BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	874,543	A→G	7.8%	E40G (GAA→GGA) ‡	PP_0759 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	874,543	0	A	G	7.8%	508.3 / 21.5	167	E40G (GAA→GGA) ‡	PP_0759	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (79/75); new base G (6/7); total (85/82)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 167 total aligned reads displayed.

ACTGGCGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCC  >  NC_002947/874397‑874691

  tGGCGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGcc                                                                                                                                                 <  2:3085484/150‑1 (MQ=255)

     cGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTTCGACGCCAAc                                                                                                                                              <  1:2457300/150‑1 (MQ=255)

      gACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTTCGACGCCAACa                                                                                                                                             <  2:1523547/150‑1 (MQ=255)

      gACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACa                                                                                                                                             <  1:87930/150‑1 (MQ=255)

      gACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTACTGTTGGCGTTCGACGCCAACa                                                                                                                                             <  1:2314691/150‑1 (MQ=255)

         tCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTa                                                                                                                                          <  1:2124629/150‑1 (MQ=255)

         tCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTACTGTTGGCGTTCGACGCCAACAgca                                                                                                                                          <  2:5338260/150‑3 (MQ=255)

            gCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACAt                                                                                                                                       <  1:371863/150‑1 (MQ=255)

            gCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACAt                                                                                                                                       <  2:4633475/150‑1 (MQ=255)

             ccTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATg                                                                                                                                      >  1:3240465/1‑150 (MQ=255)

               tAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGtt                                                                                                                                    <  2:3966424/150‑1 (MQ=255)

               tAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGtt                                                                                                                                    <  2:3966443/150‑1 (MQ=255)

               tAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGtt                                                                                                                                    >  1:1504199/1‑150 (MQ=255)

                    tACGGCGCATGAGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACAAGCGGCGCTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCa                                                                                                                               >  2:4507282/1‑150 (MQ=255)

                         cgcATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGctc                                                                                                                          <  1:991332/150‑1 (MQ=255)

                         cgcATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGctc                                                                                                                          <  1:3372896/150‑1 (MQ=255)

                             tgtgGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGa                                                                                                                      <  1:4373295/150‑1 (MQ=255)

                              gtgGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGAc                                                                                                                     <  1:2901265/150‑1 (MQ=255)

                                  aTAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                 <  1:2766538/150‑1 (MQ=255)

                                  aTAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                 >  2:441849/1‑150 (MQ=255)

                                    aGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGa                                                                                                               <  1:4296546/150‑1 (MQ=255)

                                      ggAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAgg                                                                                                             >  1:1095605/1‑150 (MQ=255)

                                       gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                            >  1:3013015/1‑150 (MQ=255)

                                       gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                            >  1:3310722/1‑150 (MQ=255)

                                       gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                            >  1:2797143/1‑150 (MQ=255)

                                       gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                            >  1:1870604/1‑150 (MQ=255)

                                        aGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTc                                                                                                           >  2:1747358/1‑150 (MQ=255)

                                             tttCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCa                                                                                                      <  2:1665588/150‑1 (MQ=255)

                                                cATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCGGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                   <  1:3222474/150‑1 (MQ=255)

                                                 aTGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATg                                                                                                  >  1:2206626/1‑150 (MQ=255)

                                                      tAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTAcgc                                                                                             <  2:3240465/150‑1 (MQ=255)

                                                      tAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTAcgc                                                                                             >  2:123191/1‑150 (MQ=255)

                                                      tAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTAcgc                                                                                             <  2:3736652/150‑1 (MQ=255)

                                                         gTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCa                                                                                          <  1:2921928/150‑1 (MQ=255)

                                                         gTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCa                                                                                          >  1:4621771/1‑150 (MQ=255)

                                                         gTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCa                                                                                          >  1:3626895/1‑150 (MQ=255)

                                                          tCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGTTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAg                                                                                         >  1:4527232/1‑150 (MQ=255)

                                                           cTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGt                                                                                        >  2:2163431/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGTCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:1239625/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:4283541/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:4283843/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:3419825/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  1:1217523/1‑150 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       <  2:1304396/150‑1 (MQ=255)

                                                            ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:3872982/1‑150 (MQ=255)

                                                                 tcatcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTTCGACGCCAACa                                                                                                                                             <  2:3372673/91‑1 (MQ=255)

                                                                   atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATcag                                                                                <  2:256482/150‑1 (MQ=255)

                                                                          tgctgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGAccc                                                                         >  1:3445171/1‑150 (MQ=255)

                                                                             tgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg                                                                      <  1:1747358/150‑1 (MQ=255)

                                                                             tgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg                                                                      <  1:5220162/150‑1 (MQ=255)

                                                                             tgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg                                                                      <  2:4864022/150‑1 (MQ=255)

                                                                              gAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTc                                                                                       >  2:3688774/1‑132 (MQ=255)

