Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	49.7 / NA	20	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/16);  total (4/16)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCA  >  NC_000913/2679460‑2679572                                                                  |                                                gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGACGCTTTCGGCCAATcc                                               >  2:671822/1‑68 (MQ=255)    tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                            <  1:374143/68‑1 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCACTCCGGATTAATCGCCg                                  >  2:352540/1‑68 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                  <  1:191616/68‑1 (MQ=255)                               gcttCATGGTGGTGATTAATGTCGCTTTCGGCTAATCCGGATTAATCGCCGTCAATTCAGCGCTAGCa                 <  1:66389/65‑1 (MQ=255)                                 aTCACGGTTGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  1:139552/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  1:332407/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  1:456185/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  1:289176/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  2:32536/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  1:181922/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  2:472499/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  2:515478/68‑1 (MQ=255)                                 aTCACGGTGGCGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa               <  2:251374/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt             <  2:396570/68‑1 (MQ=255)                                         ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt       <  2:450350/68‑1 (MQ=255)                                           tgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc     <  2:183182/68‑1 (MQ=255)                                              ataatGCCGCTTTCGGCCAGTCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgcg  >  2:365916/1‑67 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt             <  2:102545/57‑1 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt             >  1:102545/1‑57 (MQ=255)                                                                  |                                                GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCA  >  NC_000913/2679460‑2679572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