Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 563,010 T→G 5.4% P30P (CCT→CCG) ‡ rpoA → DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947563,0100TG 5.4% 681.8 / 21.0 203P30P (CCT→CCG) ‡rpoADNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (93/99);  new base G (2/9);  total (95/108)
Fisher's exact test for biased strand distribution p-value = 6.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 215 total aligned reads displayed.

TCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAATTG  >  NC_002947/562861‑563158
                                                                                                                                                     |                                                                                                                                                    
tCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCct                                                                                                                                                      >  1:2511030/1‑150 (MQ=255)
  aaCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                                    >  1:5016676/1‑150 (MQ=255)
  aaCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                                    >  1:1220752/1‑150 (MQ=255)
  aaCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                                    >  2:3626849/1‑150 (MQ=255)
   aCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCtctc                                                                                                                                                   <  2:1581509/150‑1 (MQ=255)
   aCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCtctc                                                                                                                                                   <  2:16245/150‑1 (MQ=255)
     gAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGCCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGa                                                                                                                                                 <  1:2644963/150‑1 (MQ=255)
     gAAAACCTGAGTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGa                                                                                                                                                 <  1:3446110/150‑1 (MQ=255)
       aaaCCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGc                                                                                                                                               <  2:573636/150‑1 (MQ=255)
         aCCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAACCACGCTCGAGCCTCTCGAGCGt                                                                                                                                             <  1:98867/150‑1 (MQ=255)
          ccTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                            <  2:810975/150‑1 (MQ=255)
          ccTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGta                                                                                                                                            <  1:3962674/150‑2 (MQ=255)
            tGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGt                                                                                                                                          >  1:2159361/1‑150 (MQ=255)
            tGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGt                                                                                                                                          >  1:1519541/1‑150 (MQ=255)
               ttGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGtaatttt                                                                                                                                       <  1:1706074/150‑7 (MQ=255)
                tGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaattttg                                                                                                                                      <  1:5194376/150‑8 (MQ=255)
                 ttCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCgg                                                                                                                                     <  2:2523821/149‑1 (MQ=255)
                  tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGc                                                                                                                                    <  1:2274527/150‑1 (MQ=38)
                  tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGc                                                                                                                                    <  1:1812389/150‑1 (MQ=38)
                   cGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                      <  1:2462060/147‑1 (MQ=255)
                   cGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                      >  2:2462060/1‑147 (MQ=255)
                        tctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                            <  2:3482858/136‑1 (MQ=255)
                         ctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAccc                                                                                                                             >  1:4247304/1‑150 (MQ=255)
                          tACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCt                                                                                                                            >  1:3309003/1‑150 (MQ=255)
                           aCTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTg                                                                                                                           <  1:3493536/150‑1 (MQ=255)
                             tCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTggg                                                                                                                         >  1:5043726/1‑150 (MQ=255)
                             tCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTggg                                                                                                                         <  1:4607091/150‑1 (MQ=255)
                               cAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCa                                                                                                                       >  1:5112035/1‑150 (MQ=255)
                                          tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcgc                                                                                                            >  1:108181/1‑150 (MQ=255)
                                          tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcgc                                                                                                            >  1:659301/1‑150 (MQ=255)
                                          tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcgc                                                                                                            >  1:967835/1‑150 (MQ=255)
                                          tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcgc                                                                                                            <  1:3744141/150‑1 (MQ=255)
                                            gAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGcgccg                                                                                                          >  2:4029082/1‑150 (MQ=255)
                                            gAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGcgccg                                                                                                          >  1:4656537/1‑150 (MQ=255)
                                               aaTAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCAt                                                                                                       >  2:2727380/1‑150 (MQ=255)
                                                aTAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATc                                                                                                      <  2:4498300/150‑1 (MQ=255)
                                                     tGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                 <  2:1589681/150‑1 (MQ=255)
                                                     tGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                 <  2:4334984/150‑1 (MQ=255)
                                                      gCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgttg                                                                                                <  1:2599437/150‑1 (MQ=255)
                                                         tCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcc                                                                                             >  1:857436/1‑150 (MQ=255)
                                                         tCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcc                                                                                             <  2:3971629/150‑1 (MQ=255)
                                                           cATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctc                                                                                           <  1:43226/150‑1 (MQ=255)
                                                            aTGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctcc                                                                                          <  1:525464/150‑1 (MQ=255)
                                                            aTGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctcc                                                                                          >  2:5355269/1‑150 (MQ=255)
                                                              gCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCAt                                                                                        >  1:2319965/1‑150 (MQ=255)
                                                                       ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGcgccg                                                                                                          >  2:1297456/1‑123 (MQ=255)
                                                                       ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtg                                                                               >  1:4039493/1‑150 (MQ=255)
                                                                           aaTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                           <  1:2876210/150‑1 (MQ=255)
                                                                              gAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCagta                                                                        <  1:2937075/150‑1 (MQ=255)
