BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,471,405	A→C	6.4%	S477A (TCG→GCG)	alg8 ←	glycosyltransferase Alg8

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,471,405	0	A	C	6.4%	567.4 / 20.5	173	S477A (TCG→GCG)	alg8	glycosyltransferase Alg8
Reads supporting (aligned to +/- strand): ref base A (77/85); new base C (3/8); total (80/93)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 175 total aligned reads displayed.

CGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGG  >  NC_002947/1471256‑1471553

   tGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGa                                                                                                                                                   <  1:4514458/150‑1 (MQ=255)

       cTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAg                                                                                                                                               >  2:2526612/1‑150 (MQ=255)

       cTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAg                                                                                                                                               <  2:4973977/150‑1 (MQ=255)

         tCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCTCGCGGACCatga                                                                                                                                             <  1:3006286/150‑4 (MQ=255)

         tCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCTCGCGGACCatga                                                                                                                                             <  1:1764455/150‑4 (MQ=255)

          cGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGgtg                                                                                                                                            >  2:2411336/1‑150 (MQ=255)

            gACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGtt                                                                                                                                          <  2:952050/150‑1 (MQ=255)

              cTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGa                                                                                                                                        <  2:424785/150‑1 (MQ=255)

              cTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGa                                                                                                                                        <  1:2426660/150‑1 (MQ=255)

               tCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGaa                                                                                                                                       >  1:4392988/1‑150 (MQ=255)

               tCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGaa                                                                                                                                       >  1:572727/1‑150 (MQ=255)

               tCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCGGTTAGATCCGCTCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGaa                                                                                                                                       >  1:5083047/1‑150 (MQ=255)

                 aTGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAAcc                                                                                                                                     <  1:166253/150‑1 (MQ=255)

                   gCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCAt                                                                                                                                   <  2:5415847/150‑1 (MQ=255)

                   gCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCAt                                                                                                                                   >  2:1849556/1‑150 (MQ=255)

                    cACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCAtt                                                                                                                                  <  1:4645129/150‑1 (MQ=255)

                     aCGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCAttg                                                                                                                                 >  2:2115743/1‑150 (MQ=255)

                       gACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCTCGCGGACCatga                                                                                                                                             >  1:2729581/1‑133 (MQ=255)

                       gACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCTCGCGGACCatga                                                                                                                                             <  2:2729581/136‑4 (MQ=255)

                       gACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCAttgtt                                                                                                                               <  1:2690474/150‑1 (MQ=255)

                          aTTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGaa                                                                                                                            <  1:5385062/150‑1 (MQ=255)

                           ttGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGaaa                                                                                                                           <  1:3712632/150‑1 (MQ=255)

                           ttGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGaaa                                                                                                                           <  1:2200215/150‑1 (MQ=255)

                           ttGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTAGaaa                                                                                                                           <  2:3484370/150‑1 (MQ=255)

                               cGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTg                                                                                                                       >  1:2546827/1‑150 (MQ=255)

                                gTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTgg                                                                                                                      >  2:3784841/1‑150 (MQ=255)

                                    aCGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAg                                                                                                                  <  1:4781021/150‑1 (MQ=255)

                                      ggcggTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGt                                                                                                                <  2:2850020/150‑1 (MQ=255)

                                               cATGGTGGTTTGTTCCTGTTAGATCCGATCCGTGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGa                                                                                                       <  1:1952127/150‑1 (MQ=255)

                                                aTGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAg                                                                                                      <  2:1737532/150‑1 (MQ=255)

                                                aTGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAg                                                                                                      <  1:477801/150‑1 (MQ=255)

                                                  ggtggtTTGTTGGTGTTAGATCCGTTCCGGGTTGGGCTCTCACGACCATGAACAGCACAGCAACGAAGATGCTGGCTGCCGAGAAGGTCATGGTGCGCGAGGACCAGGTGTTGAAGCATTGTTGTATGCTGGCGAGGCCACGCTTGAggg                                                                                                    >  2:1678142/1‑150 (MQ=255)

                                                  ggtggtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAg                                                                                                                                               >  2:2997782/1‑107 (MQ=255)

                                                  ggtggtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAg                                                                                                                                               <  1:2997782/107‑1 (MQ=255)

                                                  ggtggtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAggg                                                                                                    <  2:1581551/150‑1 (MQ=255)

                                                   gtggtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCCTGGTCCTCGCGGACCatga                                                                                                                                             <  2:627197/108‑4 (MQ=255)

                                                     ggtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAg                                                                                                 >  2:92840/1‑150 (MQ=255)

                                                      gtTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGt                                                                                                <  2:964573/150‑1 (MQ=255)

                                                        ttGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTgg                                                                                              <  2:4642981/150‑1 (MQ=255)

                                                           ttCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCt                                                                                           >  2:4604582/1‑150 (MQ=255)

                                                           ttCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCt                                                                                           <  1:2153392/150‑1 (MQ=255)

                                                           ttCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCt                                                                                           >  1:3296579/1‑150 (MQ=255)

                                                             ccTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGTTGa                                                                                         >  2:338063/1‑150 (MQ=255)

                                                               tGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGAcg                                                                                       >  1:4300810/1‑150 (MQ=255)

                                                               tGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGAcg                                                                                       >  1:1945282/1‑150 (MQ=255)

                                                                 ttAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGa                                                                                                       >  1:1891130/1‑132 (MQ=255)

                                                                     atccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGgt                                                                                                                                             >  2:3109936/1‑90 (MQ=255)

                                                                     atccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGgt                                                                                                                                             <  1:3109936/90‑1 (MQ=255)

