BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,289,857	A→T	5.6%	N375I (AAT→ATT)	PP_1126 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,289,857	0	A	T	5.6%	651.7 / 19.5	198	N375I (AAT→ATT)	PP_1126	hydrolase
Reads supporting (aligned to +/- strand): ref base A (101/86); new base T (6/5); total (107/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 202 total aligned reads displayed.

GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCG  >  NC_002947/1289708‑1290004

gCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCaa                                                                                                                                                      >  1:4828616/1‑150 (MQ=255)

gCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCaa                                                                                                                                                      >  1:5014408/1‑150 (MQ=255)

 cACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAAt                                                                                                                                                     >  1:4773959/1‑150 (MQ=255)

  aCCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATg                                                                                                                                                    >  2:3308095/1‑150 (MQ=255)

     cGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑t                                                                                                                                                >  1:1816802/1‑150 (MQ=255)

      gTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tc                                                                                                                                               >  1:4714677/1‑150 (MQ=255)

         cTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtc                                                                                                                                            <  1:5402181/150‑1 (MQ=255)

          tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcg                                                                                                                                           >  2:409400/1‑150 (MQ=255)

           aTTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGc                                                                                                                                          >  1:429963/1‑150 (MQ=255)

            ttCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCa                                                                                                                                         >  1:4585922/1‑150 (MQ=255)

            ttCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCa                                                                                                                                         >  1:4969973/1‑150 (MQ=255)

            ttCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCa                                                                                                                                         >  1:2232661/1‑150 (MQ=255)

             tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCaa                                                                                                                                        <  1:2090229/150‑1 (MQ=255)

              cccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAg                                                                                                                                       <  1:1673855/150‑1 (MQ=255)

               ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                      <  2:3139824/150‑1 (MQ=255)

               ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                      <  2:1096134/150‑1 (MQ=255)

               ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttgtt                                                                                                                                      <  1:445558/150‑6 (MQ=255)

                cTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAggg                                                                                                                                     >  1:2377507/1‑150 (MQ=255)

                cTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAggg                                                                                                                                     >  1:2377886/1‑150 (MQ=255)

                cTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAggg                                                                                                                                     >  1:1934250/1‑150 (MQ=255)

                 tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCGAGGgc                                                                                                                                    >  1:2820985/1‑150 (MQ=255)

                 tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                    >  2:4366163/1‑150 (MQ=255)

                 tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                    <  1:5295062/150‑1 (MQ=255)

                          aCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCa                                                                                                                           >  2:1495160/1‑150 (MQ=255)

                          aCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCa                                                                                                                           <  2:789191/150‑1 (MQ=255)

                          aCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCa                                                                                                                           >  1:2761912/1‑150 (MQ=255)

                           cGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCaa                                                                                                                          <  1:2509364/150‑1 (MQ=255)

                           cGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCaa                                                                                                                          <  1:2328360/150‑1 (MQ=255)

                            gCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAg                                                                                                                         <  2:1517928/150‑1 (MQ=255)

                             ccGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGc                                                                                                                        >  1:544198/1‑150 (MQ=255)

                                  gcggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAgggg                                                                                                                   >  1:89335/1‑150 (MQ=255)

                                  gcggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAgggg                                                                                                                   >  1:2031785/1‑150 (MQ=255)

                                    ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcg                                                                                                                 >  2:1652421/1‑150 (MQ=255)

                                     gcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgcgc                                                                                                                                  >  1:86666/1‑132 (MQ=255)

                                     gcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcgc                                                                                                                <  1:3629868/150‑1 (MQ=255)

                                     gcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcgc                                                                                                                <  1:3257033/150‑1 (MQ=255)

                                       aGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTg                                                                                                              >  2:3051392/1‑150 (MQ=255)

                                       aGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTg                                                                                                              >  1:2906042/1‑150 (MQ=255)

                                       aGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTg                                                                                                              <  1:467183/150‑1 (MQ=255)

                                       aGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcg                                                                                                                                           <  2:419845/121‑1 (MQ=255)

                                         aCGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGt                                                                                                            >  2:4699135/1‑150 (MQ=255)

                                          cggcggGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGtt                                                                                                           <  2:2203669/150‑1 (MQ=255)

                                           ggcgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTc                                                                                                          >  2:183383/1‑150 (MQ=255)

                                             cgggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGa                                                                                                        <  2:2341471/150‑1 (MQ=255)

                                               ggcgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACt                                                                                                      <  2:259195/150‑1 (MQ=255)

                                                 cgTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTc                                                                                                    <  1:1633004/150‑1 (MQ=255)

                                                   tACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTa                                                                                                  <  2:2143599/150‑1 (MQ=255)

                                                             gCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTgg                                                                                                             >  1:4838826/1‑129 (MQ=255)

                                                              ccGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAcc                                                                                       <  1:3473348/150‑1 (MQ=255)

