BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	549,652	C→A	8.8%	A108A (GCC→GCA)	tufB →	elongation factor Tu‑B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	549,652	0	C	A	8.8%	560.0 / 34.2	181	A108A (GCC→GCA)	tufB	elongation factor Tu‑B
Reads supporting (aligned to +/- strand): ref base C (75/90); new base A (4/12); total (79/102)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 184 total aligned reads displayed.

CGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGC  >  NC_002947/549505‑549800

cgGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGgccgc                                                                                                                                                    <  1:1962623/150‑1 (MQ=32)

   tATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGa                                                                                                                                                 <  2:4924731/150‑1 (MQ=32)

     tCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATg                                                                                                                                               <  2:3107981/150‑1 (MQ=32)

       aCCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACGTGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGt                                                                                                                                             >  1:5383447/1‑150 (MQ=17)

        ccATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTc                                                                                                                                            <  2:2122138/150‑1 (MQ=32)

         cATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTcc                                                                                                                                           >  2:5299957/1‑150 (MQ=32)

         cATCAACACCGCTCACGTCGAGTACAACTAGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTcc                                                                                                                                           >  2:1097917/1‑150 (MQ=17)

           tCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGa                                                                                                                                         >  2:1638368/1‑150 (MQ=32)

              acacCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGc                                                                                                                                      >  1:2203301/1‑150 (MQ=32)

               cacCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGcc                                                                                                                                     <  2:2400687/150‑1 (MQ=32)

               cacCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGcc                                                                                                                                     >  1:350656/1‑150 (MQ=32)

                acCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCg                                                                                                                                    >  1:3253137/1‑150 (MQ=32)

                       aCGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAAccc                                                                                                                             >  1:2532607/1‑150 (MQ=34)

                         gTCGAGTACAACTCGAACATTCGTCACTACGCTCATGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGt                                                                                                                           >  1:2850856/1‑150 (MQ=21)

                         gTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGt                                                                                                                           <  2:2223662/150‑1 (MQ=34)

                           cGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGa                                                                                                                         <  2:1781631/150‑1 (MQ=34)

                           cGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGa                                                                                                                         <  2:647421/150‑1 (MQ=34)

                           cGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGa                                                                                                                         <  2:667593/150‑1 (MQ=34)

                           cGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGa                                                                                                                         <  1:604523/150‑1 (MQ=34)

                            gAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAg                                                                                                                        >  1:5295006/1‑150 (MQ=34)

                                 cAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACAt                                                                                                                   <  2:1194640/150‑1 (MQ=34)

                                  aaCTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATc                                                                                                                  <  2:824375/150‑1 (MQ=34)

                                  aaCTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATc                                                                                                                  <  2:57429/150‑1 (MQ=34)

                                  aaCTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATc                                                                                                                  >  2:591708/1‑150 (MQ=34)

                                     tCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctt                                                                                                               <  2:704934/150‑2 (MQ=21)

                                     tCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctg                                                                                                               >  1:5101423/1‑150 (MQ=34)

                                       gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                             >  1:354795/1‑150 (MQ=35)

                                       gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                             >  1:354782/1‑150 (MQ=35)

                                       gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                             <  2:1529482/150‑1 (MQ=35)

                                            ttCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTccc                                                                                                        <  1:2749112/150‑1 (MQ=34)

                                               gTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTc                                                                                                     >  1:2301326/1‑150 (MQ=34)

                                               gTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTc                                                                                                     >  1:315105/1‑150 (MQ=34)

                                                   cTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGt                                                                                                 >  2:2672153/1‑150 (MQ=34)

                                                    tACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTa                                                                                                <  1:5097753/150‑1 (MQ=34)

                                                         tCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGt                                                                                           >  1:3638470/1‑150 (MQ=34)

                                                         tCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGt                                                                                           >  1:1337406/1‑150 (MQ=34)

                                                            cGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTcc                                                                                        >  1:3577833/1‑150 (MQ=34)

                                                            cGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTcc                                                                                        <  1:551048/150‑1 (MQ=34)

                                                             gTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCg                                                                                       <  2:2906263/150‑1 (MQ=34)

                                                               tGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTa                                                                                     <  2:5295006/150‑1 (MQ=34)

                                                                gACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTAc                                                                                    >  1:2359316/1‑150 (MQ=34)

                                                                 aCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACa                                                                                   >  2:1363742/1‑150 (MQ=34)

                                                                   tGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATc                                                                                 >  1:3312396/1‑150 (MQ=34)

                                                                    gCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCg                                                                                >  1:1188997/1‑150 (MQ=34)

                                                                    gCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCg                                                                                >  1:1188918/1‑150 (MQ=34)

                                                                      ccAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCTCGTCAGGTAGGCGTTCCGTACATCGTg                                                                              <  2:574357/150‑1 (MQ=21)

                                                                      ccAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTg                                                                              <  2:4643846/150‑1 (MQ=34)

                                                                        aGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGt                                                                            <  2:3990747/150‑1 (MQ=34)

                                                                             aCGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTcc                                                                       <  1:2268983/150‑1 (MQ=34)

                                                                               gCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTg                                                                     >  2:3960075/1‑150 (MQ=34)

