Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	59.8 / NA	17	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/8);  total (9/8)

TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATC  >  NC_000913/3413512‑3413626                                                            |                                                        tAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCg                                                 >  1:233657/1‑68 (MQ=255) tAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCg                                                 >  1:559831/1‑68 (MQ=255)         gTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                         <  2:515371/68‑1 (MQ=255)         gTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                         <  1:85299/68‑1 (MQ=255)           tAATCTATCACCTAATGTGTATTTATACGAGATGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                       <  2:4059/68‑1 (MQ=255)           tAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                       <  2:634155/68‑1 (MQ=255)               cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                   >  1:465580/1‑68 (MQ=255)                            tgtATATATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATAATATATAca                      >  2:468986/1‑67 (MQ=255)                                    taCGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                         >  1:530316/1‑41 (MQ=255)                                    taCGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                         <  2:530316/41‑1 (MQ=255)                                    taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc              >  1:102808/1‑68 (MQ=255)                                      cGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt            >  2:281859/1‑68 (MQ=255)                                      cGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt            >  2:308109/1‑68 (MQ=255)                                        agagGCTAATAATGAGATGCCATAAATCGTGAAATAAATAATATATATTATTTAACTAAGATAAAtta          >  2:178414/1‑67 (MQ=25)                                         gagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa         <  2:465580/68‑1 (MQ=255)                                                atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc  <  2:102808/68‑1 (MQ=255)                                                atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc  <  2:233657/68‑1 (MQ=255)                                                            |                                                        TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATC  >  NC_000913/3413512‑3413626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