                                                              ccGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAcc                                                                                       <  1:2167758/150‑1 (MQ=255)

                                                                gggCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTg                                                                                     <  2:4787101/150‑1 (MQ=255)

                                                                  gcgcGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCa                                                                                   <  2:2232798/150‑1 (MQ=255)

                                                                   cgcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAg                                                                                  <  2:3645305/150‑1 (MQ=255)

                                                                    gcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCaa                                                                                                                                <  2:1775226/103‑1 (MQ=255)

                                                                    gcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGTCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGc                                                                                 <  2:4822625/150‑1 (MQ=255)

                                                                            aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                         <  1:2368267/150‑1 (MQ=255)

                                                                            aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                         >  1:261599/1‑150 (MQ=255)

                                                                            aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                         >  1:301167/1‑150 (MQ=255)

                                                                            aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                         >  1:1425295/1‑150 (MQ=255)

                                                                               ttCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGc                                                                      >  2:1886766/1‑150 (MQ=255)

                                                                                tCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCa                                                                     >  1:4204954/1‑150 (MQ=255)

                                                                                 cAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaa                                                                    >  2:4274065/1‑150 (MQ=255)

                                                                                 cAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGACGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaa                                                                    <  2:2954327/150‑1 (MQ=255)

                                                                                     gCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAAccc                                                                <  1:156226/150‑1 (MQ=255)

                                                                                       cTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTg                                                              >  1:2719143/1‑150 (MQ=255)

                                                                                        ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc                                                             >  2:5157468/1‑150 (MQ=255)

                                                                                             cGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGcc                                                        >  1:1734527/1‑150 (MQ=255)

                                                                                              gCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGccc                                                       <  2:379890/150‑1 (MQ=255)

                                                                                                      cGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGa                                               >  2:3000035/1‑150 (MQ=255)

                                                                                                      cGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGa                                               <  1:2493209/150‑1 (MQ=255)

                                                                                                        aTGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAgg                                             <  1:5262213/150‑1 (MQ=255)

                                                                                                          gCACTGGCCGCTTTCTATGGCGAAATCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgc                                           >  1:1859578/1‑150 (MQ=255)

                                                                                                           cACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcc                                          <  1:4869283/150‑1 (MQ=255)

                                                                                                            aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAg                                              >  2:3903450/1‑145 (MQ=255)

                                                                                                            aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAg                                              <  1:3903450/145‑1 (MQ=255)

                                                                                                            aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                         <  2:3881041/150‑1 (MQ=255)

                                                                                                             cTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccag                                        <  1:330484/150‑1 (MQ=255)

                                                                                                              tGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccagc                                       <  2:2173881/150‑1 (MQ=255)

                                                                                                                   gCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCagccagcc                                  >  1:2408722/1‑150 (MQ=255)

                                                                                                                    cTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCg                                 <  2:1425295/150‑1 (MQ=255)

                                                                                                                     tttCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGa                                <  1:3710393/150‑1 (MQ=255)

                                                                                                                     tttCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGa                                <  1:3659078/150‑1 (MQ=255)

                                                                                                                      ttCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAt                               <  2:4828630/150‑1 (MQ=255)

                                                                                                                       tCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAtt                              <  2:4544450/150‑1 (MQ=255)

                                                                                                                         tATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgc                            <  1:4438871/150‑1 (MQ=255)

                                                                                                                           tGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgc                          <  2:429793/150‑1 (MQ=255)

                                                                                                                           tGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgc                          <  2:893588/150‑1 (MQ=255)

                                                                                                                           tGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgc                          <  2:4668293/150‑1 (MQ=255)

                                                                                                                             gCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgcgcgc                        >  1:4230189/1‑150 (MQ=255)

                                                                                                                               gAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGcgccg                      >  2:4935278/1‑150 (MQ=255)

                                                                                                                                aaaGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGcgccgc                     <  2:5299069/150‑1 (MQ=255)

                                                                                                                                     ccttgcgacga‑GGCATTGCCATCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTg                >  2:2891504/6‑150 (MQ=255)

                                                                                                                                       ttgcgacga‑GGCATTGCCATCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGccgccg                   >  1:3833512/4‑145 (MQ=255)

                                                                                                                                       ttgcgacga‑GGCATTGCCATCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCt              >  1:2673128/4‑150 (MQ=255)

                                                                                                                                         acgacgATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTgg            >  2:4599199/1‑150 (MQ=255)

                                                                                                                                             cgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGc        >  1:4880759/1‑150 (MQ=255)

                                                                                                                                              gaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGcc       >  2:741771/1‑150 (MQ=255)

                                                                                                                                                tGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGa     >  1:1136839/1‑150 (MQ=255)

                                                                                                                                                 ggCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGAc    >  2:3734920/1‑150 (MQ=255)

                                                                                                                                                   cAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCg  >  2:5205106/1‑150 (MQ=255)

GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCG  >  NC_002947/1289708‑1290004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`