Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,256,887 A→T 6.3% noncoding (434/451 nt) PP_mr14 ← cspA regulatory region

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,256,8870AT 6.3% 661.7 / 21.9 202noncoding (434/451 nt)PP_mr14cspA regulatory region
Reads supporting (aligned to +/- strand):  ref base A (96/93);  new base T (6/7);  total (102/100)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

ATTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCT  >  NC_002947/1256739‑1257035
                                                                                                                                                     |                                                                                                                                                    
aTTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAg                                                                                                                                                     >  1:5402814/1‑150 (MQ=255)
aTTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAg                                                                                                                                                     >  1:208123/1‑150 (MQ=255)
  tACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGaa                                                                                                                                                   <  1:1672309/150‑1 (MQ=255)
  tACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGaa                                                                                                                                                   <  1:4564447/150‑1 (MQ=255)
       tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAAACCCGCCAGAAGGCg                                                                                                                                               <  2:1140406/150‑1 (MQ=255)
       tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCgg                                                                                                                                              <  1:710128/150‑1 (MQ=255)
       tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCgg                                                                                                                                              <  1:4014445/150‑1 (MQ=255)
         aGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGt                                                                                                                                            >  1:1535059/1‑150 (MQ=255)
          gCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGtt                                                                                                                                           >  2:2836193/1‑150 (MQ=255)
          gCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGtt                                                                                                                                           >  2:194632/1‑150 (MQ=255)
           cGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttt                                                                                                                                          <  2:1546813/150‑1 (MQ=255)
            gAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGtttt                                                                                                                                         >  2:3661698/1‑150 (MQ=255)
              aGACTGACCTGCCATACCGAGGGTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGtttttt                                                                                                                                       <  2:3253511/150‑1 (MQ=38)
               gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      <  1:81752/150‑1 (MQ=255)
               gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      <  1:81794/150‑1 (MQ=255)
               gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                      <  1:2967059/150‑1 (MQ=255)
                 cTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGtttttttat                                                                                                                                    <  1:4113908/150‑3 (MQ=255)
                 cTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAC‑AAAAAAACCCGCCTTCTGGCGGGtttttttat                                                                                                                                    <  1:2219196/150‑3 (MQ=38)
                  tGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaa                                                                                                                                   >  2:3352224/1‑150 (MQ=255)
                    aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGttt                                                                                                                                          <  1:241934/141‑1 (MQ=255)
                    aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGttt                                                                                                                                          >  2:241934/1‑141 (MQ=255)
                      cTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCt                                                                                                                               >  2:654276/1‑150 (MQ=255)
                      cTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCt                                                                                                                               <  1:3421560/150‑1 (MQ=255)
                       tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                              <  2:2690418/150‑1 (MQ=255)
                       cgCCATCCCGAGTTTGCCCGCCCACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCCGCGCCTATTTCGAGCAGGCAC‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                              <  2:3128574/149‑1 (MQ=255)
                         ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAgg                                                                                                                            <  2:575128/150‑1 (MQ=255)
                           aTACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGtttttttatgcct                                                                                                                                >  1:1974500/1‑138 (MQ=38)
                           aTACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGaa                                                                                                                          >  2:3868425/1‑150 (MQ=255)
                           aTACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGaa                                                                                                                          >  2:3868613/1‑150 (MQ=255)
                           aTACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGaa                                                                                                                          <  2:4322710/150‑1 (MQ=255)
                            tACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAg                                                                                                                         >  1:973718/1‑150 (MQ=255)
                             aCCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAATACCCGCCAGAAGGCGGGTTTTTTTGTAAGCTAGGAAGc                                                                                                                        >  1:1354567/1‑150 (MQ=255)
                             aCCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                        >  1:1696276/1‑150 (MQ=255)
                               cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                      <  1:3797834/150‑1 (MQ=255)
                                gAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAg                                                                                                                     >  1:595842/1‑150 (MQ=255)
                                        ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                             >  1:4796917/1‑150 (MQ=255)
                                        ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                             <  2:3708833/150‑1 (MQ=255)
                                        ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                             >  1:2619686/1‑150 (MQ=255)
                                         cGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTa                                                                                                            >  2:3498002/1‑150 (MQ=255)
                                          gCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                           <  1:4462356/150‑1 (MQ=255)
                                          gCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                           >  2:4462356/1‑150 (MQ=255)
                                              acaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                       >  2:480418/1‑150 (MQ=255)
                                               caGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGAt                                                                                                      <  1:5320887/150‑1 (MQ=255)
                                                   gACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGa                                                                                                  <  2:3363239/150‑1 (MQ=255)
                                                     cTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAAc                                                                                                <  2:396092/150‑1 (MQ=255)
                                                           cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                          >  2:5027469/1‑150 (MQ=255)
                                                            gTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                         <  1:1268701/150‑1 (MQ=255)
                                                                ttGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCt                                                                                     >  2:4030118/1‑150 (MQ=255)
                                                                 tGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTAAGCCTg                                                                                    >  2:2596235/1‑150 (MQ=255)
                                                                  gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATc                                                                                                     >  1:4722054/1‑132 (MQ=255)
                                                                  gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATc                                                                                                     <  2:4722054/132‑1 (MQ=255)
