Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 590,714 T→A 5.6% W279R (TGG→AGG)  PP_0500 → dTDP‑4‑rhamnose reductase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947590,7140TA 5.6% 720.5 / 20.9 214W279R (TGG→AGG) PP_0500dTDP‑4‑rhamnose reductase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (96/105);  new base A (6/6);  total (102/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 218 total aligned reads displayed.

TCGCCGAGGCCGGCCAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTCGACGACTT  >  NC_002947/590565‑590863
                                                                                                                                                      |                                                                                                                                                     
tCGCCGAGGCCGGCCAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCt                                                                                                                                                       >  2:1464912/1‑150 (MQ=255)
tCGCCGAGGCCGGCCAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCt                                                                                                                                                       <  1:2345151/150‑1 (MQ=255)
 cGCCGAGGCCGGCCAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTg                                                                                                                                                      <  1:694122/150‑1 (MQ=255)
         ccggccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTg                                                                                                                                              <  2:2222480/150‑1 (MQ=255)
          cggccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTgg                                                                                                                                             >  1:3861007/1‑150 (MQ=255)
          cggccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTgg                                                                                                                                             <  1:3111168/150‑1 (MQ=255)
          cggccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTgg                                                                                                                                             >  1:437660/1‑150 (MQ=255)
          cggccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTgg                                                                                                                                             >  2:71529/1‑150 (MQ=255)
            gccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCt                                                                                                                                           >  1:5167294/1‑150 (MQ=255)
             ccAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCtt                                                                                                                                          <  2:2925609/150‑1 (MQ=255)
               aaTACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCCAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGc                                                                                                                                        <  1:2906703/150‑1 (MQ=255)
               aaTACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGc                                                                                                                                        >  2:4924174/1‑150 (MQ=255)
               aaTACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGc                                                                                                                                        <  1:3167167/150‑1 (MQ=255)
               aaTACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGc                                                                                                                                        >  1:4003000/1‑150 (MQ=255)
               aaTACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTAGCAAGCCAGCGCGCCAGGCACGC‑GGCTTga                                                                                                                                        <  2:684615/150‑2 (MQ=255)
                 tACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCa                                                                                                                                      <  1:5067151/150‑1 (MQ=255)
                 tACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCa                                                                                                                                      >  1:261391/1‑150 (MQ=255)
                    cGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACct                                                                                                                                   >  1:2046372/1‑150 (MQ=255)
                      ccAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATTCCAGCGAAGAGCCACCGCACGCGGTACTGGCCTGCAAGAAAATCCTGCATACCGCCGGTATCAAGCC‑GCGTGCCTGGCGCGCGGGCTTGCCACctct                                                                                                                                 <  2:3492919/150‑1 (MQ=255)
                        aGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGTCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTAc                                                                                                                               <  2:5103790/150‑1 (MQ=255)
                        aGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTAc                                                                                                                               >  2:263464/1‑150 (MQ=255)
                         gCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTCCt                                                                                                                              <  1:1561734/150‑1 (MQ=255)
                             gCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGAc                                                                                                                          >  2:3434766/1‑150 (MQ=255)
                                gTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTg                                                                                                                                              >  1:1785814/1‑127 (MQ=255)
                                gTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCgg                                                                                                                       >  2:3665783/1‑150 (MQ=255)
                                 ttCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGt                                                                                                                      <  1:1505988/150‑1 (MQ=255)
                                      gCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTAc                                                                                                                 >  1:3312071/1‑150 (MQ=255)
                                       cccccACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTAcc                                                                                                                >  1:4615389/1‑150 (MQ=255)
                                        ccccACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCg                                                                                                               >  1:951/1‑150 (MQ=255)
                                        ccccACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCg                                                                                                               >  2:5427008/1‑150 (MQ=255)
                                          ccACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGcc                                                                                                             >  1:1007167/1‑150 (MQ=255)