                                                                                 ttCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAAc                                                                                                                     >  1:5260057/1‑102 (MQ=255)
                                                                                 ttCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATg                                                                                       >  1:4397838/1‑132 (MQ=255)
                                                                                 ttCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                     <  1:3670059/150‑1 (MQ=255)
                                                                                 gtCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                     <  1:5139139/149‑1 (MQ=255)
                                                                                   ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                                   <  2:2096915/150‑1 (MQ=255)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcg                                                                                                                                 <  1:3879328/82‑4 (MQ=14)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcg                                                                                                                                 <  1:1138288/82‑4 (MQ=14)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcg                                                                                                                                 >  2:1138288/1‑79 (MQ=14)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCga                                                             <  1:5373885/150‑1 (MQ=255)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCga                                                             >  2:3642677/1‑150 (MQ=255)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCga                                                             >  1:4589051/1‑150 (MQ=255)
                                                                                           cccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCgaga                                                           >  1:4832951/1‑150 (MQ=255)
                                                                                            ccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGAt                                                          <  1:4133807/150‑1 (MQ=255)
                                                                                             cGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATc                                                         <  2:742066/150‑1 (MQ=255)
                                                                                                cacaTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGAt                                                      <  2:4123389/150‑1 (MQ=255)
                                                                                                cacaTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGAt                                                      <  1:891115/150‑1 (MQ=255)
                                                                                                 acaTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                                     >  2:135006/1‑150 (MQ=255)
                                                                                                   aTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                   <  2:108181/150‑1 (MQ=255)
                                                                                                   aTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                   <  2:3792584/150‑1 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCg                                                  <  1:127224/150‑1 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCACATCCTGTTGTCCTCCATGCCTGGCTGTGCCGTAGTCGAGGCCGAGATCGATGGCg                                                  >  1:4134569/1‑150 (MQ=255)
                                                                                                      gATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTa                                                <  1:1013240/150‑1 (MQ=255)
                                                                                                         gtgCATGTAGTCAGTCCGACCCGCGACAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGTCCGAGATCTATGGCGTACTc                                             <  2:3276639/150‑1 (MQ=255)
                                                                                                           gCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCa                                           >  1:4504075/1‑150 (MQ=255)
                                                                                                              ggTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                        <  2:4554510/150‑1 (MQ=255)
                                                                                                              ggTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                        <  2:3557799/150‑1 (MQ=255)
                                                                                                                 agtcagtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTa                                     <  2:1648104/150‑1 (MQ=255)
                                                                                                                  gtcagtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTgg                                                                                  >  2:2889415/1‑104 (MQ=255)
                                                                                                                          gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGAt                            >  2:4637422/1‑150 (MQ=255)
                                                                                                                              cgcgCCAACATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                        <  1:4368724/150‑1 (MQ=255)
                                                                                                                              cgcgCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                        <  2:2159361/150‑1 (MQ=255)
                                                                                                                              cgcgCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                        <  2:4961955/150‑1 (MQ=255)
                                                                                                                              cgcgCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                        <  1:736982/150‑1 (MQ=255)
                                                                                                                               gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                       <  2:689210/150‑1 (MQ=255)
                                                                                                                               gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                       <  1:571931/150‑1 (MQ=255)
                                                                                                                                 gcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGgt                     >  1:942296/1‑150 (MQ=255)
                                                                                                                                    aaaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGtt                  >  2:2002348/1‑150 (MQ=255)
                                                                                                                                     aaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTc                 <  2:4391727/150‑1 (MQ=255)
                                                                                                                                      aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCa                <  2:3037199/150‑1 (MQ=255)
                                                                                                                                       aTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAg               <  2:3209524/150‑1 (MQ=255)
                                                                                                                                         caCGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGa             >  2:3941352/2‑150 (MQ=255)
                                                                                                                                           cGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAg           <  1:203179/150‑1 (MQ=255)
                                                                                                                                             cTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc         <  2:1430401/150‑1 (MQ=255)
                                                                                                                                             cTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc         <  1:2214420/150‑1 (MQ=255)
                                                                                                                                              tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCagtag                                                                       >  1:3389515/1‑87 (MQ=255)
                                                                                                                                              tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGacgacg        >  2:4243100/1‑150 (MQ=255)
                                                                                                                                              tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACg        >  2:5280210/1‑150 (MQ=255)
                                                                                                                                               cGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGt       <  1:4865144/150‑1 (MQ=255)
                                                                                                                                                gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGaacgttct      <  2:2327160/150‑7 (MQ=255)
                                                                                                                                                   ccTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAAtt   <  1:1414734/150‑1 (MQ=255)
                                                                                                                                                    ctctCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAATTg  <  1:3206680/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAATTG  >  NC_002947/562861‑563158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