BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	855,675	C→G	5.4%	L175V (CTG→GTG) ‡	murI →	glutamate racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	855,675	0	C	G	5.4%	486.0 / 13.6	147	L175V (CTG→GTG) ‡	murI	glutamate racemase
Reads supporting (aligned to +/- strand): ref base C (79/59); new base G (4/4); total (84/63)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 150 total aligned reads displayed.

CCAAGTTCGCAGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCAAT  >  NC_002947/855529‑855824

  aaGTTCGCAGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCt                                                                                                                                                  >  2:4820441/1‑150 (MQ=255)

      tCGCAGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCAGTAGCGGCTGCac                                                                                                                                              <  1:3466299/150‑2 (MQ=255)

          aGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTg                                                                                                                                          >  2:3908916/1‑150 (MQ=255)

          aGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTg                                                                                                                                          >  1:2432497/1‑150 (MQ=255)

          aGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTg                                                                                                                                          <  2:2875054/150‑1 (MQ=255)

             cTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGa                                                                                                                                       <  1:4182998/150‑1 (MQ=255)

             cTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGa                                                                                                                                       <  2:297942/150‑1 (MQ=255)

               tGCTCGATCGCTTCGCAAATGACGTACAGGTTATTCCCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATa                                                                                                                                     <  2:1435908/150‑1 (MQ=255)

                  tCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGc                                                                                                                                  <  2:1885191/150‑1 (MQ=255)

                  tCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGc                                                                                                                                  >  2:313534/1‑150 (MQ=255)

                  tCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGc                                                                                                                                  >  2:1018308/1‑150 (MQ=255)

                     aTCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGa                                                                                                                               >  2:3979455/1‑150 (MQ=255)

                       cGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATc                                                                                                                             <  2:5051007/150‑1 (MQ=255)

                        gCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATcc                                                                                                                            >  2:5345648/1‑150 (MQ=255)

                         cTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCt                                                                                                                           <  2:4049874/150‑1 (MQ=255)

                          ttCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAg                                                                                                                                              >  2:2752502/1‑130 (MQ=255)

                          ttCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTc                                                                                                                          <  2:3797832/150‑1 (MQ=255)

                            cGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCgg                                                                                                                        <  2:2351803/150‑1 (MQ=255)

                              cAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCt                                                                                                                      <  1:681104/150‑1 (MQ=255)

                                 aTGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACACTGATCCTCGGCTGCa                                                                                                                   >  1:2237922/1‑150 (MQ=255)

                                  tGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCAc                                                                                                                  >  1:2142862/1‑150 (MQ=255)

                                      gTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCAt                                                                                                              >  1:4299701/1‑150 (MQ=255)

                                      gTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCAt                                                                                                              >  1:4266667/1‑150 (MQ=255)

                                          aGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTAATGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTAcc                                                                                                          >  2:5219943/1‑150 (MQ=255)

                                           ggTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTAccc                                                                                                         <  1:3725062/150‑1 (MQ=255)

                                            gTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCt                                                                                                        >  1:442836/1‑150 (MQ=255)

                                            gTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCt                                                                                                        >  2:1323075/1‑150 (MQ=255)

                                             ttattaCCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCtt                                                                                                       <  2:2077456/150‑1 (MQ=255)

                                             ttattaCCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCtt                                                                                                       <  1:839950/150‑1 (MQ=255)

                                                  aCCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCtt                                                                                                  >  1:277864/1‑150 (MQ=255)

                                                   cccAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATcc                                                                                                                            >  2:1303344/1‑123 (MQ=255)

                                                   cccAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGACTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTc                                                                                                 >  1:4602936/1‑150 (MQ=255)

                                                    ccAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCAccc                                                                                                                >  2:2388896/1‑134 (MQ=255)

                                                    ccAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCg                                                                                                >  1:10346/1‑150 (MQ=255)

                                                     cAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGc                                                                                               >  2:1217938/1‑150 (MQ=255)

                                                     cAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGc                                                                                               <  1:1765505/150‑1 (MQ=255)

                                                            gCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGc                                                                                        <  1:5027393/150‑1 (MQ=255)

                                                             cccGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCt                                                                                       <  2:1423969/150‑1 (MQ=255)

                                                                ggggCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGc                                                                                    <  1:4134801/150‑1 (MQ=255)

                                                                ggggCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGc                                                                                    <  1:1387002/150‑1 (MQ=255)

                                                                 gggCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGcc                                                                                   >  2:4738015/1‑150 (MQ=255)

                                                                     tGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCa                                                                               <  1:4311394/150‑1 (MQ=255)

                                                                       gTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATg                                                                             >  1:4989791/1‑150 (MQ=255)

                                                                       gTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATg                                                                             >  2:3680471/1‑150 (MQ=255)

                                                                          gAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCAGTAGCGGCTGCac                                                                                                                                              <  1:3863667/82‑2 (MQ=255)

                                                                            gCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTccc                                                                        >  2:4735846/1‑150 (MQ=255)

                                                                               gATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGa                                                                     >  2:744433/1‑150 (MQ=255)

                                                                               gATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGa                                                                     <  1:4735846/150‑1 (MQ=255)

                                                                                aTCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGAt                                                                    <  1:5435080/150‑1 (MQ=255)

                                                                                aTCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGAt                                                                    >  2:3371423/1‑150 (MQ=255)

                                                                                  cGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGa                                                                  >  1:5247454/1‑150 (MQ=255)

                                                                                     aGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGt                                                               >  2:4891314/1‑150 (MQ=255)

                                                                                      gCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTg                                                              <  1:1266917/150‑1 (MQ=255)

                                                                                            gACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGt                                                                           <  1:3272141/131‑1 (MQ=255)

