Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,486,862 C→G 16.4% A212A (GCC→GCG)  yhdZ → amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,486,8620CG16.4% 599.2 / 94.0 218A212A (GCC→GCG) yhdZamino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (97/85);  new base G (20/16);  total (117/101)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 224 total aligned reads displayed.

CGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGC  >  NC_002947/1486714‑1487008
                                                                                                                                                    |                                                                                                                                                  
cgTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGccc                                                                                                                                                   <  2:1742070/150‑1 (MQ=255)
  ttGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGATGGTGTCGCTCGCCGAGGATGGTATGACGATGATCTGTGTAACGCACGAGATGGGTTTTGCCCGt                                                                                                                                                 >  2:959341/1‑150 (MQ=12)
  ttGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGATGGTGTCGCTCGCCGAGGATGGTATGACGATGATCTGTGTAACGCACGAGATGGGTTTTGCCCGt                                                                                                                                                 >  2:4343883/1‑150 (MQ=12)
   tgtgCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGATGGTGTCGCTCGCCGAGGATGGTATGACGATGATCTGTGTAACGCACGAGATGGGTTTTGCCCGtc                                                                                                                                                >  2:1215123/1‑149 (MQ=12)
     tgCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTAcc                                                                                                                                              >  1:2400644/1‑150 (MQ=255)
        aTGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTg                                                                                                                                           >  1:1322586/1‑150 (MQ=255)
        aTGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGtcaggtt                                                                                                                                           >  2:3487455/1‑144 (MQ=14)
         tGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTgg                                                                                                                                          >  2:1848802/1‑150 (MQ=255)
         tGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTgg                                                                                                                                          <  2:5419962/150‑1 (MQ=255)
         tGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTgg                                                                                                                                          >  2:1606542/1‑150 (MQ=255)
             gCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGaa                                                                                                                                      <  2:2017418/150‑1 (MQ=255)
              cccAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAAc                                                                                                                                     >  2:2585275/1‑150 (MQ=255)
               ccAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAAcc                                                                                                                                    >  2:610802/1‑150 (MQ=255)
                cAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCg                                                                                                                                   <  2:3683626/150‑1 (MQ=255)
                 aaGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCgg                                                                                                                                  >  1:3125402/1‑150 (MQ=255)
                 aaGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCgg                                                                                                                                  <  2:4724801/150‑1 (MQ=255)
                 aaGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCgg                                                                                                                                  <  1:4826658/150‑1 (MQ=255)
                   gATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGt                                                                                                                                >  2:3934301/1‑150 (MQ=255)
                     tCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTa                                                                                                                              <  1:3370643/150‑1 (MQ=255)
                     tCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTa                                                                                                                              <  1:2548601/150‑1 (MQ=255)
                       aTGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGtcaggtt                                                                                                                                           >  1:5006736/1‑129 (MQ=17)
                       aTGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGtcaggtt                                                                                                                                           <  2:5006689/135‑7 (MQ=17)
                       aTGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATc                                                                                                                            <  2:5061104/150‑1 (MQ=255)
                           tGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCa                                                                                                                        >  2:2830835/1‑150 (MQ=255)
                           tGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCa                                                                                                                        <  2:4215809/150‑1 (MQ=255)
                            gTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCAt                                                                                                                       <  2:461046/150‑1 (MQ=255)
                            gTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCAt                                                                                                                       <  1:4286768/150‑1 (MQ=255)
                            gTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCAt                                                                                                                       <  2:4998634/150‑1 (MQ=255)
                             ttCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGACGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATg                                                                                                                      >  1:3479442/1‑150 (MQ=255)
                             ttCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATg                                                                                                                      >  1:1500273/1‑150 (MQ=255)
                                gacgaGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCGTGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGAc                                                                                                                   >  2:1893442/1‑150 (MQ=255)
                                gacgaGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGAc                                                                                                                   >  2:2754678/1‑150 (MQ=255)
                                 acgaGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATTGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGatc                                                                                                                  <  2:2287423/150‑3 (MQ=12)
                                   gtgCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAACGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAg                                                                                                                >  2:1449141/3‑150 (MQ=255)
                                   gaGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGatcag                                                                                                                >  1:2612579/1‑146 (MQ=12)
                                   gaGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGatcag                                                                                                                >  2:4456085/1‑146 (MQ=12)
                                   gaGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAg                                                                                                                >  2:1961732/1‑150 (MQ=255)
                                    aGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGatcagg                                                                                                               <  2:2992608/150‑6 (MQ=255)
                                    aGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAgg                                                                                                               <  2:1069578/150‑1 (MQ=255)
                                    aGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAgg                                                                                                               <  1:4758191/150‑1 (MQ=255)
                                     gCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGACCCGGGTTATCTTCATGGACAAggg                                                                                                              >  2:3038285/1‑150 (MQ=255)
                                      ccGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGATCAGGGc                                                                                                             <  1:4158773/150‑1 (MQ=255)
                                          ccTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGATCAGGGCcaga                                                                                                         <  1:4679217/150‑1 (MQ=255)
                                            tCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGAtt                                                                                                       >  2:2483813/1‑150 (MQ=255)
                                            tCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGAtt                                                                                                       >  1:2727218/1‑150 (MQ=255)
                                              ggCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATtgt                                                                                                     <  1:1077059/150‑1 (MQ=255)
                                               gCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATtgtg                                                                                                    <  2:2226378/150‑1 (MQ=255)
                                                 aCTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGa                                                                                                  <  1:3080033/150‑1 (MQ=255)
                                                   tGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGc                                                                                                <  2:395364/150‑1 (MQ=255)