                                                                     atccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGTGGGCAGTGGGCTGACGCGTCCa                                                                                 >  1:4863106/1‑150 (MQ=255)

                                                                     atccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCa                                                                                 <  1:971270/150‑1 (MQ=255)

                                                                      tccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTggg                                                                                             <  2:4523555/137‑1 (MQ=255)

                                                                       ccgatccgGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACg                                                                               >  1:1139025/1‑150 (MQ=255)

                                                                            ccgGGTTGGGCTCACACGACAATTAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGc                                                                          <  1:3632462/150‑1 (MQ=255)

                                                                              gggTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCtt                                                                        <  2:1945282/150‑1 (MQ=255)

                                                                              gggTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCtt                                                                        <  1:4204598/150‑1 (MQ=255)

                                                                              gggTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCtt                                                                        >  2:4220988/1‑150 (MQ=255)

                                                                                 ttGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTc                                                                     <  2:3730215/150‑1 (MQ=255)

                                                                                    ggCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAg                                                                  <  1:5285410/150‑1 (MQ=255)

                                                                                     gCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAgg                                                                 <  1:4493295/150‑1 (MQ=255)

                                                                                     gCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAgg                                                                 >  1:4020656/1‑150 (MQ=255)

                                                                                      cTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGc                                                                <  1:4300594/150‑1 (MQ=255)

                                                                                      cTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGc                                                                <  1:666063/150‑1 (MQ=255)

                                                                                         acacGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGga                                                             >  2:2409775/1‑150 (MQ=255)

                                                                                          cacGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGgaa                                                            <  1:1758104/150‑1 (MQ=255)

                                                                                             gACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGAc                                                                             >  1:4426399/1‑130 (MQ=255)

                                                                                             gACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGgaagaa                                                         >  1:4931062/1‑150 (MQ=255)

                                                                                             gACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGgaagaa                                                         >  2:2304916/1‑150 (MQ=255)

                                                                                              aCCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAac                                                        <  1:79224/150‑1 (MQ=255)

                                                                                                cATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAacac                                                      <  2:3234926/150‑1 (MQ=255)

                                                                                                  tGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGt                                                    <  1:4639931/150‑1 (MQ=255)

                                                                                                   gAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTa                                                   >  2:1152866/1‑150 (MQ=255)

                                                                                                    aaCAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAg                                                  <  1:1849556/150‑1 (MQ=255)

                                                                                                       agcacagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATc                                               >  2:2827647/1‑150 (MQ=255)

                                                                                                        gcacagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCt                                              <  1:1274513/150‑1 (MQ=255)

                                                                                                        gcacagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCt                                              >  2:752711/1‑150 (MQ=255)

                                                                                                        gcacagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCt                                              >  1:4143132/1‑150 (MQ=255)

                                                                                                         cacagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCtt                                             <  1:832895/150‑1 (MQ=255)

                                                                                                          acagcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTtc                                            >  1:4115119/1‑150 (MQ=255)

                                                                                                            agcaACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTtcat                                          <  2:4115119/150‑1 (MQ=255)

                                                                                                               aaCGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAg                                       <  1:3193724/150‑1 (MQ=255)

                                                                                                                  gAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAgcgc                                    >  2:1629230/1‑150 (MQ=255)

                                                                                                                    aGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAgcgcgc                                  <  1:4091868/150‑1 (MQ=255)

                                                                                                                      aTGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCg                                >  1:5139632/1‑150 (MQ=255)

                                                                                                                           ggCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCgatgat                           >  2:2281050/1‑150 (MQ=255)

                                                                                                                            gCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATc                          <  1:2262908/150‑1 (MQ=255)

                                                                                                                                   agaAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTggtggt                   >  2:4812311/1‑150 (MQ=255)

                                                                                                                                       ggTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATa               >  1:989630/1‑150 (MQ=255)

                                                                                                                                       ggTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATa               <  1:248950/150‑1 (MQ=255)

                                                                                                                                        gTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATag              >  2:1142820/1‑150 (MQ=255)

                                                                                                                                        gTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATag              >  1:3451422/1‑150 (MQ=255)

                                                                                                                                         tCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATaga             >  2:623877/1‑150 (MQ=255)

                                                                                                                                         tCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATaga             <  1:1572727/150‑1 (MQ=255)

                                                                                                                                         tCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTGGTAATaga             >  2:2003067/1‑150 (MQ=255)

                                                                                                                                          cATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATagag            >  1:5063817/1‑150 (MQ=255)

                                                                                                                                             ggTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAAt         >  2:4437957/1‑150 (MQ=255)

                                                                                                                                               tCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTGGTAATAGAGAATCa       >  2:5355569/1‑150 (MQ=255)

                                                                                                                                                ccGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAg      >  1:5344632/1‑150 (MQ=255)

                                                                                                                                                 cgcgAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGc     >  2:3280493/1‑150 (MQ=255)

                                                                                                                                                  gcgAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCg    <  2:4392988/150‑1 (MQ=255)

                                                                                                                                                    gAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCggg  >  1:3371564/1‑150 (MQ=255)

CGCTGGGCTTCGGACTCATGCACGACATTGACGTTCACGGCGGTATTCATGGTGGTTTGTTCCTGTTAGATCCGATCCGGGTTGGGCTCACACGACCATGAACAGCACAGCAACGAAGATGCTGGCAGCCGAGAAGGTCATGGTCCGCGAGGACCAGGTGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGG  >  NC_002947/1471256‑1471553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`