                                            aCGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGGACTGGCCTGCAAGAAACTCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCGACCGCCAt                                                                                                           <  2:3100723/150‑1 (MQ=255)
                                             cgccgcAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATg                                                                                                          <  2:486672/150‑1 (MQ=255)
                                                   aGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGAc                                                                                                    <  2:865337/150‑1 (MQ=255)
                                                    ggCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACg                                                                                                   >  2:671147/1‑150 (MQ=255)
                                                    ggCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACg                                                                                                   <  1:35086/150‑1 (MQ=255)
                                                      cccATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGcc                                                                                                 <  2:4327940/150‑1 (MQ=255)
                                                       ccATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGccc                                                                                                <  1:1537449/150‑1 (MQ=255)
                                                       ccATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGccc                                                                                                <  2:1359766/150‑1 (MQ=255)
                                                        cATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGcccc                                                                                               >  1:3573072/1‑150 (MQ=255)
                                                        cATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGcccc                                                                                               <  2:4493089/150‑1 (MQ=255)
                                                        cATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGcccc                                                                                               >  2:824005/1‑150 (MQ=255)
                                                        cATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGcccc                                                                                               <  1:5427008/150‑1 (MQ=255)
                                                            ccGCGCGCCCGGATGCCCGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAACTCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATc                                                                                           >  2:3795402/1‑150 (MQ=255)
                                                                gcgcCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGa                                                                                       <  2:5085027/150‑1 (MQ=255)
                                                                 cgcCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGAt                                                                                      <  1:1185430/150‑1 (MQ=255)
                                                                          gCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACcggcgg                                                                             >  1:1799274/1‑150 (MQ=255)
                                                                            cAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATcc                                                                                          <  2:1276020/135‑1 (MQ=255)
                                                                            cAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTg                                                                           >  1:2787252/1‑150 (MQ=255)
                                                                            cAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTg                                                                           >  1:1741457/1‑150 (MQ=255)
                                                                             aGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCgg                                                                                                                       <  2:1890145/105‑1 (MQ=255)
                                                                             aGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCgg                                                                                                                       >  1:1890145/1‑105 (MQ=255)
                                                                               cGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCg                                                                        <  2:2736346/150‑1 (MQ=255)
                                                                                  agagCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCt                                                                     >  1:2964655/1‑150 (MQ=255)
                                                                                    agCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTc                                                                   <  1:4728116/150‑1 (MQ=255)
                                                                                     gCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCa                                                                  >  1:239571/1‑150 (MQ=255)
                                                                                      ccACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCAt                                                                 >  2:2206640/1‑150 (MQ=255)
                                                                                       cacaGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCAtt                                                                >  2:4218309/1‑150 (MQ=255)
                                                                                         caGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTgg                                                              >  2:5004382/1‑150 (MQ=255)
                                                                                            cACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCtt                                                                    >  1:2792356/1‑141 (MQ=255)
                                                                                            cACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCtt                                                                    <  2:2792356/141‑1 (MQ=255)
                                                                                             aCGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTcc                                                          <  1:4772165/150‑1 (MQ=255)
                                                                                               gcgGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCtt                                                                    <  2:3743704/138‑1 (MQ=255)
                                                                                               gcgGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCtt                                                                    >  1:3743704/1‑138 (MQ=255)
                                                                                                cgGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCAc                                                       <  2:1771677/150‑1 (MQ=255)
                                                                                                cgGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCAc                                                       <  1:437272/150‑1 (MQ=255)
                                                                                                 ggTACTGGCCTGCAAGAAAATCCGTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATGGGCTCCCAcc                                                      <  2:3891811/150‑1 (MQ=255)
