| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,220 | 0 | C | T | 91.7% | 15.3 / ‑3.3 | 12 | H366H (CAC→CAT) | icd | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (8/3); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
TATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGG > NC_000913/1196155‑1196267 | tatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCatat < 1:913364/68‑1 (MQ=255) gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGc > 1:213322/1‑68 (MQ=255) tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc > 2:166983/1‑67 (MQ=21) tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTAGGACTGAAGCGGCTGAcc > 1:199383/1‑67 (MQ=21) ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTg > 1:764455/1‑68 (MQ=17) ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTg > 1:84626/1‑68 (MQ=17) attCTCTCCGCTGAGATGATGCTGCGCCATTTGGGTTGGACTGAAGCGGCTGACCTGATTGTTTAAgg < 2:312897/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg < 1:270387/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg < 1:802483/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg > 2:95510/1‑68 (MQ=14) tctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:177012/1‑68 (MQ=11) tctctcCGCTGAGATGATGCTGCGCAATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 2:139642/1‑68 (MQ=11) gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAgg < 1:165184/68‑1 (MQ=255) | TATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGG > NC_000913/1196155‑1196267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |