BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,148,802	A→C	5.4%	L150R (CTT→CGT)	PP_1007 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,148,802	0	A	C	5.4%	535.7 / 9.4	182	L150R (CTT→CGT)	PP_1007	transmembrane sensor
Reads supporting (aligned to +/- strand): ref base A (90/74); new base C (2/10); total (92/90)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCTTG  >  NC_002947/1148654‑1148951

cTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTTGTGTTGAGCATCACTTTTGCGCCGTCTTCCATTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAg                                                                                                                                                      <  2:2524915/150‑1 (MQ=255)

 tCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAgg                                                                                                                                                     <  1:1143552/150‑1 (MQ=255)

   ggggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtg                                                                                                                                                   >  1:673739/1‑150 (MQ=255)

    gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtgg                                                                                                                                                  <  1:1141288/150‑1 (MQ=255)

      gACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTc                                                                                                                                                <  1:311031/150‑1 (MQ=255)

       aCCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCg                                                                                                                                               >  1:3465737/1‑150 (MQ=255)

         cTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTCGGc                                                                                                                                             <  2:4533993/150‑1 (MQ=255)

            gAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCAGCCTg                                                                                                                                          >  2:3628332/1‑150 (MQ=255)

             aaGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTCGGcgagc                                                                                                                                         <  1:2841753/150‑5 (MQ=255)

               gTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAg                                                                                                                                       <  2:586925/150‑1 (MQ=255)

                tAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGc                                                                                                                                         >  1:2598292/1‑147 (MQ=255)

                tAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGc                                                                                                                                         <  2:2598292/147‑1 (MQ=255)

                tAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAgg                                                                                                                                      >  1:4383413/1‑150 (MQ=255)

                    ccTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCa                                                                                                                                  >  1:2973637/1‑150 (MQ=255)

                    ccTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAAGTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTCAGGTGGTCGGCCTGCAGGCGCa                                                                                                                                  >  2:5332603/1‑150 (MQ=255)

                     cTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCAt                                                                                                                                 >  1:3828772/1‑150 (MQ=255)

                         cccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGc                                                                                                                             <  1:4259176/150‑1 (MQ=255)

                         cccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCGGGCGCTCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGc                                                                                                                             <  1:1919250/150‑1 (MQ=255)

                           cTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCCGGCGCTGGCGCTCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                           <  1:2034715/150‑1 (MQ=255)

                           cTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                           <  1:2815124/150‑1 (MQ=255)

                              tAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCCCCGGAAGGTGGTCGGCCTGCAGGCGCCTGGGCAGGtt                                                                                                                        >  1:3644102/1‑150 (MQ=255)

                                gCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTAc                                                                                                                      >  1:1369862/1‑150 (MQ=255)

                                gCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTAc                                                                                                                      >  1:1769375/1‑150 (MQ=255)

                                       ggcgACCTGGCGGCCGTCCTGGGGTTCGCTGGCGATTGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCTGTTGCAGGCGCGGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGt                                                                                                               <  1:2302474/150‑1 (MQ=255)

                                       ggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGt                                                                                                               <  2:4228290/150‑1 (MQ=255)

                                          gACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGaa                                                                                                            <  2:4423025/150‑1 (MQ=255)

                                           aCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAAt                                                                                                           <  1:2635177/150‑1 (MQ=255)

                                            ccTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATa                                                                                                          <  2:4969026/150‑1 (MQ=255)

                                             cTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAg                                                                                                         <  2:1586081/150‑1 (MQ=255)

                                             cTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTACCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTAACCGACGGTGAATAg                                                                                                         >  2:1195133/1‑150 (MQ=255)

                                              tGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGc                                                                                                        >  2:3985111/1‑150 (MQ=255)

                                                gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGccc                                                                                                      >  1:863415/1‑150 (MQ=255)

                                                gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGccc                                                                                                      <  2:5118542/150‑1 (MQ=255)

                                                       tCCGGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCCGGCGCTGGCGCGCGCCGACCACCGTATGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagc                                                                                               <  1:2539602/150‑1 (MQ=255)

                                                         cTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagcag                                                                                             <  2:1324149/150‑1 (MQ=255)

                                                           ggCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTATGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagcagca                                                                                           <  1:659520/150‑1 (MQ=255)

                                                           ggCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagcagca                                                                                           >  1:1654632/1‑150 (MQ=255)

                                                             cGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCagcagcagc                                                                                         >  2:3347875/1‑150 (MQ=255)

                                                              gTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAgcg                                                                                        >  2:4468726/1‑150 (MQ=255)

                                                                tCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCt                                                                                      <  1:5183639/150‑1 (MQ=255)

                                                                 cGCTGGCGAATGCCGAATTGGTGTTGAGCGGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTg                                                                                     <  2:1423855/150‑1 (MQ=255)

                                                                    tGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGtt                                                                                                                        >  1:2687674/1‑112 (MQ=255)

                                                                    tGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGtt                                                                                                                        <  2:2687674/112‑1 (MQ=255)

                                                                       ctaaTGCCGAATTGGTGTTGACCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTACGGTgg                                                                               <  1:555895/148‑1 (MQ=255)

                                                                             gcGAATTGGTGTTGAGCAGCACTTTTGCGCGGTCTTCCTGTTGCAGGCGCTGGCGCTCGCCGACCTCCGTTTGGTGTTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGAGATGCGGTGGCAAgtg                                                                         <  2:2743165/149‑3 (MQ=255)

