BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	549,769	G→C	11.3%	L147L (CTG→CTC)	tufB →	elongation factor Tu‑B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	549,769	0	G	C	11.3%	539.0 / 48.9	177	L147L (CTG→CTC)	tufB	elongation factor Tu‑B
Reads supporting (aligned to +/- strand): ref base G (72/85); new base C (5/15); total (77/100)
Fisher's exact test for biased strand distribution p-value = 9.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 193 total aligned reads displayed.

AGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGA  >  NC_002947/549621‑549918

aGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTgg                                                                                                                                                      <  1:2561002/150‑1 (MQ=37)

  aTGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGaa                                                                                                                                                    <  2:1133813/150‑1 (MQ=38)

   tGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACACGGCCGACCTGGTAGACGACGCTGAGCTGCTGGacg                                                                                                                                                   >  1:2828477/1‑148 (MQ=37)

   tGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAAc                                                                                                                                                   <  1:2766772/150‑1 (MQ=38)

       cGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGt                                                                                                                                               >  1:5249573/1‑150 (MQ=39)

       cGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGt                                                                                                                                               >  2:3686878/1‑150 (MQ=39)

       cGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGt                                                                                                                                               <  1:2930077/150‑1 (MQ=255)

       cGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGt                                                                                                                                               <  1:5284870/150‑1 (MQ=255)

        ggCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGCCGCTGAGCTGCTGGAACTGGTc                                                                                                                                              <  1:2335151/150‑1 (MQ=255)

        ggCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGACCTggcc                                                                                                                                              <  2:2360943/150‑3 (MQ=37)

        ggCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTc                                                                                                                                              <  1:163001/150‑1 (MQ=39)

            cgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgaga                                                                                                                                          <  2:3980692/150‑1 (MQ=39)

             gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                                                                                                                                         <  2:2230723/150‑1 (MQ=39)

             gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                                                                                                                                         <  2:1052779/150‑1 (MQ=39)

               tCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAgaactcg                                                                                                                                       <  1:5369862/150‑6 (MQ=255)

                 cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                                     <  1:4004716/150‑1 (MQ=39)

                 cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                                     <  2:354795/150‑1 (MQ=39)

                    gTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGtt                                                                                                                                  <  1:3229547/150‑1 (MQ=39)

                        gCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgcg                                                                                                                              <  2:3253137/150‑1 (MQ=39)

                           cGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAcc                                                                                                                           >  1:1626241/1‑150 (MQ=39)

                           cGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAcc                                                                                                                           >  2:2143582/1‑150 (MQ=39)

                            ggCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACct                                                                                                                          >  1:1934870/1‑150 (MQ=39)

                            ggCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACct                                                                                                                          >  1:1666801/1‑150 (MQ=39)

                                   gATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTcc                                                                                                                   <  1:2452379/150‑1 (MQ=39)

                                    aTGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCAGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCa                                                                                                                  <  1:591708/150‑1 (MQ=255)

                                     tGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCAc                                                                                                                 <  2:385246/150‑1 (MQ=39)

                                      ggcccGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTcgaag                                                                                                                >  2:5028980/4‑146 (MQ=255)

                                          cGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACg                                                                                                            >  1:914205/1‑150 (MQ=39)

                                           gATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGa                                                                                                           <  2:2301326/150‑1 (MQ=39)

                                            aTGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGAc                                                                                                          <  1:4097632/150‑1 (MQ=39)

                                            aTGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGAc                                                                                                          <  2:2850856/150‑1 (MQ=39)

                                                cGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTcc                                                                                                      >  1:1487984/1‑150 (MQ=38)

                                                 gCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTccc                                                                                                     <  2:2006377/150‑1 (MQ=38)

                                                 gCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTccc                                                                                                     >  2:1557656/1‑150 (MQ=38)

                                                 acagaCCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTccc                                                                                                     >  2:2124084/5‑150 (MQ=255)

                                                  cagaCCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCa                                                                                                    <  1:291745/147‑1 (MQ=255)

                                                          tGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGcgacga                                                                                            <  1:3175683/150‑1 (MQ=2)

                                                          tGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGcgacga                                                                                            <  1:4744152/150‑1 (MQ=2)

                                                             gCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgct                                                                                                                       <  1:78147/120‑1 (MQ=39)

                                                             gCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgct                                                                                                                       >  2:78147/1‑120 (MQ=39)

                                                             gCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGacac                                                                                         >  2:4007078/1‑150 (MQ=37)

                                                                caTCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGcgacgaagttcc                                                                                      >  1:1283129/1‑144 (MQ=2)

                                                                  tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                                    >  2:3089818/1‑150 (MQ=37)

                                                                  tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                                    >  2:1035901/1‑150 (MQ=37)

                                                                  tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                                    <  2:4314122/150‑1 (MQ=37)

                                                                         cgctcGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGCGACGAAGTTCCAatcatcaa                                                                             <  2:3112262/146‑2 (MQ=2)

                                                                            ccGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCgg                                                                          >  2:4554927/1‑150 (MQ=34)

