BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,632	G→T	7.5%	L469M (CTG→ATG) ‡	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,632	0	G	T	7.5%	477.2 / 20.8	146	L469M (CTG→ATG) ‡	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (71/64); new base T (5/6); total (76/70)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 152 total aligned reads displayed.

CTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1383483‑1383780

 ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAgg                                                                                                                                                     >  1:1842112/1‑150 (MQ=255)

 ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAgg                                                                                                                                                     <  2:1018931/150‑1 (MQ=255)

 ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAgg                                                                                                                                                     >  1:4795710/1‑150 (MQ=255)

          gggCTGGTTTTCTTGTCGCGGTCGTCCGGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  2:2771057/150‑1 (MQ=255)

          gggCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:1502492/150‑1 (MQ=255)

           ggCTGGTTTTCGTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGGGTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTcc                                                                                                                                           <  2:1388472/150‑1 (MQ=255)

            gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  2:2947362/150‑1 (MQ=255)

            gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCa                                                                                                                                          <  1:5318672/150‑1 (MQ=255)

            gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCa                                                                                                                                          <  2:1224908/150‑1 (MQ=255)

             cTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAg                                                                                                                                         <  1:827267/150‑1 (MQ=255)

              tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAga                                                                                                                                        <  1:1715723/150‑1 (MQ=255)

              tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAga                                                                                                                                        >  2:4227172/1‑150 (MQ=255)

              tGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACAAGGGCATCATCTGGAACGACGCCCTGGCTCCGTTCCa                                                                                                                                          <  2:4222271/150‑1 (MQ=37)

                   ttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGatgatg                                                                                                                                   <  1:2715041/150‑1 (MQ=255)

                      ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  2:9414/1‑150 (MQ=255)

                      ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  1:5255654/1‑150 (MQ=255)

                      ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                                >  1:2508902/1‑150 (MQ=255)

                         tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  2:1525087/1‑150 (MQ=255)

                         tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  2:4697224/1‑150 (MQ=255)

                         tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTg                                                                                                                             >  1:4124003/1‑150 (MQ=255)

                           gcgGACGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                           <  2:3389273/150‑1 (MQ=255)

                              gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                        <  1:2692468/150‑1 (MQ=255)

                              gtcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                        <  1:1306490/150‑1 (MQ=255)

                               tcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCa                                                                                                                       <  2:5441408/150‑1 (MQ=255)

                               tcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCa                                                                                                                       <  2:1222114/150‑1 (MQ=255)

                                 gtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATa                                                                                                                     <  2:3444021/150‑1 (MQ=255)

                                  tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                    <  1:1522201/150‑1 (MQ=255)

                                  tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                    <  1:1018650/150‑1 (MQ=255)

                                   ccAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGc                                                                                                                   >  2:1060664/1‑150 (MQ=255)

                                   ccAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGc                                                                                                                   >  2:3564410/1‑150 (MQ=255)

                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  <  1:373775/150‑1 (MQ=255)

                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  >  1:1638657/1‑150 (MQ=255)

                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  <  1:4569968/150‑1 (MQ=255)

                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGct                                                                                                                  >  1:2039333/1‑150 (MQ=255)

                                     agcagcACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGctc                                                                                                                 >  1:1062933/1‑150 (MQ=255)

                                     agcagcACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGctc                                                                                                                 >  1:3903709/1‑150 (MQ=255)

                                            cTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGa                                                                                                          <  1:1469114/150‑1 (MQ=255)

                                             ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         >  1:459827/1‑150 (MQ=255)

                                             ttCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                         <  2:4490727/150‑1 (MQ=255)

                                                 tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTc                                                                                                            <  2:2613758/143‑1 (MQ=255)

                                                 tAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                     >  1:3114322/1‑150 (MQ=255)

                                                    ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                           <  2:1576966/125‑1 (MQ=255)

                                                    ccGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCt                                                                                                  <  2:3903709/150‑1 (MQ=255)

                                                        cggcAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCaa                                                                                              <  2:4284216/150‑1 (MQ=255)

                                                              tcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGTCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCacac                                                                                        <  1:2864494/150‑1 (MQ=255)

                                                                agtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACgg                                                                                      <  2:1954965/150‑1 (MQ=255)

                                                                agtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                        <  2:3201928/100‑1 (MQ=37)

                                                                agtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                        >  1:3201928/1‑100 (MQ=37)

                                                                 gtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACggg                                                                                     >  2:1464488/1‑150 (MQ=255)

                                                                  ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGa                                                                                    <  1:4729313/150‑1 (MQ=255)

