BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	961,717	C→T	5.2%	G234D (GGC→GAC)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	961,717	0	C	T	5.2%	600.5 / 14.5	193	G234D (GGC→GAC)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand): ref base C (75/106); new base T (6/4); total (81/112)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 198 total aligned reads displayed.

CTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTG  >  NC_002947/961569‑961866

cTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGcc                                                                                                                                                      >  1:5313137/1‑150 (MQ=255)

 ttGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGCCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCg                                                                                                                                                     >  1:1126681/1‑150 (MQ=255)

   gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   <  1:1271720/150‑1 (MQ=255)

   gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   <  2:1104149/150‑1 (MQ=255)

   gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAg                                                                                                                                                   <  2:4674420/150‑1 (MQ=255)

   gtgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCACCTGGTCGAGCTGGCCGaa                                                                                                                                                   <  1:3132008/150‑2 (MQ=255)

    tgCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGc                                                                                                                                                  <  2:1604171/150‑1 (MQ=255)

    ggCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCGGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGc                                                                                                                                                  <  2:3205316/149‑1 (MQ=255)

      cccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAg                                                                                                                                                >  1:1866632/1‑150 (MQ=255)

       ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAgg                                                                                                                                               >  1:2952093/1‑150 (MQ=255)

       ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAgg                                                                                                                                               <  2:3441893/150‑1 (MQ=255)

       ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGGTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAgg                                                                                                                                               >  2:491289/1‑150 (MQ=255)

        cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                              >  2:4295067/1‑150 (MQ=255)

        cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                              <  1:407078/150‑1 (MQ=255)

        cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                              <  1:1294560/150‑1 (MQ=255)

            gCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGTGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                          <  1:3057493/150‑1 (MQ=255)

                tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      <  1:5310355/150‑1 (MQ=255)

                tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      <  1:4840187/150‑1 (MQ=255)

                tCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      <  1:3871454/150‑1 (MQ=255)

                  gTAGTTGCGCAGGTTTCCACCGGGGATGAAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTACTTCAGTTGCGCGGTGAGCCCGCTGCCTACGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                                    >  2:3515198/1‑150 (MQ=255)

                  gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                                    >  2:2674166/1‑150 (MQ=255)

                  gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                                    >  2:2528104/1‑150 (MQ=255)

                  gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                                    >  2:3009891/1‑150 (MQ=255)

                  gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCCGGCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAggcagc                                                                                                                                    <  1:4337730/150‑6 (MQ=37)

                   tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTCATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGACGCCGGTg                                                                                                                                   >  2:3842972/1‑150 (MQ=255)

                   tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTg                                                                                                                                   >  2:2310558/1‑150 (MQ=255)

                     gTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGTCAAGGTAAGGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTACGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGAc                                                                                                                                 >  2:2218672/1‑150 (MQ=255)

                         cgcTGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCg                                                                                                                             <  1:4737504/150‑1 (MQ=255)

                          gcAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCgg                                                                                                                            >  1:509717/1‑150 (MQ=255)

                           cAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGt                                                                                                                           <  1:797786/150‑1 (MQ=255)

                           cAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGt                                                                                                                           >  1:539628/1‑150 (MQ=255)

                               gTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                                       <  1:697941/150‑1 (MQ=255)

                                  ccACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGcc                                                                                                                    <  1:1356260/150‑1 (MQ=255)

                                   caacGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCt                                                                                                                   >  2:3737029/4‑150 (MQ=255)

                                   cACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCt                                                                                                                   <  1:1006219/150‑1 (MQ=255)

                                   cACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCt                                                                                                                   >  2:571669/1‑150 (MQ=255)

                                    aCCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                                  >  1:2348295/1‑150 (MQ=255)

                                     ccGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGa                                                                                                                                  <  2:1128121/133‑1 (MQ=255)

                                         ggATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAccc                                                                                                             <  1:3625655/150‑1 (MQ=255)

                                          gATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGAcccc                                                                                                            <  2:2423141/150‑1 (MQ=255)

                                            tGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGt                                                                                                          >  2:796639/1‑150 (MQ=255)

                                             gCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                                      >  2:4421349/1‑121 (MQ=255)

                                               aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                       <  2:654761/150‑1 (MQ=255)

                                               aGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCa                                                                                                       >  1:2070806/1‑150 (MQ=255)

                                                   ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                                   >  2:2958829/1‑150 (MQ=255)

                                                   ttCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGttt                                                                                                   <  1:196836/150‑1 (MQ=255)

                                                     cGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGc                                                                                                 >  2:2863838/1‑150 (MQ=255)

                                                      ggCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGcc                                                                                                >  1:4212368/1‑150 (MQ=255)

                                                             tAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCgg                                                                                         <  2:1956645/150‑1 (MQ=255)

                                                                  gTCGACAGTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGTCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGTGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTACAGTTTGACGAATCGGCTGcc                                                                                    >  2:4135502/1‑150 (MQ=255)

