| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,041,386 | 0 | G | C | 26.1% | 29.3 / 12.0 | 23 | G257A (GGA→GCA) | appA | phosphoanhydride phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (10/7); new base C (0/6); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAATT > NC_000913/1041326‑1041443 | cAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATc < 2:228441/68‑1 (MQ=255) gagaTATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTcaca < 1:326826/68‑1 (MQ=255) gagaTATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTcaca < 1:866921/68‑1 (MQ=255) atatTTCTCCTGCAACAGGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCa < 1:510465/68‑1 (MQ=255) atTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGTGTGGGCAAGGATCACCGATTCACACCAGt < 2:969063/68‑1 (MQ=255) atTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGt < 1:558954/68‑1 (MQ=255) atTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTCACACCAGt < 2:882305/68‑1 (MQ=255) tctcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGa < 2:698726/68‑1 (MQ=255) ctcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGaa > 2:593302/1‑68 (MQ=255) ctcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATAACCGATTCACACCAGTGGaa > 2:591957/1‑68 (MQ=255) tcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCa < 1:361330/61‑1 (MQ=255) tcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCa > 2:361330/1‑61 (MQ=255) ccTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAaca > 2:192730/1‑68 (MQ=255) ccTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAaca > 1:918414/1‑68 (MQ=255) aacaaGCACAGGGAATGCCGGAGCCGGGGCGGGCAAGGATCACCGATTCACACCAGTGGAACACCTTg < 2:270033/68‑1 (MQ=255) aacaaGCACAGGGAATGCCGGAGACGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTg > 2:848961/1‑68 (MQ=255) gCACAGGGAATGCCGGAGCCGGGGTGGGGACGGATCACCGATTCACACCAGTGGAACACCTTGCTAAg < 1:602998/68‑1 (MQ=255) gAATGCCGGAGCCGGGGTGGGGTAGGATCACCGATGCACACAAGTGGAACACCTGGCTAGGTTTGCAt > 2:377128/1‑68 (MQ=255) gAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACCCCAGTGGAACACCTTGCTAAGTTTGCAt > 1:419067/1‑68 (MQ=255) tGCCGGAGCCGTGGTTGCGTAGGATCACCGATTCACACCAGTGGATCACCTTGCTACGGTTGCATAAc < 1:737874/68‑1 (MQ=255) gCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACAGCTTGCTAAGTTTGCATAACGCGCAAt > 1:795804/1‑68 (MQ=255) gCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAAt < 2:297251/68‑1 (MQ=255) ccGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAAtt > 1:857576/1‑68 (MQ=255) | CAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAATT > NC_000913/1041326‑1041443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |