BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,549	G→C	5.1%	V162V (GTG→GTC) ‡	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,549	0	G	C	5.1%	661.7 / 18.1	196	V162V (GTG→GTC) ‡	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base G (72/114); new base C (7/3); total (79/117)
Fisher's exact test for biased strand distribution p-value = 9.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 199 total aligned reads displayed.

AAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTT  >  NC_002947/554401‑554698

aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTgt                                                                                                                                                      >  1:3138213/1‑149 (MQ=255)

aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                                                                      >  1:20166/1‑150 (MQ=255)

   agaTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCg                                                                                                                                                   >  1:1029890/1‑150 (MQ=255)

      tCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtc                                                                                                                                                <  2:1441808/150‑1 (MQ=255)

             gCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcgg                                                                                                                                         >  2:4695918/1‑150 (MQ=255)

             gCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcgg                                                                                                                                         <  1:1542227/150‑1 (MQ=255)

              ccGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcggc                                                                                                                                        >  2:3084813/1‑150 (MQ=255)

                ggAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcggcgg                                                                                                                                      <  1:4387029/150‑1 (MQ=255)

                ggAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcggcgg                                                                                                                                      <  2:5087782/150‑1 (MQ=255)

                     tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTg                                                                                                                                    <  2:685304/147‑1 (MQ=255)

                      cGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTga                                                                                                                                <  1:3819795/150‑1 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                               >  1:4036314/1‑150 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                               >  1:2389333/1‑150 (MQ=255)

                          gCTATGCTGGTTGCGCAGAGCTTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAtt                                                                                                                            <  2:1317223/150‑1 (MQ=255)

                          gCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAtt                                                                                                                            <  1:5071406/150‑1 (MQ=255)

                          gCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAtt                                                                                                                            <  1:568766/150‑1 (MQ=255)

                            tATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtc                                                                                                                                                <  2:2637260/128‑1 (MQ=255)

                            tATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGc                                                                                                                          <  2:381305/150‑1 (MQ=255)

                             aTGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCt                                                                                                                         <  1:3461484/150‑1 (MQ=255)

                              tGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTc                                                                                                                        >  2:5174345/1‑150 (MQ=255)

                              tGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTc                                                                                                                        >  2:1248272/1‑150 (MQ=255)

                                cTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGt                                                                                                                      >  2:3245131/1‑150 (MQ=255)

                                 tGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTa                                                                                                                     >  1:5232530/1‑150 (MQ=255)

                                  ggTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAc                                                                                                                    <  2:1947275/150‑1 (MQ=255)

                                    ttGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGGATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCg                                                                                                                  >  2:3477699/1‑150 (MQ=255)

                                     tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                 <  2:3070657/150‑1 (MQ=255)

                                     tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                 <  2:3976223/150‑1 (MQ=255)

                                       cgcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGt                                                                                                               <  2:4600940/150‑1 (MQ=255)

                                       cgcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGt                                                                                                               >  2:3267231/1‑150 (MQ=255)

                                        gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                              <  1:2924444/150‑1 (MQ=255)

                                        gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                              >  2:2959358/1‑150 (MQ=255)

                                        gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                              >  1:4353408/1‑150 (MQ=255)

                                        gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                              <  2:2939333/150‑1 (MQ=255)

                                         cAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTgg                                                                                                             >  1:3790215/1‑150 (MQ=255)

                                          agagCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGt                                                                                                               >  2:2605304/1‑147 (MQ=255)

                                            agCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAt                                                                                                                             >  2:4009631/1‑131 (MQ=255)

                                             gCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATc                                                                                                         <  2:2139942/150‑1 (MQ=255)

                                               gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcg                                                                                                        <  1:1277780/149‑1 (MQ=255)

                                               gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcg                                                                                                        >  2:1277780/1‑149 (MQ=255)

                                                  gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                    <  1:4695918/150‑1 (MQ=255)

                                                   cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                   <  1:3987953/150‑1 (MQ=255)

                                                    tCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAgg                                                                                                  >  2:4482509/1‑150 (MQ=255)

                                                    tCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAgg                                                                                                  >  2:1674624/1‑150 (MQ=255)

                                                      agcagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTc                                                                                                <  2:1140999/150‑1 (MQ=255)

                                                      agcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTc                                                                                                <  2:3945967/150‑1 (MQ=255)

                                                       gcagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgaga                                                                                                                              <  2:2333487/119‑1 (MQ=255)

                                                        cagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                              <  2:168752/150‑1 (MQ=255)

                                                         agcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgtg                                                                                             >  2:799088/1‑150 (MQ=255)

                                                         agcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgtg                                                                                             <  1:272242/150‑1 (MQ=255)

                                                          gcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgtgt                                                                                            <  2:3527741/150‑1 (MQ=255)

