| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,810,956 | A→C | T63P (ACG→CCG) | mprA → | transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,810,956 | 0 | A | C | 100.0% | 80.4 / NA | 22 | T63P (ACG→CCG) | mprA | transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/13); total (9/13) | |||||||||||
AGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCAT > NC_000913/2810894‑2811019 | agaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTg > 1:450755/1‑68 (MQ=255) ccGCATTAAAATGCTGATGGCTCTGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGt < 1:217144/68‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGtc > 2:356891/1‑68 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGtct < 1:2850/68‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGa > 1:308553/1‑68 (MQ=255) aaaaTGCTGAAGGATCAGGGGATTAACGAGACGATGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGa > 2:405221/1‑68 (MQ=255) aaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGaaa > 1:509152/1‑68 (MQ=255) gCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAAcc < 2:47852/68‑1 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCATGAAAACcac < 2:406442/68‑1 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt < 1:199292/68‑1 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt < 1:405221/68‑1 (MQ=255) aGGCGCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGt < 1:409428/68‑1 (MQ=255) gggATTAACGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGcc > 1:162137/1‑68 (MQ=255) aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa < 2:573012/68‑1 (MQ=255) aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa < 2:352168/68‑1 (MQ=255) aaCGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGa > 1:350774/1‑68 (MQ=255) cGAGACGTTGTTTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAAt > 2:334826/1‑68 (MQ=255) gttgttTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTg > 2:595802/1‑59 (MQ=255) gttgttTATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTg < 1:595801/59‑1 (MQ=255) ttATGGCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGct < 1:498230/68‑1 (MQ=255) gCGTTGATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTgg < 2:509152/68‑1 (MQ=255) gATTCCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGatcat < 1:904/68‑1 (MQ=255) | AGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCAT > NC_000913/2810894‑2811019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |