BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,244,374	A→G	5.1%	G39G (GGT→GGC) ‡	rnt ←	ribonuclease T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,244,374	0	A	G	5.1%	558.5 / 11.5	176	G39G (GGT→GGC) ‡	rnt	ribonuclease T
Reads supporting (aligned to +/- strand): ref base A (85/81); new base G (6/3); total (91/85)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 188 total aligned reads displayed.

AACTCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTT  >  NC_002947/1244225‑1244523

 aCTCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAaccac                                                                                                                                                      <  2:944451/150‑1 (MQ=255)

   tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCg                                                                                                                                                    <  1:756676/150‑1 (MQ=255)

   tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCg                                                                                                                                                    >  2:798369/1‑150 (MQ=255)

   tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCg                                                                                                                                                    >  2:1117131/1‑150 (MQ=255)

      aGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGtt                                                                                                                                                 >  1:4598210/1‑150 (MQ=255)

      aGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGtt                                                                                                                                                 >  1:3030664/1‑150 (MQ=255)

       gTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGttt                                                                                                                                                >  1:224032/1‑150 (MQ=255)

         gCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCa                                                                                                                                              <  2:2993728/150‑1 (MQ=255)

         gCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCa                                                                                                                                              <  2:2487664/150‑1 (MQ=255)

           ggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAAc                                                                                                                                            <  1:290198/150‑1 (MQ=255)

            gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                           <  1:5165257/150‑1 (MQ=255)

            gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                           <  2:1951587/150‑1 (MQ=255)

            gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACa                                                                                                                                           <  2:3535058/150‑1 (MQ=255)

                 cTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                      <  2:1631602/150‑1 (MQ=255)

                    gATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCcaccac                                                                                                                                   <  2:2171355/150‑1 (MQ=255)

                           gcgcCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTGGGGGAACAGGTAACCTTTCTGTTCACTGCCAATGGTGACGGCGGCAATTTGCAGAAATGCATCGGTAGCGATGTTGAAACCACCTGTTTCAACATCCACCACGACCGGc                                                                                                                            <  1:5150782/150‑1 (MQ=255)

                                 tCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAg                                                                                                                      >  2:1810955/1‑150 (MQ=255)

                                       ggCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAATCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCgg                                                                                                                >  2:4255601/1‑150 (MQ=255)

                                         cTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                              <  1:4107097/150‑1 (MQ=255)

                                         cTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                              >  1:326482/1‑150 (MQ=255)

                                         cTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                              >  2:1265375/1‑150 (MQ=255)

                                         cTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                              <  1:852888/150‑1 (MQ=255)

                                          tCCACTCGGTGGAAGTAGGTATGTTCGGGGAATAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAg                                                                                                             >  1:189928/1‑150 (MQ=255)

                                          tCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTTCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAg                                                                                                             <  2:135263/150‑1 (MQ=255)

                                               tCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTc                                                                                                        >  1:4479954/1‑150 (MQ=255)

                                               tCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCAGGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTc                                                                                                        >  2:5211164/1‑150 (MQ=255)

                                                 ggTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCgg                                                                                                      <  1:1810955/150‑1 (MQ=255)

                                                  gTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGc                                                                                                     <  1:1588974/150‑1 (MQ=255)

                                                      aaGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATc                                                                                                 <  2:5322035/150‑1 (MQ=255)

                                                          aGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGt                                                                                             <  2:4311201/150‑1 (MQ=255)

                                                           ggTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTg                                                                                            >  1:5380967/1‑150 (MQ=255)

                                                           ggTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTg                                                                                            <  1:4785838/150‑1 (MQ=255)

                                                            gTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCg                                                                                                       >  1:5264418/1‑138 (MQ=255)

                                                            gTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCg                                                                                                       <  2:5264418/138‑1 (MQ=255)

                                                                gTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAg                                                                                       <  2:3849822/150‑1 (MQ=255)

                                                                  tCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGa                                                                                     >  2:886610/1‑150 (MQ=255)

                                                                  tCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGa                                                                                     >  1:3410995/1‑150 (MQ=255)

                                                                      ggAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCgc                                                                                 >  2:636130/1‑150 (MQ=255)

                                                                          cAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATGGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCgctgct                                                                             <  1:3310340/150‑1 (MQ=255)

                                                                           aGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCaa                                                                                                                                             <  2:3723106/85‑1 (MQ=255)

                                                                           aGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCaa                                                                                                                                             >  1:3723106/1‑85 (MQ=255)

                                                                            ggAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCtgctgc                                                                           >  1:2593725/1‑150 (MQ=255)

                                                                             gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCcgcg                                                                                                                 >  2:5164290/1‑111 (MQ=255)

                                                                             gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGcc                                                                          >  2:2876727/1‑150 (MQ=255)

                                                                               accctggctcGGCCTTGCCATTGGTGAGGGCGGCAATTTCCAGAATTGCTTGGGTAGCGCTGTTGAAACCACGGGTTTCAACTTCCCCCACGACCGGCTGATCGCCGCGTATGGGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCtt                                                                        <  2:4598210/143‑1 (MQ=255)