                                                                             ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCTCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                         <  1:3080967/150‑1 (MQ=255)

                                                                             ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCAAGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                         >  2:2346431/1‑150 (MQ=255)

                                                                              cGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAgggg                                                                        >  2:3084627/1‑150 (MQ=255)

                                                                              cGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAgggg                                                                        >  1:1339781/1‑150 (MQ=255)

                                                                                  ttGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTTAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTc                                                                    <  2:5009171/150‑1 (MQ=255)

                                                                                    ggTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCa                                                                  <  1:4451456/150‑1 (MQ=255)

                                                                                     gtgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAg                                                                 <  1:1725570/150‑1 (MQ=255)

                                                                                     gtgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAg                                                                 <  1:1667234/150‑1 (MQ=255)

                                                                                      tgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGt                                                                >  1:1799842/1‑150 (MQ=255)

                                                                                       gtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTg                                                               >  2:3140159/1‑150 (MQ=255)

                                                                                       gtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTg                                                               >  1:3762347/1‑150 (MQ=255)

                                                                                       gtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTg                                                               >  2:5207427/1‑150 (MQ=255)

                                                                                        ttGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGTATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGa                                                              >  2:1928438/1‑150 (MQ=255)

                                                                                        gtGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGa                                                              >  1:782816/2‑150 (MQ=255)

                                                                                          gAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACt                                                            >  1:2535043/1‑150 (MQ=255)

                                                                                           agcagcACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTg                                                           >  2:2083471/1‑150 (MQ=255)

                                                                                             cagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcg                                                         >  1:5148885/1‑150 (MQ=255)

                                                                                              agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATgg                                                                                                                               <  2:682456/79‑1 (MQ=255)

                                                                                              agcaCTTTGGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcgc                                                        <  2:1272507/150‑1 (MQ=255)

                                                                                               gcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCa                                                       >  1:4570441/1‑150 (MQ=255)

                                                                                                caCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAg                                                      <  1:1646560/150‑1 (MQ=255)

                                                                                                caCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAg                                                      <  1:736010/150‑1 (MQ=255)

                                                                                                caCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAg                                                      <  1:1281698/150‑1 (MQ=255)

                                                                                                  cTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgc                                                    <  1:1493734/150‑1 (MQ=255)

                                                                                                   ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                                                   >  1:3260940/1‑150 (MQ=255)

                                                                                                   ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                                                   >  2:3558985/1‑150 (MQ=255)

                                                                                                    tttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcgc                                                  <  1:3374298/150‑1 (MQ=255)

                                                                                                       gcgcCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcg                                               >  1:3831913/1‑150 (MQ=255)

                                                                                                        cgcCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcgc                                              >  1:3792445/1‑150 (MQ=255)

                                                                                                         gcCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcgcg                                             >  2:1093792/1‑150 (MQ=255)

                                                                                                         gcCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGGGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcgcg                                             >  1:4669867/1‑150 (MQ=255)

                                                                                                            gTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGACAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCa                                          >  1:4398324/1‑150 (MQ=255)

                                                                                                               ttCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCGGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCCTGa                                       >  1:29049/1‑150 (MQ=255)

                                                                                                                  cAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCt                                    <  1:5207427/150‑1 (MQ=255)

                                                                                                                   aGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGAc                                      <  1:3708704/147‑1 (MQ=255)

                                                                                                                    gTTGCCGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGc                                  <  1:1971959/150‑1 (MQ=255)

                                                                                                                    gTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGc                                  <  2:4383413/150‑1 (MQ=255)

                                                                                                                     ttGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCg                                 >  2:1482969/1‑150 (MQ=255)

                                                                                                                       gCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGtt                               >  2:3339374/1‑150 (MQ=255)

                                                                                                                           gcgctggcgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCCGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgctg                           >  2:2144688/1‑150 (MQ=255)

                                                                                                                                  cgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGc                    <  2:4406604/150‑1 (MQ=255)

                                                                                                                                   gctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCa                   >  1:4439492/1‑150 (MQ=255)

                                                                                                                                      ggCCGACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCAtgt                >  1:3957031/2‑150 (MQ=255)

                                                                                                                                        ccGACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGc              >  1:2892417/1‑150 (MQ=255)

                                                                                                                                         cGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCg             >  2:3258557/1‑150 (MQ=255)

                                                                                                                                           accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTg           <  2:3828772/150‑1 (MQ=255)

                                                                                                                                            ccaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTgg          <  2:487373/150‑1 (MQ=255)

                                                                                                                                             caccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCa                                                                  >  1:4704274/1‑93 (MQ=255)

                                                                                                                                             caccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCa                                                                  <  2:4704274/93‑1 (MQ=255)

                                                                                                                                              accGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGcc        <  1:5332603/150‑1 (MQ=255)

                                                                                                                                               ccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCa       >  1:1603639/1‑150 (MQ=255)

                                                                                                                                               ccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCa       >  1:4724825/1‑150 (MQ=255)

                                                                                                                                                cGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCAc      >  2:2287452/1‑150 (MQ=255)

                                                                                                                                                  tAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCt    >  1:2443990/1‑150 (MQ=255)

                                                                                                                                                    aGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCTtg  <  2:3648879/150‑1 (MQ=255)

CTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCTTG  >  NC_002947/1148654‑1148951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`