BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,631	A→C	7.3%	L469R (CTG→CGG) ‡	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,631	0	A	C	7.3%	480.8 / 22.1	150	L469R (CTG→CGG) ‡	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (72/67); new base C (5/6); total (77/73)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

Only 100 of 153 total aligned reads displayed.

ACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1383482‑1383780

  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAgg                                                                                                                                                     <  2:1018931/150‑1 (MQ=255)

  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAgg                                                                                                                                                     >  1:1842112/1‑150 (MQ=255)

     aTGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGcg                                                                                                                                                  >  1:4175709/1‑150 (MQ=255)

        ccTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGcgtcg                                                                                                                                               <  2:1737767/150‑1 (MQ=255)

           gggCTGGTTTTCTTGTCGCGGTCGTCCGGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  2:2771057/150‑1 (MQ=255)

           gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  2:1559243/150‑1 (MQ=255)

           gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:1502492/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  1:5318672/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  2:2947362/150‑1 (MQ=255)

             gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCa                                                                                                                                          <  2:1224908/150‑1 (MQ=255)

              cTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAg                                                                                                                                         <  1:827267/150‑1 (MQ=255)

               tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAga                                                                                                                                        >  2:4227172/1‑150 (MQ=255)

               tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAga                                                                                                                                        <  1:1715723/150‑1 (MQ=255)

               tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACAAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTCCa                                                                                                                                          <  2:4222271/150‑1 (MQ=37)

                    ttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGatgatg                                                                                                                                   <  1:2715087/150‑1 (MQ=255)

                    ttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGatgatg                                                                                                                                   <  2:1484833/150‑1 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  1:2508902/1‑150 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  1:4490727/1‑150 (MQ=255)

                       ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  2:9414/1‑150 (MQ=255)

                          tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  2:4697224/1‑150 (MQ=255)

                          tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  2:1525087/1‑150 (MQ=255)

                          tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  1:4124003/1‑150 (MQ=255)

                            gcgGACGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                           <  2:3389273/150‑1 (MQ=255)

                              ggTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgt                                                                                                                         <  2:3837827/150‑1 (MQ=255)

                               gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                        <  1:2692468/150‑1 (MQ=255)

                               gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                        <  1:1306490/150‑1 (MQ=255)

                                tcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCa                                                                                                                       <  2:5441408/150‑1 (MQ=255)

                                  gtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATa                                                                                                                     <  2:3444021/150‑1 (MQ=255)

                                   tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                    <  1:1018650/150‑1 (MQ=255)

                                   tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                    <  1:1522201/150‑1 (MQ=255)

                                     cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  <  1:373775/150‑1 (MQ=255)

                                     cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  <  1:4569968/150‑1 (MQ=255)

                                     cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  >  1:2039333/1‑150 (MQ=255)

                                     cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  >  1:1638657/1‑150 (MQ=255)

                                      agcagcACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGctc                                                                                                                 >  1:1062933/1‑150 (MQ=255)

                                             cTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGa                                                                                                          <  1:1469114/150‑1 (MQ=255)

                                              ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         <  2:4490727/150‑1 (MQ=255)

                                              ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         >  2:3274276/1‑150 (MQ=255)

                                                cATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATgg                                                                                                       <  1:2301507/150‑1 (MQ=255)

                                                 aTAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGc                                                                                                      >  1:3929203/1‑150 (MQ=255)

                                                  tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTc                                                                                                            <  2:2613758/143‑1 (MQ=255)

                                                  tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTc                                                                                                            >  1:2613758/1‑143 (MQ=255)

                                                  tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                     >  1:3114322/1‑150 (MQ=255)

                                                     ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                  <  1:4697224/150‑1 (MQ=255)

                                                     ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                  >  2:127315/1‑150 (MQ=255)

                                                     ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                  <  2:3903709/150‑1 (MQ=255)

                                                         cggcAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCaa                                                                                              <  2:4284216/150‑1 (MQ=255)

                                                               tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGTCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                        <  1:2864494/150‑1 (MQ=255)