                                                                                 ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                      <  1:4366483/150‑1 (MQ=255)

                                                                                 ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                      <  1:3181541/150‑1 (MQ=255)

                                                                                 ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                      <  2:3880429/150‑1 (MQ=255)

                                                                                 ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                      <  1:4276034/150‑1 (MQ=255)

                                                                                   tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCCCCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                    <  2:1849364/150‑1 (MQ=255)

                                                                                   tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                    <  1:4226321/150‑1 (MQ=255)

                                                                                       tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                                <  1:1225374/150‑1 (MQ=255)

                                                                                       tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                                >  2:2271838/1‑150 (MQ=255)

                                                                                       tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                                <  1:4063706/150‑1 (MQ=255)

                                                                                        cGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCa                                                               <  2:4129877/150‑1 (MQ=255)

                                                                                         gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAg                                                              <  2:1282352/150‑1 (MQ=255)

                                                                                         gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAg                                                              >  2:5354396/1‑150 (MQ=255)

                                                                                         gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAg                                                              >  1:4279712/1‑150 (MQ=255)

                                                                                             aTGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTc                                                                                                                                               >  1:4987987/1‑65 (MQ=255)

                                                                                               gCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtcgtc                                                        <  1:3922368/150‑1 (MQ=255)

                                                                                                ccAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCt                                                       >  1:963081/1‑150 (MQ=255)

                                                                                                 cAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCtt                                                      >  1:615703/1‑150 (MQ=255)

                                                                                                  aaTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATCGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTc                                                     <  2:1371013/150‑1 (MQ=255)

                                                                                                  aaTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTc                                                     <  2:2107651/150‑1 (MQ=255)

                                                                                                  aaTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTc                                                     <  2:4063169/150‑1 (MQ=255)

                                                                                                       tGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTaaa                                                >  1:616941/1‑150 (MQ=255)

                                                                                                       tGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTaaa                                                <  2:2579083/150‑1 (MQ=255)

                                                                                                             cggcAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCtt                                          <  1:2049212/150‑1 (MQ=255)

                                                                                                                 agcggccAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCt                                      <  1:1677311/145‑1 (MQ=255)

                                                                                                                  aTTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTc                                     <  1:1241800/150‑1 (MQ=255)

                                                                                                                  aTTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTc                                     >  2:2761661/1‑150 (MQ=255)

                                                                                                                  aTTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTc                                     <  1:636130/150‑1 (MQ=255)

                                                                                                                   tttCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCa                                    >  2:104118/1‑150 (MQ=255)

                                                                                                                      ccAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgc                                 <  2:4516171/150‑1 (MQ=255)

                                                                                                                        aGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGt                               >  2:467865/1‑150 (MQ=255)

                                                                                                                         gCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCCCGCGtt                              >  1:3632326/1‑150 (MQ=255)

                                                                                                                         gCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGtt                              >  2:4981772/1‑150 (MQ=255)

                                                                                                                          cAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCTGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGttt                             <  1:4560737/150‑1 (MQ=255)

                                                                                                                           aaTGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGtttt                            >  1:4610068/1‑150 (MQ=255)

                                                                                                                               cATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtctc                        >  2:2969137/1‑150 (MQ=255)

                                                                                                                               cATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtctc                        <  2:1564183/150‑1 (MQ=255)

                                                                                                                                    gTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCcagca                   >  1:903481/1‑150 (MQ=255)

                                                                                                                                       gcgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgc                <  2:4814383/150‑1 (MQ=255)

                                                                                                                                           tGTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCa            >  2:3175494/1‑150 (MQ=255)

                                                                                                                                           tGTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCa            >  2:3768854/1‑150 (MQ=255)

                                                                                                                                           tGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCa            >  1:4808927/1‑150 (MQ=255)

                                                                                                                                            gTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAg           >  1:1377769/1‑150 (MQ=255)

                                                                                                                                             ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgc          >  2:3813707/1‑150 (MQ=255)

                                                                                                                                               gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcgc        >  2:279352/1‑150 (MQ=255)

                                                                                                                                               gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcgc        >  1:1433916/1‑150 (MQ=255)

                                                                                                                                                aaaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGATGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCa       >  2:3319275/1‑150 (MQ=255)

                                                                                                                                                aaaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCa       >  2:5411308/1‑150 (MQ=255)

                                                                                                                                                aaaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCa       <  1:2162806/150‑1 (MQ=255)

                                                                                                                                                 aaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAg      >  1:3830306/1‑150 (MQ=255)

                                                                                                                                                  accaccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGt     >  1:3238569/1‑150 (MQ=255)

                                                                                                                                                  accaccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGt     >  1:1783661/1‑150 (MQ=255)

                                                                                                                                                   ccaccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtt    <  2:3948567/150‑1 (MQ=255)

                                                                                                                                                    caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGttt   >  1:4799504/1‑150 (MQ=255)

                                                                                                                                                     accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtttt  <  2:3403058/150‑1 (MQ=255)

                                                                                                                                                     accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtttt  >  1:2861137/1‑150 (MQ=255)

AACTCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTT  >  NC_002947/1244225‑1244523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`