Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	36.9 / NA	11	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

TAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAA  >  NC_000913/3413522‑3413628                                                  |                                                          tAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                         <  1:104478/68‑1 (MQ=255)   aTCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAataaataa                                       <  2:169616/68‑1 (MQ=255)    tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                      <  1:419985/68‑1 (MQ=255)    tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                      <  2:494793/68‑1 (MQ=255)      tATCACCTAATGTGTATTTATACAAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatat                                    <  2:349629/68‑1 (MQ=255)                        tataCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGAt                  >  2:557610/1‑68 (MQ=255)                            cGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt              <  1:91847/68‑1 (MQ=255)                                   cTAATATTGAGTTGCCATAAATCGTTAAataaataa                                       >  1:771183/1‑36 (MQ=255)                                   cTAATATTGAGTTGCCATAAATCGTTAAataaataa                                       <  2:771185/36‑1 (MQ=255)                                        atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa  >  1:3355/1‑68 (MQ=255)                                        atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa  >  1:876550/1‑68 (MQ=255)                                                  |                                                          TAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAA  >  NC_000913/3413522‑3413628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