                                                                  gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGc                                                                                   >  2:45392/1‑150 (MQ=255)
                                                                  gAAGCCTGTGCAGTCCCGGGGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGTAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGc                                                                                   >  2:45307/1‑150 (MQ=255)
                                                                        tgtgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCt                                                                             >  2:3138686/1‑150 (MQ=255)
                                                                         gtgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCtt                                                                            <  2:1843198/150‑1 (MQ=255)
                                                                          tgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCttt                                                                           >  2:4671139/1‑150 (MQ=255)
                                                                           gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGa                                                                                                                    <  2:4871885/108‑1 (MQ=255)
                                                                           gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGa                                                                                                                    >  1:4871885/1‑108 (MQ=255)
                                                                           gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                          <  1:2497972/150‑1 (MQ=255)
                                                                           gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                          <  2:3539904/150‑1 (MQ=255)
                                                                             aGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTg                                                                        >  1:1811873/1‑150 (MQ=255)
                                                                              gTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTgg                                                                       >  2:5084989/1‑150 (MQ=255)
                                                                              gTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTgg                                                                       >  2:3455484/1‑150 (MQ=255)
                                                                                 ccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                         <  1:3307121/129‑1 (MQ=255)
                                                                                 ccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcg                                                                    >  2:2263820/1‑150 (MQ=255)
                                                                                  cTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                                   <  1:181845/150‑1 (MQ=255)
                                                                                   tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                                  <  1:194632/150‑1 (MQ=255)
                                                                                    gtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCgg                                                                 <  1:3661698/150‑1 (MQ=255)
                                                                                      gggAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGt                                                               <  1:2703179/150‑1 (MQ=255)
                                                                                       ggAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTa                                                              <  2:5402814/150‑1 (MQ=255)
                                                                                          gCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcg                                                           >  1:497295/1‑150 (MQ=255)
                                                                                             ggCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcgacg                                                        >  1:1438696/1‑150 (MQ=255)
                                                                                              gCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcgacga                                                       <  1:3492657/150‑1 (MQ=255)
                                                                                              gCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcgacga                                                       >  2:1046185/1‑150 (MQ=255)
                                                                                                gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                     <  2:239570/150‑1 (MQ=255)
                                                                                                gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                     <  1:2027899/150‑1 (MQ=255)
                                                                                                  tcccGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGTGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGt                                                   <  1:5402907/149‑1 (MQ=255)
                                                                                                      gcgAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                         <  1:2052542/92‑1 (MQ=255)
                                                                                                        gAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTGTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGc                                             >  2:4154715/1‑150 (MQ=255)
                                                                                                           gagGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGTAGGCGGGATTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTg                                          <  2:4002934/150‑1 (MQ=255)
                                                                                                             ggCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGc                                        <  1:3972304/150‑1 (MQ=255)
                                                                                                                cAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCctt                                     >  1:520878/1‑150 (MQ=255)
                                                                                                                    gcCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCttt                                 >  1:3082156/1‑150 (MQ=255)
                                                                                                                       tATTTCGAGCAGGCAT‑AAAAACACCCGCCAGAAGGCGTGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGATAGTAACAGCCTGGCCTTCTTTCAg                              <  2:3299815/150‑1 (MQ=255)
                                                                                                                       tATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAg                              >  2:2917036/1‑150 (MQ=255)
                                                                                                                        aTTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAgg                             <  1:1046185/150‑1 (MQ=255)
                                                                                                                         tttCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAggg                            >  2:1600672/1‑150 (MQ=255)
                                                                                                                             gAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                        >  1:4927206/1‑150 (MQ=255)
                                                                                                                                cAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGTCTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                     >  2:1350369/1‑150 (MQ=255)
                                                                                                                                cAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                     <  1:4365306/150‑1 (MQ=255)
                                                                                                                                cAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                     <  1:4265569/150‑1 (MQ=255)
                                                                                                                                   gCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                  <  2:1183082/150‑1 (MQ=255)
                                                                                                                                    tttcaaaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTCGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                  >  2:4396613/5‑150 (MQ=255)
                                                                                                                                     aT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                <  2:2074599/150‑1 (MQ=255)
                                                                                                                                        aaaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACGTAGGCGATCTGAACGTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCa              >  2:3021466/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg             >  2:286557/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGc            >  2:2087409/1‑150 (MQ=255)
                                                                                                                                           aaaaCCCGCCTGACGGCGGGTTTTTTTGAACGCGAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCATTCTGGCCGCGGGTAGCGCCGAATGTAACAGCCTGTCCTTCTTTCAGGGTTTTGAAGCCGTCTGCt           <  2:4289026/150‑1 (MQ=255)
                                                                                                                                            aaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGCAAGTAACAGCCTGGCCTTCTGTCAGGGTTTTGAAGCCGTCAGCtt          <  2:5208997/150‑1 (MQ=255)
                                                                                                                                                   ccAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGc   >  2:190499/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCT  >  NC_002947/1256739‑1257035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