                                                                 agtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                        >  1:3201928/1‑100 (MQ=37)

                                                                 agtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                        <  2:3201928/100‑1 (MQ=37)

                                                                  gtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACggg                                                                                     >  2:1464488/1‑150 (MQ=255)

                                                                   ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                    <  1:4729313/150‑1 (MQ=255)

                                                                     cGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGaca                                                                                  <  1:1548886/150‑1 (MQ=255)

                                                                      ggCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGacac                                                                                 >  2:2297905/1‑150 (MQ=255)

                                                                       gCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACg                                                                                >  2:3953545/1‑150 (MQ=255)

                                                                         aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGcc                                                                              >  1:359266/1‑150 (MQ=255)

                                                                             aGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATg                                                                          <  2:3489641/150‑1 (MQ=255)

                                                                             aGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATg                                                                          >  1:4827671/1‑150 (MQ=255)

                                                                                ttGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:2966342/81‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  2:1063938/150‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  2:1063863/150‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:1542796/150‑1 (MQ=255)

                                                                                  gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:1076557/150‑1 (MQ=255)

                                                                                     ggTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            >  2:3592779/1‑76 (MQ=255)

                                                                                     ggTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:3592779/76‑1 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 >  2:3421668/1‑150 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 >  2:973445/1‑150 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 <  2:1898672/150‑1 (MQ=255)

                                                                                      gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 >  1:85142/1‑150 (MQ=255)

                                                                                       ttGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAg                                                                                                                                         <  2:5085364/77‑1 (MQ=25)

                                                                                       ttGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAg                                                                                                                                         >  1:5085364/1‑77 (MQ=25)

                                                                                         gCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAAccc                                                              <  1:2006530/150‑1 (MQ=255)

                                                                                          cTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCa                                                             <  2:2478587/150‑1 (MQ=255)

                                                                                          cTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCa                                                             >  2:4281205/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:788517/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:3091746/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:4431718/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:5282485/1‑150 (MQ=255)

                                                                                           ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:335783/1‑150 (MQ=255)

                                                                                               cgGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGAc                                                        <  2:4795710/150‑1 (MQ=255)

                                                                                                   ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                    <  2:4998049/150‑1 (MQ=255)

                                                                                                    cACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   >  1:4271612/1‑150 (MQ=255)

                                                                                                            tttCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCt                                           >  1:1009997/1‑150 (MQ=255)

                                                                                                                     gcAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtct                                  >  2:699854/1‑150 (MQ=255)

                                                                                                                        gTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGc                               >  1:651327/1‑150 (MQ=255)

                                                                                                                           ggACCAGGGCA‑‑ATCTGGACCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                            <  1:1827634/150‑1 (MQ=255)

                                                                                                                           ggACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                            <  1:5089824/150‑1 (MQ=255)

                                                                                                                             aCCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGc                          >  2:1906984/1‑150 (MQ=255)

                                                                                                                                aGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                               >  1:3365777/1‑94 (MQ=255)

                                                                                                                                 gggCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATc                                                           >  2:1051750/1‑113 (MQ=255)

                                                                                                                                        aTCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCAc                 >  2:1732310/1‑150 (MQ=255)

                                                                                                                                         tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   <  1:3041694/115‑1 (MQ=38)

                                                                                                                                         tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   >  2:3041694/1‑115 (MQ=38)

                                                                                                                                          cTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACtt               >  2:1998072/1‑150 (MQ=255)

                                                                                                                                            ggAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCa             >  1:377372/1‑150 (MQ=255)

                                                                                                                                               aCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAgcg          >  1:5033493/1‑150 (MQ=255)

                                                                                                                                                    gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     >  1:2043373/1‑86 (MQ=255)

                                                                                                                                                    gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  2:2043373/86‑1 (MQ=255)

                                                                                                                                                       aGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTg  >  1:3659217/1‑150 (MQ=255)

                                                                                                                                                       aGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTg  >  2:413041/1‑150 (MQ=255)

ACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1383482‑1383780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`