                                                                    cGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGaca                                                                                  <  1:1548886/150‑1 (MQ=255)

                                                                     ggCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGacac                                                                                 <  1:4445106/150‑1 (MQ=255)

                                                                     ggCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGacac                                                                                 >  2:2297905/1‑150 (MQ=255)

                                                                        aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGcc                                                                              >  1:359266/1‑150 (MQ=255)

                                                                         tACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCa                                                                             >  1:618993/1‑150 (MQ=255)

                                                                                tGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGt                                                                      >  1:5064523/1‑150 (MQ=255)

                                                                                 gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:1542796/150‑1 (MQ=255)

                                                                                 gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  1:1076557/150‑1 (MQ=255)

                                                                                 gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     <  2:1063863/150‑1 (MQ=255)

                                                                                    ggTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            >  2:3592779/1‑76 (MQ=255)

                                                                                    ggTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTc                                                                                                                                            <  1:3592779/76‑1 (MQ=255)

                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGGCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 >  2:3421710/1‑150 (MQ=255)

                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCa                                                                                                     <  1:956429/114‑1 (MQ=255)

                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 <  2:1898672/150‑1 (MQ=255)

                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCaa                                                                 >  2:973445/1‑150 (MQ=255)

                                                                                      ttGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGACGCCCTGGCTCCGTTCCAg                                                                                                                                         <  2:5085364/77‑1 (MQ=25)

                                                                                        gCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAAccc                                                              <  1:2006530/150‑1 (MQ=255)

                                                                                         cTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCa                                                             <  2:2478587/150‑1 (MQ=255)

                                                                                         cTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCa                                                             >  2:4281205/1‑150 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAg                                                                                                                                                      >  2:2235663/1‑60 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAg                                                                                                                                                      <  1:2235663/60‑1 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:788517/1‑150 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:3091746/1‑150 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  2:4431718/1‑150 (MQ=255)

                                                                                          ttCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCAt                                                            >  1:335783/1‑150 (MQ=255)

                                                                                              cgGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGAc                                                        <  2:4795710/150‑1 (MQ=255)

                                                                                               ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACa                                                       <  2:1842112/150‑1 (MQ=255)

                                                                                                  ccACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTa                                                    <  2:4998049/150‑1 (MQ=255)

                                                                                                   cACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   >  1:4271612/1‑150 (MQ=255)

                                                                                                      gTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGAc                                                <  1:2581728/150‑1 (MQ=255)

                                                                                                           tttCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCt                                           >  1:1009997/1‑150 (MQ=255)

                                                                                                               gTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGcc                                       <  1:2958290/150‑1 (MQ=255)

                                                                                                               gTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGcc                                       <  2:1521109/150‑1 (MQ=255)

                                                                                                                    gcAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTtct                                  >  2:699854/1‑150 (MQ=255)

                                                                                                                          ggACCAGGGCA‑‑ATCTGGACCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                            <  1:1827634/150‑1 (MQ=255)

                                                                                                                          ggACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCa                            <  1:5089824/150‑1 (MQ=255)

                                                                                                                           gACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGGCACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAg                           >  2:3852251/1‑150 (MQ=255)

                                                                                                                               aGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                               >  1:3365777/1‑94 (MQ=255)

                                                                                                                               aGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                               <  2:3365777/94‑1 (MQ=255)

                                                                                                                                       aTCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCAc                 >  2:1732310/1‑150 (MQ=255)

                                                                                                                                        tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATTGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACt                >  1:4169430/1‑150 (MQ=255)

                                                                                                                                        tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   >  2:3041694/1‑115 (MQ=38)

                                                                                                                                        tCTGGAACGACGCCCTGGCTCCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTAc                                                   <  1:3041694/115‑1 (MQ=38)

                                                                                                                                         cTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACtt               >  2:1998072/1‑150 (MQ=255)

                                                                                                                                           ggAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCa             >  1:377372/1‑150 (MQ=255)

                                                                                                                                              aCGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAgcg          >  1:5033493/1‑150 (MQ=255)

                                                                                                                                                   gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                     >  1:2043373/1‑86 (MQ=255)

                                                                                                                                                   gCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCg     >  2:4005671/1‑150 (MQ=255)

                                                                                                                                                      aGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTg  >  2:413041/1‑150 (MQ=255)

                                                                                                                                                      aGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTg  >  1:3659217/1‑150 (MQ=255)

CTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCA‑‑ATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCGCTG  >  NC_002947/1383483‑1383780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`