Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,253,0230CG54.5% 2.7 / 16.0 12A375P (GCG→CCG) nupXnucleoside permease
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base G (6/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAAT  >  NC_000913/2252968‑2253080
                                                       |                                                         
cGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGGCCCcaccaccac                                               >  1:33153/1‑68 (MQ=255)
 gTAAACCAAGCTGGGCGATTTCCGGCGCACTGTGTGGCGCAACCGCAGAAAACGCCCCCaccaccacc                                              <  1:6117/68‑1 (MQ=255)
  tAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGAGCCCACCACCAccc                                             >  1:733264/1‑68 (MQ=255)
          gCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGaa                                     <  1:912013/68‑1 (MQ=255)
                cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAg                               <  1:828554/68‑1 (MQ=255)
                             caggTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGc                  <  1:649778/66‑1 (MQ=255)
                                  gtgGCGCAACCGCAGAAACGGGCCCCACCACCACCCCGATAGAACCAAAGTTAGCGCAACCGCACAAc             >  1:454076/1‑68 (MQ=38)
                                       gcAACCGCAGACACCGGCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa        >  1:75735/1‑68 (MQ=38)
                                       gcAACCGCAGAAACGGGGCCCACCACCACCCCGATAGGACCAAAGTTAGCGAAACCGCACACcgccgg        >  2:649780/1‑65 (MQ=25)
                                       gcAACCGCAGAAAAGGGCCCCACCACCGCCCCGATAGGACCAAAGTTAGCGAAACCGCACAACGCGaa        >  2:892781/1‑68 (MQ=38)
                                       gcAACCGCAGAAAAGGGCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACACCGCGaa        >  1:280579/1‑68 (MQ=38)
                                             gCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAaat  <  1:295825/68‑1 (MQ=255)
                                                       |                                                         
CGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAAT  >  NC_000913/2252968‑2253080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