                                                                                                     cTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCtgtg                                                  <  1:4060505/150‑1 (MQ=255)
                                                                                                      tGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGc                                                 >  2:4038755/1‑150 (MQ=255)
                                                                                                        gCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGa                                               <  2:1698362/150‑1 (MQ=255)
                                                                                                        gCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGa                                               >  1:2476836/1‑150 (MQ=255)
                                                                                                            gCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCg                                           <  1:3288301/150‑1 (MQ=255)
                                                                                                              aaGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCgtt                                         <  2:2527040/150‑1 (MQ=255)
                                                                                                              aaGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCgtt                                         >  2:3216467/1‑150 (MQ=255)
                                                                                                                 aaaaTCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCgttgtt                                      <  2:3923759/150‑1 (MQ=255)
                                                                                                                     tCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGAc                                  <  1:4924174/150‑1 (MQ=255)
                                                                                                                        ttCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGGCaaa                               <  2:4337389/150‑1 (MQ=255)
                                                                                                                        ttCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACaaa                               >  1:106178/1‑150 (MQ=255)
                                                                                                                         tCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAg                              >  1:5360288/1‑150 (MQ=255)
                                                                                                                         tCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAg                              >  1:370190/1‑150 (MQ=255)
                                                                                                                         tCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAg                              >  1:1283985/1‑150 (MQ=255)
                                                                                                                           aTACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGc                            >  1:2385645/1‑150 (MQ=255)
                                                                                                                            tACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCt                           <  2:4615389/150‑1 (MQ=255)
                                                                                                                             tccTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTa                          <  1:2259690/149‑1 (MQ=255)
                                                                                                                              ccTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTAc                         >  2:1804764/1‑150 (MQ=255)
                                                                                                                              ccTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCAGTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTAc                         >  2:2090720/1‑150 (MQ=255)
                                                                                                                                ttCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGc                       >  2:1113381/1‑150 (MQ=255)
                                                                                                                                   ggtggcAAGCCAGCGCGCCAGGCACGC‑GGCTTGATACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGt                    >  2:4013161/6‑150 (MQ=38)
                                                                                                                                   ggTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGt                    >  2:2300797/1‑150 (MQ=255)
                                                                                                                                         aagccagcgcgcCAGGCACGCGGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTAc                  >  2:2114419/7‑147 (MQ=255)
                                                                                                                                         aagccagcgcgcCAGGCACGCGGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTAc                  <  1:2114419/141‑1 (MQ=255)
                                                                                                                                          aGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCAtt             >  1:1265015/1‑150 (MQ=255)
                                                                                                                                           gCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCgttg                                        >  2:2430361/1‑122 (MQ=255)
                                                                                                                                            cc‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATtct           >  1:1610614/1‑150 (MQ=255)
                                                                                                                                             c‑gcgTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATtctc          <  1:1464912/150‑1 (MQ=255)
                                                                                                                                               gcgTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTcg         <  2:261391/150‑1 (MQ=255)
                                                                                                                                                cgTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTcga        <  2:2377432/150‑1 (MQ=255)
                                                                                                                                                cgTGCCTGGCGCGCTGGCTTGCCACCTCTACGGGACCGGTTCTACCGCCATGGCTGACGCCCCCCTCCTGATCACCGGCAGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTcga        <  2:1446841/150‑1 (MQ=255)
                                                                                                                                                   gCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTcgacga     <  1:220151/150‑1 (MQ=255)
                                                                                                                                                      tGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTCGACGACtt  >  1:1979542/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TCGCCGAGGCCGGCCAATACCGCCAGCTGGCCGTTCAGGCCCCCACGCCGCAGGCCCATGCCGCGCGCCCGGATGCCAGCGAAGAGCCACAGCACGCGGTACTGGCCTGCAAGAAAATCCTTCATACCTTCGGTATCAAGCC‑GCGTGCCTGGCGCGCTGGCTTGCCACCTCTACTGGACCGGTTCTACCGCCATGGCTGACGCCCCCATCCTGATCACCGGCGGTGCCGGCTTCATTGGCTCCCACCTGTGCGATGCGTTGTTGGACAAAGGCTACGCCGTACGCATTCTCGACGACTT  >  NC_002947/590565‑590863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