                                                                     gACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCggg                                                                                 >  1:3959259/1‑150 (MQ=255)

                                                                      acacTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGc                                                                                <  2:2902325/150‑1 (MQ=255)

                                                                       cacTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCg                                                                               >  2:203987/1‑150 (MQ=255)

                                                                        acTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgt                                                                              >  2:1684470/1‑150 (MQ=255)

                                                                        acTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgt                                                                              <  2:4469087/150‑1 (MQ=255)

                                                                        acTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgt                                                                              <  2:1401325/150‑1 (MQ=255)

                                                                         cTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtg                                                                             <  2:3529924/150‑1 (MQ=255)

                                                                            ggcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTg                                                                          >  1:2879396/1‑150 (MQ=255)

                                                                            ggcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTg                                                                          >  1:2382084/1‑150 (MQ=255)

                                                                             gcaggcGTGGCACGGCGGCGTAGTTCGGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                         <  1:1152795/150‑1 (MQ=255)

                                                                             gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGa                                                                         <  1:48909/150‑1 (MQ=255)

                                                                               aggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                       >  2:274430/1‑150 (MQ=255)

                                                                               aggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAgg                                                                       <  1:1795649/150‑1 (MQ=255)

                                                                                   gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGc                                                                   >  1:4520465/1‑150 (MQ=255)

                                                                                   gTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGc                                                                   <  1:2625425/150‑1 (MQ=255)

                                                                                      gCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGcaca                                                                >  2:408565/1‑150 (MQ=255)

                                                                                       cACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACAt                                                               <  1:2218672/150‑1 (MQ=255)

                                                                                        aCGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATc                                                              <  1:4052997/150‑1 (MQ=255)

                                                                                           gcggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAg                                                           <  2:139294/150‑1 (MQ=255)

                                                                                             ggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTc                                                         <  1:1684470/150‑1 (MQ=255)

                                                                                              gcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcg                                                        >  1:3421360/1‑150 (MQ=255)

                                                                                               cgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTcgc                                                       <  1:303232/150‑1 (MQ=255)

                                                                                                 tAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGACGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                                     <  1:2175132/150‑1 (MQ=255)

                                                                                                               ggTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTc                                       <  2:5313137/150‑1 (MQ=255)

                                                                                                                 tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                     <  2:1581626/150‑1 (MQ=255)

                                                                                                                 tGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCAGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTTCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGAACATCCAGTCGCGAGCCAGGCCTTGGTCCt                                     <  1:1914464/150‑1 (MQ=255)

                                                                                                                  gAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTg                                    <  1:1446204/150‑1 (MQ=255)

                                                                                                                   aGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGc                                   <  1:491289/150‑1 (MQ=255)

                                                                                                                   aGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGc                                   <  1:380563/150‑1 (MQ=255)

                                                                                                                    ggCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGca                                  >  1:3651753/1‑150 (MQ=255)

                                                                                                                    ggCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCAGca                                  >  1:3218745/1‑150 (MQ=255)

                                                                                                                      ccGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGcaca                                <  2:4335222/150‑1 (MQ=255)

                                                                                                                             cTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCt                         <  2:3315907/150‑1 (MQ=255)

                                                                                                                             cTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCt                         <  1:2310558/150‑1 (MQ=255)

                                                                                                                              ttCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCtt                        <  1:2690129/150‑1 (MQ=255)

                                                                                                                               tCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTg                       <  1:203987/150‑1 (MQ=255)

                                                                                                                                  gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                    >  1:533419/1‑150 (MQ=255)

                                                                                                                                  gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                    >  1:4223398/1‑150 (MQ=255)

                                                                                                                                  gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcc                    >  1:240584/1‑150 (MQ=255)

                                                                                                                                   cctgCTCGGCCACCTGGTCGAGCTGGCCGAAGGCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCct                   >  1:1657557/4‑150 (MQ=255)

                                                                                                                                   ccAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGcgt                   >  2:3983144/1‑148 (MQ=255)

                                                                                                                                     aGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttc                 <  2:2455165/150‑1 (MQ=255)

                                                                                                                                        tCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGcc                                                  <  2:3128601/114‑1 (MQ=255)

                                                                                                                                        tCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGc                                              >  2:2127256/1‑118 (MQ=255)

                                                                                                                                          gACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTc            <  2:539628/150‑1 (MQ=255)

                                                                                                                                           aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg           <  1:408565/150‑1 (MQ=255)

                                                                                                                                               ggTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAg       <  1:4295067/150‑1 (MQ=255)

                                                                                                                                                 tGGCCGAGCAGGCCGAATCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGTCAttg     <  2:4212655/150‑1 (MQ=255)

                                                                                                                                                 tGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtg     <  2:2841679/150‑1 (MQ=255)

                                                                                                                                                    ccGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAgtggtg  <  2:1492589/150‑1 (MQ=255)

CTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCGGCAGTGGTG  >  NC_002947/961569‑961866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`