                                                                               aGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGt                                                                    >  2:217310/1‑150 (MQ=255)

                                                                                 tGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGa                                                                  >  1:2918031/1‑150 (MQ=255)

                                                                                  gCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGaa                                                                 <  1:3041570/150‑1 (MQ=255)

                                                                                  gCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGaa                                                                 <  2:5052573/150‑1 (MQ=255)

                                                                                   cTGGCAGCCGGCCAGCAGGATGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGt                                                                                                 >  1:1805948/1‑117 (MQ=255)

                                                                                    tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGc                                                               <  2:4522281/150‑1 (MQ=255)

                                                                                    tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGc                                                               >  1:717750/1‑150 (MQ=255)

                                                                                    tGGCAGCCGGCCAGCAGGATGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCAAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGCTGAAGc                                                               >  1:5218813/1‑150 (MQ=255)

                                                                                        aTCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCg                                                           >  1:709854/1‑150 (MQ=255)

                                                                                        aTCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCg                                                           >  2:1522832/1‑150 (MQ=255)

                                                                                           cgcctgGCAGCCGGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGa                                                          <  2:3622736/145‑1 (MQ=255)

                                                                                               tgGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                    <  2:2918031/150‑1 (MQ=255)

                                                                                               tgGCCGGCGGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGCGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                    <  1:4805278/150‑1 (MQ=255)

                                                                                                 gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGgt                                                  <  2:2322708/150‑1 (MQ=255)

                                                                                                 gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGgt                                                  >  1:1095674/1‑150 (MQ=255)

                                                                                                 gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGgt                                                  <  1:4019465/150‑1 (MQ=255)

                                                                                                       tGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTc                                            <  2:1755470/150‑1 (MQ=255)

                                                                                                       tGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTc                                            >  2:2795683/1‑150 (MQ=255)

                                                                                                        gCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCg                                           >  1:2482035/1‑150 (MQ=255)

                                                                                                         ccAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGa                                          >  2:3131933/1‑150 (MQ=255)

                                                                                                          cAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGAc                                         >  1:4850920/1‑150 (MQ=255)

                                                                                                           aGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAACATCGAGCAAGGGTGTGCTCGACa                                        <  2:551201/150‑1 (MQ=255)

                                                                                                               gctGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                    <  1:3742934/148‑1 (MQ=255)

                                                                                                                     tACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCa                                  >  2:4765777/1‑146 (MQ=255)

                                                                                                                     tACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCa                                  <  1:4765777/146‑1 (MQ=255)

                                                                                                                     tACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGGCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAgcgc                              >  1:2911589/1‑150 (MQ=255)

                                                                                                                      accaccAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGcc                             >  1:1301575/1‑150 (MQ=255)

                                                                                                                        caccaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATc                                                                                  <  2:1865916/95‑1 (MQ=255)

                                                                                                                         accaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                          <  1:2661228/150‑1 (MQ=255)

                                                                                                                         accaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                          <  2:2661467/150‑1 (MQ=255)

                                                                                                                         accaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                          >  1:2961871/1‑150 (MQ=255)

                                                                                                                           caGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGc                        >  1:4390623/1‑150 (MQ=255)

                                                                                                                                gcggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGc                   <  2:1095674/150‑1 (MQ=255)

                                                                                                                                  ggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgt                 <  1:4569295/150‑1 (MQ=255)

                                                                                                                                  ggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgt                 <  1:1388503/150‑1 (MQ=255)

                                                                                                                                    tacTGTTGGCGTTCGACGCCAACGGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTc                                            <  1:798798/119‑1 (MQ=255)

                                                                                                                                    tacTGTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgtgt               >  2:5433152/3‑150 (MQ=255)

                                                                                                                                       tGTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                    >  1:3637751/1‑110 (MQ=255)

                                                                                                                                        gTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAg           >  2:2475095/1‑150 (MQ=255)

                                                                                                                                        gTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAg           <  1:4279509/150‑1 (MQ=255)

                                                                                                                                         ttGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAgg          >  1:5101313/1‑150 (MQ=255)

                                                                                                                                         ttGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAgg          >  1:3572613/1‑150 (MQ=255)

                                                                                                                                            gCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCa       <  1:4283541/150‑1 (MQ=255)

                                                                                                                                              gTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCAtt     <  2:1207857/150‑1 (MQ=255)

                                                                                                                                                 gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTa                                                                                    <  2:473536/68‑1 (MQ=255)

                                                                                                                                                 gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGa                                                                            >  1:4186551/1‑76 (MQ=255)

                                                                                                                                                 gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGa                                                                            <  2:4186551/76‑1 (MQ=255)

                                                                                                                                                 gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGcc  >  2:3626269/1‑150 (MQ=255)

ACTGGCGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCC  >  NC_002947/874397‑874691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`