Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,956	A→C	T63P (ACG→CCG)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,956	0	A	C	100.0%	89.0 / NA	25	T63P (ACG→CCG)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/20);  total (5/20)

AGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATC  >  NC_000913/2810894‑2811020                                                               |                                                                 agaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTg                                                             >  1:778323/1‑68 (MQ=255)          aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAg                                                    <  2:289653/68‑1 (MQ=255)              aaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGaaa                                                <  2:53681/68‑1 (MQ=255)                 gCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAAcc                                             >  2:879172/1‑68 (MQ=255)                   tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACcac                                           <  2:501113/68‑1 (MQ=255)                      aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt                                        <  2:778325/68‑1 (MQ=255)                      aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt                                        <  2:638443/68‑1 (MQ=255)                      aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt                                        <  1:926960/68‑1 (MQ=255)                              gggATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGcc                                >  2:855669/1‑68 (MQ=255)                                    aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa                          <  1:137082/68‑1 (MQ=255)                                    aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa                          <  2:93409/68‑1 (MQ=255)                                    aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa                          <  1:147677/68‑1 (MQ=255)                                                tttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGc              <  2:418409/68‑1 (MQ=255)                                                 ttATGTCGTTGATTCCGCTTGAGTCTCAGGAATACCACAGCATTCAGCCTTCTGAATTAAGTTGTGct             <  2:411286/68‑1 (MQ=255)                                                 ttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             <  2:690525/68‑1 (MQ=255)                                                 ttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             <  2:739140/68‑1 (MQ=255)                                                 ttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             <  2:744746/68‑1 (MQ=255)                                                 ttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             <  1:879169/68‑1 (MQ=255)                                                      gCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             >  1:891785/1‑63 (MQ=255)                                                      gCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct             <  2:891788/63‑1 (MQ=255)                                                      gCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTgg        >  2:20611/1‑68 (MQ=255)                                                      gCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTgg        <  1:127819/68‑1 (MQ=255)                                                      gCGGTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTgg        <  2:791559/68‑1 (MQ=255)                                                           gATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGatcat   <  1:20611/68‑1 (MQ=255)                                                            aTTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGatcatc  <  2:111064/68‑1 (MQ=255)                                                               |                                                                 AGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATC  >  NC_000913/2810894‑2811020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