255)

                                                                                                            tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                          >  1:1515227/1‑150 (MQ=255)

                                                                                                                gCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACa                                      >  2:906257/1‑150 (MQ=255)

                                                                                                                 cATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACAt                                     <  1:709735/150‑1 (MQ=255)

                                                                                                                  aTCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctg                                                                                          <  2:306376/96‑1 (MQ=255)

                                                                                                                  aTCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACgctg                                                                                          >  1:306376/1‑96 (MQ=255)

                                                                                                                   tCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCt                                   <  1:911192/150‑1 (MQ=255)

                                                                                                                           gAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGcc                           <  1:4282179/150‑1 (MQ=255)

                                                                                                                            aaGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                          >  1:4881913/1‑150 (MQ=255)

                                                                                                                            aaGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                          >  1:4161503/1‑150 (MQ=255)

                                                                                                                             agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                         <  2:4857000/150‑1 (MQ=255)

                                                                                                                               agTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgc                       <  2:1296730/150‑1 (MQ=255)

                                                                                                                                 tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATc                                                                                                   >  2:369390/1‑72 (MQ=255)

                                                                                                                                 tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATc                                                                                                   <  1:369390/72‑1 (MQ=255)

                                                                                                                                  acaacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccgc                    >  2:3788120/1‑150 (MQ=255)

                                                                                                                                    aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                   <  1:1512674/85‑1 (MQ=255)

                                                                                                                                    aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                   >  2:1513135/1‑85 (MQ=255)

                                                                                                                                    aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGa                                                                                   >  2:1512674/1‑85 (MQ=255)

                                                                                                                                    aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                  >  1:2076839/1‑150 (MQ=255)

                                                                                                                                      tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCGGGAACTGATCGAGCGTGGCCATATCTATATCGCGCCGCCGCCGCt                >  1:4153222/5‑150 (MQ=255)

                                                                                                                                      tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                >  1:658744/5‑150 (MQ=255)

                                                                                                                                       cctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCtc               >  1:2877393/4‑149 (MQ=255)

                                                                                                                                       aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg               >  1:3657806/1‑150 (MQ=255)

                                                                                                                                       aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg               >  1:4110836/1‑150 (MQ=255)

                                                                                                                                        tCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGt              <  2:172909/150‑1 (MQ=255)

                                                                                                                                          gACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGACCTGATCGAGCGTGGCCAGATCTATATCGCGCAGCCGCCGCtctct            >  2:4825144/1‑146 (MQ=255)

                                                                                                                                          gACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTat            >  2:3955712/1‑149 (MQ=255)

                                                                                                                                            cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa          <  2:681771/150‑1 (MQ=255)

                                                                                                                                            cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa          <  2:1095161/150‑1 (MQ=255)

                                                                                                                                            cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa          <  2:3529576/150‑1 (MQ=255)

                                                                                                                                              aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg        <  1:4764406/150‑1 (MQ=255)

                                                                                                                                                    gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc  >  1:1379513/1‑150 (MQ=255)

ACCCAGGCGATCTTGCCGCTGAAGGGTAAAATCCTCAACGTCGAGAAAGCGCGCTTCGACAAGATGATTTCGTCCCAGGAAGTGGGCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAC  >  NC_002947/14653‑14950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`