                                                            tGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                          <  2:4845035/150‑1 (MQ=255)

                                                            tGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                          <  1:5433823/150‑1 (MQ=255)

                                                            tGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                          <  2:1856399/150‑1 (MQ=255)

                                                                aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCctct                                                                                      <  2:1411591/150‑1 (MQ=255)

                                                                aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCctct                                                                                      <  1:2771545/150‑1 (MQ=255)

                                                                  gCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGc                                                                                    >  2:2465422/1‑150 (MQ=255)

                                                                     gtgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGcaca                                                                                 <  1:1842474/150‑1 (MQ=255)

                                                                         aaTGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCt                                                                             <  2:2050495/150‑1 (MQ=255)

                                                                          gcattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTg                                                                            >  1:4990966/4‑150 (MQ=255)

                                                                          aTGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTg                                                                            >  2:766511/1‑150 (MQ=255)

                                                                             ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAt                                                                                                                             <  2:1629054/98‑1 (MQ=255)

                                                                             ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                         <  2:5445306/150‑1 (MQ=255)

                                                                               ccGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGc                                                                       <  1:3069146/150‑1 (MQ=255)

                                                                                cGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                              <  1:1676682/126‑1 (MQ=255)

                                                                                   cgcgCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGTAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGat                                                                   <  1:2106722/150‑1 (MQ=255)

                                                                                      gcCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATc                                                                <  1:4656889/150‑1 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                              <  1:3426793/150‑1 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                              <  2:5291933/150‑1 (MQ=255)

                                                                                         aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                             <  1:3245131/150‑1 (MQ=255)

                                                                                           gAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                           <  2:4453918/150‑1 (MQ=255)

                                                                                           gAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                           >  2:4121069/1‑150 (MQ=255)

                                                                                              gCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaa                                                        >  1:853277/1‑150 (MQ=255)

                                                                                                gTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaaca                                                      <  1:1198147/150‑1 (MQ=255)

                                                                                                gTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaaca                                                      <  1:2608500/150‑1 (MQ=255)

                                                                                                  gCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTg                                                                                                                                    >  2:2737870/1‑70 (MQ=255)

                                                                                                  gCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTg                                                                                                                                    <  1:2737870/70‑1 (MQ=255)

                                                                                                    ggTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTa                                                  <  2:1775355/150‑1 (MQ=255)

                                                                                                     gTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTAc                                                 <  2:1722018/150‑1 (MQ=255)

                                                                                                       aCAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGa                                               <  2:4931998/150‑1 (MQ=255)

                                                                                                             cGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTca                                         <  1:2630036/150‑1 (MQ=255)

                                                                                                                cATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCacac                                      >  2:2566161/1‑150 (MQ=255)

                                                                                                                 aTGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacc                                     >  2:2845026/1‑150 (MQ=255)

                                                                                                                        ttGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACg                              <  1:3363278/150‑1 (MQ=255)

                                                                                                                        ttGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTATCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGTTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtaaag                              >  1:1639268/1‑146 (MQ=255)

                                                                                                                          ggTGCCAAGGGCATCAAGATCCAGGTGAGTGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGgt                            <  2:845538/150‑1 (MQ=255)

                                                                                                                             gCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGtgtg                         <  1:913011/150‑1 (MQ=255)

                                                                                                                              ccAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGa                        >  1:4282390/1‑150 (MQ=255)

                                                                                                                                 aGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCg                     >  2:5225695/1‑150 (MQ=255)

                                                                                                                                   ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                   <  1:2845026/150‑1 (MQ=255)

                                                                                                                                   ggCATCAAGATCCAGGTGAGCGGTAGTCTCGGCGGTGCTGAGATTGCTCGTACAGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                   <  2:2845920/150‑1 (MQ=255)

                                                                                                                                     cATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgtgt                 <  1:3568125/150‑1 (MQ=255)

                                                                                                                                      aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                >  1:1993083/1‑150 (MQ=255)

                                                                                                                                        cAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGaa              <  1:76107/150‑1 (MQ=255)

                                                                                                                                           gATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGt           >  2:3889353/1‑150 (MQ=255)

                                                                                                                                             tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt         >  2:1663619/1‑150 (MQ=255)

                                                                                                                                               cAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTgg       <  1:2959358/150‑1 (MQ=255)

                                                                                                                                                aGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGa      >  2:431889/1‑150 (MQ=255)

                                                                                                                                                aGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGa      >  2:1074277/1‑150 (MQ=255)

                                                                                                                                                 ggTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGAt     <  1:3084917/150‑1 (MQ=255)

                                                                                                                                                    gAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCtt  <  2:2203641/150‑1 (MQ=255)

AAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTT  >  NC_002947/554401‑554698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`