Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	98.7 / NA	31	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/14);  total (17/14)

GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264036‑264161                                                               |                                                                gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                            >  2:912814/1‑68 (MQ=255) gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                            >  1:899962/1‑68 (MQ=255) gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                            >  1:696594/1‑68 (MQ=255)  tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACt                                                           <  2:505886/68‑1 (MQ=255)      gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                       >  2:594378/1‑68 (MQ=255)           tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                  >  2:389811/1‑68 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                 <  1:736696/68‑1 (MQ=255)             aggcaggTATAGGGCCCATTGCGGTCCTGGCAGCGTTCGGCGTATACCGCTAAGACTCCGGCGATATc                                                >  1:747214/1‑68 (MQ=38)                  ggTATAGGGCCAATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                           >  1:774936/1‑68 (MQ=255)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGACCGt                                        >  1:892966/1‑68 (MQ=255)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                        <  1:167892/68‑1 (MQ=255)                          gCCCATTGCGGTCGTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:684855/1‑68 (MQ=25)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  1:798408/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  1:19068/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:24796/1‑68 (MQ=255)                            ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGcc                                 <  1:663198/68‑1 (MQ=255)                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                              <  1:335330/68‑1 (MQ=255)                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                              <  1:750290/68‑1 (MQ=255)                               ttGCGGGCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                              <  1:592797/68‑1 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                             <  1:110273/68‑1 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                             <  1:119431/68‑1 (MQ=255)                                   ggTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                          <  2:798410/68‑1 (MQ=255)                                     tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg                        <  2:531065/68‑1 (MQ=255)                                           cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                  >  2:677898/1‑68 (MQ=255)                                              ctttCGGCGGATACCGCTAGGTCTCCGGCGATATCCTGATCGTCCCTGCCGGTGTACTGACAGACGCt               <  1:922415/66‑1 (MQ=18)                                                ttCGTCGTATACCTCTAGTACGCCGGCTATTTCCGGAACGTCCCTGCCGGTGTAATGACAGTCGCTAc             <  1:259215/68‑1 (MQ=17)                                                   ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt          <  1:115521/68‑1 (MQ=255)                                                        ataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc     >  1:857236/1‑68 (MQ=255)                                                           ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGCCAGACGCTACCGTGCCACTgg  >  1:269534/1‑68 (MQ=255)                                                           ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg  >  1:172558/1‑68 (MQ=255)                                                           ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGAGCCACTgg  >  2:699701/1‑68 (MQ=255)                                                               |                                                                GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264036‑264161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