                                                                                             aCCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCcatca                                                       <  1:3212021/150‑1 (MQ=255)

                                                                                               cTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCatcatc                                                     <  2:1231092/150‑1 (MQ=255)

                                                                                               cTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCatcatc                                                     >  1:4513494/1‑150 (MQ=255)

                                                                                                     aGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACAcc                                               >  1:2068603/1‑150 (MQ=255)

                                                                                                      gCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCg                                              >  2:719924/1‑150 (MQ=255)

                                                                                                      gCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCg                                              >  2:379517/1‑150 (MQ=255)

                                                                                                        ccGGCGGTGCACCAGCTGCTGCCAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCAGTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGgc                                            >  1:5271023/1‑150 (MQ=255)

                                                                                                         cggcggTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGgcg                                           >  1:3526668/1‑150 (MQ=255)

                                                                                                         cggcggTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGgcg                                           >  1:3527063/1‑150 (MQ=255)

                                                                                                         cggcggTGCGCCAGCTGCTGCAAGGTTATGAGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACCCCGgcg                                           <  1:5448726/150‑1 (MQ=255)

                                                                                                               tGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCg                                     >  2:4945790/1‑150 (MQ=255)

                                                                                                                 cgcCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGaca                                                 >  1:2564770/1‑136 (MQ=255)

                                                                                                                 cgcCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGaca                                                 <  2:2564770/136‑1 (MQ=255)

                                                                                                                   ccAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGgc                                 >  2:1852043/1‑150 (MQ=255)

                                                                                                                     aGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCTATCATCGACACCGGCGCGGCCGTGgcgc                               >  1:1813911/1‑150 (MQ=255)

                                                                                                                      gctgctGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGcgcg                              >  2:1044481/1‑150 (MQ=255)

                                                                                                                         gctgcACGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGACATCATCGACACCGGCGCGGCCGTGGCGCGTCa                           >  2:5371271/1‑150 (MQ=255)

                                                                                                                           tgcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGt                         >  2:5143110/1‑150 (MQ=255)

                                                                                                                           tgcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGt                         >  2:1974682/1‑150 (MQ=255)

                                                                                                                           tgcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGt                         <  2:643244/150‑1 (MQ=255)

                                                                                                                           tgcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATAAGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACAACGGCGAGGCCGTGGCGCGTCAGt                         >  1:2256263/1‑150 (MQ=255)

                                                                                                                            gcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGtt                        <  2:3046234/150‑1 (MQ=255)

                                                                                                                            gcAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGtt                        <  1:3845644/150‑1 (MQ=255)

                                                                                                                             cAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTg                       >  1:3423766/1‑150 (MQ=255)

                                                                                                                             cAAGGTTATGTGCAGACGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGTCTGCACCCATTACCCTTTCCTTCGCCCAATGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGTCGCGTCAGTTg                       >  1:3706875/1‑150 (MQ=255)

                                                                                                                              aaGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGc                      >  1:1646728/1‑150 (MQ=255)

                                                                                                                              aaGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGc                      >  1:1646559/1‑150 (MQ=255)

                                                                                                                                  ttATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACg                  <  2:4266667/150‑1 (MQ=255)

                                                                                                                                  ttATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACg                  <  2:4299701/150‑1 (MQ=255)

                                                                                                                                   tATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACgg                 <  1:3113513/150‑1 (MQ=255)

                                                                                                                                   tATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACgg                 >  2:3117385/1‑150 (MQ=255)

                                                                                                                                     tgtgCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCt               >  2:662235/1‑150 (MQ=255)

                                                                                                                                      gtgCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATg                                                                             <  1:2330344/87‑1 (MQ=255)

                                                                                                                                      gtgCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATg                                                                             >  2:2330344/1‑87 (MQ=255)

                                                                                                                                      ctgCAGCCAGTAGCGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTg              >  1:1389663/2‑150 (MQ=255)

                                                                                                                                      ctgCAGCCAGTAGCGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTg              >  2:2572458/2‑150 (MQ=255)

                                                                                                                                       tgCAGCCAGTAGCGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTg                                   >  1:751434/1‑128 (MQ=255)

                                                                                                                                       tgCAGCCAGTAGCGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTg                                   <  2:751434/128‑1 (MQ=255)

                                                                                                                                        gCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGtt            <  1:4730887/150‑1 (MQ=255)

                                                                                                                                          aGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTgg          >  2:4685756/1‑150 (MQ=255)

                                                                                                                                              gCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGc      <  2:1646559/150‑1 (MQ=255)

                                                                                                                                              gCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGc      <  2:1646728/150‑1 (MQ=255)

                                                                                                                                                tACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCa    >  2:730695/1‑150 (MQ=255)

                                                                                                                                                  cTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCAAt  <  1:2325137/150‑1 (MQ=255)

                                                                                                                                                  cTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCAAt  <  1:5143110/150‑1 (MQ=255)

                                                                                                                                                  cTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCAAt  >  2:2415008/1‑150 (MQ=255)

CCAAGTTCGCAGCCTTGCTCGATCGCTTCGCAAATGACGTACAGGTTATTACCCAGCCTTGCCCGGGGCTGGTCGAGCTGATCGAAGCCGGTGACCTGGTAAGCCCGGCGGTGCGCCAGCTGCTGCAAGGTTATGTGCAGCCGCTACTGGCTGCAGGTTGCGATACGCTGATCCTCGGCTGCACCCATTACCCTTTCCTTCGCCCATTGCTGGCCGGCATGGTCCCGGATGACGTGGCCATCATCGACACCGGCGCGGCCGTGGCGCGTCAGTTGCAACGGCTGTTGGGTGCCAAT  >  NC_002947/855529‑855824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`