                                                                             cGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGt                                                                         >  1:4881103/1‑150 (MQ=34)

                                                                              gTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGtt                                                                        <  2:3514294/150‑1 (MQ=34)

                                                                               tCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTc                                                                       <  2:1487984/150‑1 (MQ=34)

                                                                                   gTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCt                                                                   >  2:518861/1‑150 (MQ=34)

                                                                                         gTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATg                                                             <  1:5053137/150‑1 (MQ=34)

                                                                                          ttCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCATATgg                                                            >  1:2078410/1‑150 (MQ=21)

                                                                                                aCATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTgg                                                      >  2:196830/1‑150 (MQ=37)

                                                                                                aCATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTgg                                                      <  2:3638470/150‑1 (MQ=37)

                                                                                                 cATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGa                                                     <  1:1097917/150‑1 (MQ=37)

                                                                                                  aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                                    <  1:722588/150‑1 (MQ=37)

                                                                                                  aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                                    >  1:652031/1‑150 (MQ=37)

                                                                                                   tCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAg                                                   <  1:210207/150‑1 (MQ=37)

                                                                                                       ggTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCaa                                               <  2:1188997/150‑1 (MQ=37)

                                                                                                         tCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCCTCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAg                                             >  1:4487655/1‑150 (MQ=37)

                                                                                                          cTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAga                                            <  1:2562115/150‑1 (MQ=37)

                                                                                                          cTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAga                                            <  2:3158865/150‑1 (MQ=37)

                                                                                                          cTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAga                                            <  1:3607060/150‑1 (MQ=37)

                                                                                                          cTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAga                                            <  2:2532607/150‑1 (MQ=37)

                                                                                                           ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                                    <  2:1241140/109‑1 (MQ=35)

                                                                                                           ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                                    >  1:1241140/1‑109 (MQ=35)

                                                                                                           ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgac                                           >  2:4398249/1‑150 (MQ=37)

                                                                                                             ccTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacga                                         <  2:2678890/150‑1 (MQ=37)

                                                                                                             ccTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacga                                         >  2:5429590/1‑150 (MQ=37)

                                                                                                              cTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacgac                                        <  2:1337406/150‑1 (MQ=37)

                                                                                                                gatcAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgc                                                                                                                               >  2:4277122/4‑61 (MQ=255)

                                                                                                                gAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgc                                                                                                                               <  1:4277122/61‑1 (MQ=255)

                                                                                                                 aaCAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAAc                                     >  1:4101877/1‑150 (MQ=37)

                                                                                                                   cAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGa                                   >  1:4601671/1‑150 (MQ=37)

                                                                                                                    aaGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTAc                                                                                                             <  2:2509401/75‑1 (MQ=39)

                                                                                                                        ccGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATgg                              <  1:1206205/150‑1 (MQ=35)

                                                                                                                         cGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             <  1:1363742/150‑1 (MQ=35)

                                                                                                                         cGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             >  2:1451477/1‑150 (MQ=35)

                                                                                                                         cGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             >  1:1632586/1‑150 (MQ=35)

                                                                                                                         cGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             >  2:3855933/1‑150 (MQ=35)

                                                                                                                           aCCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAg                                                   <  1:3421201/126‑1 (MQ=35)

                                                                                                                           aCCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAg                                                   >  2:3421201/1‑126 (MQ=35)

                                                                                                                           aCCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAg                                                   <  1:3421160/126‑1 (MQ=35)

                                                                                                                               ggTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTAc                       >  1:218997/1‑150 (MQ=34)

                                                                                                                                     cgacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGt                 <  2:3380190/150‑1 (MQ=34)

                                                                                                                                     cgacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGt                 <  1:2084572/150‑1 (MQ=34)

                                                                                                                                      gacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTc                                                                                       >  2:4633362/1‑79 (MQ=34)

                                                                                                                                           tGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCTGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCaagaa           >  2:1323727/1‑150 (MQ=37)

                                                                                                                                           tGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCaagaa           >  1:1122967/1‑150 (MQ=37)

                                                                                                                                               ctgctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATgg                              >  1:882663/1‑127 (MQ=35)

                                                                                                                                                 gctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGt     >  2:2070401/1‑150 (MQ=37)

                                                                                                                                                 gctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGt     >  2:170624/1‑150 (MQ=37)

                                                                                                                                                  ctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             <  1:2280471/125‑1 (MQ=35)

                                                                                                                                                  ctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATggg                             >  2:2280471/1‑125 (MQ=35)

                                                                                                                                                   tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCaagaa           <  1:4968286/142‑1 (MQ=37)

                                                                                                                                                   tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCaagaa           >  1:1013607/1‑142 (MQ=37)

                                                                                                                                                   tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCaagaa           >  2:4968286/1‑142 (MQ=37)

                                                                                                                                                   tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAg   >  1:3710944/1‑150 (MQ=37)

                                                                                                                                                   tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAg   >  2:794988/1‑150 (MQ=37)

AGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGA  >  NC_002947/549621‑549918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`