Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	129.2 / NA	38	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/15);  total (23/15)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588                                                                  |                                                                gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                               >  2:319811/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCAAATCCgg                                                             >  2:762621/1‑68 (MQ=255)        gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTaa                                                        >  2:241084/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  1:476800/1‑68 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcag                                                  >  1:456243/1‑66 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  1:295837/68‑1 (MQ=255)              gcgGTTAAAAATAAAAGGCATAACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  >  1:610246/1‑68 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                                        >  1:299495/1‑68 (MQ=255)                             aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   >  2:536024/1‑68 (MQ=255)                             aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   <  2:498190/68‑1 (MQ=255)                             aGGCATCACGGTGGTGAATAATGCAGCTTTCGGCCAATCCGGATAAATCGCCGTAAGTTCAGCGCGAg                                   >  2:232765/1‑68 (MQ=255)                               gCATCACGGTGGTGAATAATGCCGATTTCGGCAAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCa                                 >  2:189355/1‑68 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCATTCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:189355/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:299496/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:833624/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:832566/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:476802/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:319809/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTCTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:614045/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:375742/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGGCTATTCAGCGCGAGCAGACt                             <  1:377088/68‑1 (MQ=255)                                         ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                       >  1:429089/1‑68 (MQ=255)                                         ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                       >  2:290044/1‑68 (MQ=255)                                              ataatGCCGCTTTCTGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  1:486010/1‑68 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  2:252542/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   <  1:180615/49‑1 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   >  2:180615/1‑49 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                <  1:895836/68‑1 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                >  1:566396/1‑68 (MQ=255)                                                aatGCAGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCagc                >  2:835483/1‑66 (MQ=255)                                                  tGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCTCAACCt              <  2:908759/68‑1 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:16622/1‑68 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:891723/1‑68 (MQ=255)                                                    cctcTTTCGTCCAATCCGTATAAATCGCCGCCAATTCAGCTCTAGCAGACTCATATTGAGCAACCTcc            <  1:660940/65‑1 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc           >  2:808214/1‑68 (MQ=255)                                                         ttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCgcg       >  1:522338/1‑66 (MQ=255)                                                             gCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc   >  1:654081/1‑68 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGCGTCCcg  >  2:822357/1‑68 (MQ=255)                                                                  |                                                                GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