                                                                                   aCTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACa                                                                 <  2:5198048/150‑1 (MQ=34)

                                                                                    cTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACaa                                                                <  2:5067016/150‑1 (MQ=34)

                                                                                      aCGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAgg                                                              <  2:5101161/150‑1 (MQ=34)

                                                                                       cGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGc                                                             >  1:3380190/1‑150 (MQ=34)

                                                                                          gaagaaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGa                                                          >  2:2084506/1‑150 (MQ=37)

                                                                                              aaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTg                                                      >  2:146997/1‑150 (MQ=37)

                                                                                                cATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGt                                                    >  1:786427/1‑150 (MQ=37)

                                                                                                 aTGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTa                                                   <  2:3050406/150‑1 (MQ=37)

                                                                                                    aTCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgac                                                <  1:2398082/150‑1 (MQ=37)

                                                                                                     tCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgacg                                               >  1:4907852/1‑150 (MQ=37)

                                                                                                          ggTGCTGCCCAGATGGACGGCGCGAACCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGACTTCCTGAACAAGTCCGACCTGGTAGACGACGCt                                          >  2:2539921/1‑150 (MQ=255)

                                                                                                          ggTGCTGCACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCt                                          >  1:3063394/1‑150 (MQ=2)

                                                                                                            tgctgcCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGa                                        <  1:3279571/150‑1 (MQ=37)

                                                                                                             gctgcCCAGCTGGACGGCGCGATCCTGGTTTGATCGGCCGACGATGTTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCTTCAGGTTGGCGTTCCGTACATCGTGGTCTTCCTGAACTAGGCCGACCTTGTAGACGACGCTGAg                                       <  1:2539921/150‑1 (MQ=11)

                                                                                                             gctgcCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAg                                       >  1:2685745/1‑150 (MQ=37)

                                                                                                                 cccAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgc                                   <  1:3924103/150‑1 (MQ=37)

                                                                                                                 cccAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgc                                   <  1:995926/150‑1 (MQ=37)

                                                                                                                 cacAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttc                                   >  2:480945/3‑148 (MQ=2)

                                                                                                                  ccAGATGGACGGCGCTATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgct                                  >  1:3158865/1‑150 (MQ=37)

                                                                                                                  ccAGATGGACGGCGCTATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgct                                  >  1:430204/1‑150 (MQ=37)

                                                                                                                  ccAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgct                                  >  2:3459174/1‑150 (MQ=37)

                                                                                                                   cAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttctc                                 <  1:2741159/150‑2 (MQ=2)

                                                                                                                    aGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTgg                                <  1:2561012/150‑1 (MQ=37)

                                                                                                                      aTGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGaa                              <  2:1133813/150‑1 (MQ=38)

                                                                                                                       tGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAAc                             <  1:2766772/150‑1 (MQ=38)

                                                                                                                       tGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGagc                             <  2:4109412/150‑3 (MQ=255)

                                                                                                                           cGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGt                         >  2:3686878/1‑150 (MQ=39)

                                                                                                                           cGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGt                         <  1:5284870/150‑1 (MQ=255)

                                                                                                                           cGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGt                         <  1:2930077/150‑1 (MQ=255)

                                                                                                                            ggCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGACCTggcc                        <  2:2360943/150‑3 (MQ=37)

                                                                                                                            ggCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTc                        <  1:163001/150‑1 (MQ=39)

                                                                                                                               gcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgag                     <  2:2298385/150‑1 (MQ=39)

                                                                                                                               gcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgag                     >  2:4004716/1‑150 (MQ=39)

                                                                                                                                cgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgaga                    <  2:5103261/150‑1 (MQ=39)

                                                                                                                                 gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                   <  2:1052779/150‑1 (MQ=39)

                                                                                                                                 gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                   <  2:2230726/150‑1 (MQ=39)

                                                                                                                                     cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa               <  1:4004716/150‑1 (MQ=39)

                                                                                                                                     cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa               <  2:354795/150‑1 (MQ=39)

                                                                                                                                        gTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGtt            <  1:3229547/150‑1 (MQ=39)

                                                                                                                                         tttGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTTGTCGAGATGGAAGTTc           >  2:2737200/1‑150 (MQ=255)

                                                                                                                                         tttGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTc           <  1:1767351/150‑1 (MQ=39)

                                                                                                                                           gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTcgc         <  2:1239721/147‑1 (MQ=255)

                                                                                                                                           gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTcgc         <  1:1604452/147‑1 (MQ=255)

                                                                                                                                            gCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgcg        <  2:3253137/150‑1 (MQ=39)

                                                                                                                                             cTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCg                                                                                       >  1:5212988/1‑70 (MQ=255)

                                                                                                                                             cTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGa       <  2:2085160/150‑1 (MQ=39)

                                                                                                                                              tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc      >  1:4986312/1‑150 (MQ=39)

                                                                                                                                               cGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAcc     >  2:2143582/1‑150 (MQ=39)

                                                                                                                                                ggCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTgacgatg                                            <  2:2965964/110‑3 (MQ=255)

                                                                                                                                                  ccgccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgc  >  1:2142348/1‑150 (MQ=39)

CGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGC  >  NC_002947/549505‑549800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`