                                                     gatccGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGATCAGGGCCAGATCGTTGAACAg                                                                                              >  2:1480009/4‑150 (MQ=255)
                                                     gatccGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGATCAGGGCCAGATCGTCGAACAg                                                                                              >  2:3786894/4‑150 (MQ=255)
                                                     gACCCGGAAATGGTCAAGGAGGTGCTGGAAACGTTGGCAGACCTGGCGCAGGACGGCATCACAATCATCTGCGTAACGCATGAAATGGGTTTTGCGCGCAAGATCGCCGATCGCGTCATCTTTATGGATCAGGGCAAGATCGTCGAggaa                                                                                              >  2:1759407/1‑147 (MQ=255)
                                                      atccGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGATCAGGGCCAGATCGTTGAACAga                                                                                             <  2:3319847/148‑2 (MQ=255)
                                                        ccGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGcc                                                                                           >  2:2917050/1‑150 (MQ=255)
                                                          ggAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGTCAGGTTGCCAATCGTGTGATCTTCATGGatcagg                                                                                                               <  2:2698444/128‑6 (MQ=255)
                                                          ggAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCcgc                                                                                         >  1:1745607/1‑150 (MQ=255)
                                                          ggAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCcgc                                                                                         <  2:1322586/150‑1 (MQ=255)
                                                             aaTGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGcc                                                                                      >  1:161881/1‑150 (MQ=255)
                                                             aaTGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGcc                                                                                      <  2:4720706/150‑1 (MQ=255)
                                                                 gTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGAt                                                                                  >  2:4385062/1‑150 (MQ=255)
                                                                 gTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGAt                                                                                  >  1:3720333/1‑150 (MQ=255)
                                                                  tCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATg                                                                                 >  1:3793011/1‑150 (MQ=255)
                                                                    aaGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGAc                                                                               <  1:2828245/150‑1 (MQ=255)
                                                                      ggAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGActt                                                                             >  2:1556243/1‑150 (MQ=255)
                                                                       gAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGActtc                                                                            <  2:1286812/150‑1 (MQ=255)
                                                                          gTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACttcttc                                                                         <  1:4184614/150‑1 (MQ=255)
                                                                           tGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCAGGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCg                                                                        <  2:2363109/150‑1 (MQ=255)
                                                                              tCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGAcc                                                                     <  2:1873028/150‑1 (MQ=255)
                                                                                gATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCgg                                                                   >  1:656841/1‑150 (MQ=255)
                                                                                        ggtggGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATtgtg                                                                                                    <  2:169865/109‑1 (MQ=255)
                                                                                        ggtggGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATtgtg                                                                                                    >  1:169865/1‑109 (MQ=255)
                                                                                         gtggGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGt                                                          >  1:2984623/1‑150 (MQ=255)
                                                                                         gtggGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGt                                                          >  1:2068065/1‑150 (MQ=255)
                                                                                                tGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGa                                                   <  1:4978776/150‑1 (MQ=255)
                                                                                                  gCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTTGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGAcc                                                 <  1:3870414/150‑1 (MQ=255)
                                                                                                    cGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCaa                                               >  1:3579926/1‑150 (MQ=255)
                                                                                                        gATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAgttg                                           <  2:4445459/150‑1 (MQ=255)
                                                                                                         aTGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGTCCGGCCGCGCAGTGACCGGACCAAgttgt                                          <  2:2068065/150‑1 (MQ=255)
                                                                                                         aTGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAgttgt                                          >  2:3147446/1‑150 (MQ=255)
                                                                                                         aTGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAgttgt                                          <  1:741987/150‑1 (MQ=255)
                                                                                                          tGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAgttgtt                                         <  1:198388/150‑1 (MQ=255)
                                                                                                             cATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACTAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCt                                      <  2:1578987/150‑1 (MQ=255)
                                                                                                             cATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCAGCCGCGCAGTGACCGGACCAAGTTGTTCCt                                      <  1:2917055/150‑1 (MQ=255)
                                                                                                                  ccATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGcc                                 <  1:1556243/150‑1 (MQ=255)
                                                                                                                  ccATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGcc                                 >  2:5208982/1‑150 (MQ=255)
                                                                                                                     tGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGa                              <  1:5147808/150‑1 (MQ=255)
                                                                                                                         ctGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCt                          <  1:5208982/150‑1 (MQ=255)
                                                                                                                           gCGTGACCCACGAATTGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGc                        <  2:3824374/150‑1 (MQ=255)
                                                                                                                            cGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCg                                                                    >  1:4457171/1‑105 (MQ=255)
                                                                                                                             gTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCAt                      <  1:4603006/150‑1 (MQ=255)
                                                                                                                               gACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTg                    <  2:4475805/150‑1 (MQ=255)
                                                                                                                                  ccACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATg                 >  2:815162/1‑150 (MQ=255)
                                                                                                                                  ccACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATg                 >  1:1753745/1‑150 (MQ=255)
                                                                                                                                  ccACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATg                 >  2:5142154/1‑150 (MQ=255)
                                                                                                                                  ccACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATg                 >  2:1117834/1‑150 (MQ=255)
                                                                                                                                  ccACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATg                 >  2:2128933/1‑150 (MQ=255)
                                                                                                                                        aaTGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGttt           <  1:1284246/150‑1 (MQ=255)
                                                                                                                                             gCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTg          >  1:228383/1‑146 (MQ=255)
                                                                                                                                               tttGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTgc    >  1:3902269/1‑150 (MQ=255)
                                                                                                                                               tttGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTgc    <  2:2727218/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATGGACAAGGGCGAGATTGTGGAGCAGGCCGCGCCGGATGACTTCTTCGACCGGCCGCGCAGTGACCGGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGC  >  NC_002947/1486714‑1487008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