| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,747,168 | 0 | G | T | 35.7% | 45.5 / 10.4 | 28 | G13V (GGG→GTG) | ydhS | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (10/8); new base T (0/10); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGC > NC_000913/1747103‑1747234 | tCGAATGAATGCGTACAGGTAAGTAACAATGAAAAAATTTGTTATTTTTGTTTCGGGGACTACGGTGa < 1:727720/68‑1 (MQ=255)tCGAAAGAATGCGTACATGTAAGTAACTATGAATAAAATTGCTATTGTGGGTTCCTGTCCTTCGGGGa < 2:513877/68‑1 (MQ=255)tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTGTTATTGTGGTTTCGGGGACTACGGTGa < 1:965717/68‑1 (MQ=255)tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa < 2:30418/68‑1 (MQ=255)tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa < 2:150535/68‑1 (MQ=255) aGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa < 2:492300/63‑1 (MQ=255) aGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa > 1:492300/1‑63 (MQ=255) aTGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTAGGTTTCGGGGACTACGGTGATCTACAcc < 1:441731/68‑1 (MQ=255) aTGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc < 2:252477/68‑1 (MQ=255) aTGCGTACAGGTAAGTAACAATGAAAAAAATTGCAATTGTGGGTGCCGGGCCTACGGGGATCTACAcc < 2:636483/68‑1 (MQ=255) gCGTACAGGTTAGTAACAATGAAAAAAATTTCTTTTGTTGTTTCGGGGACTACGGTGATCTACACCtt < 1:780756/68‑1 (MQ=255) ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc > 1:585004/1‑59 (MQ=255) ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAcc < 2:585004/59‑1 (MQ=255) ttagttaCTATGAATAAAATTGTTTTTGTTGTTTCGGGGTCTACGGTGAGCTACACCTTATTCTCGCt < 1:849772/66‑1 (MQ=255) gtaaCAATGAAAAAATTTGTTATTTTGGTTTCCGGGACTACGGTGATCTACACCTTATTCTCGCTTCt < 1:211237/68‑1 (MQ=255) gtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCt > 1:560799/1‑68 (MQ=255) gtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCt > 1:1015172/1‑68 (MQ=255) aaCAATGAAAAAAATTGCTATTGTGGGTGCCGCGCCTACGGGGATCTACACCTTATTCTCGCTTCTAc > 2:344868/1‑68 (MQ=255) aaCAATGAAAAAAAATGCTAATGTGGGTGCCGGGCCTACGGGGATCTACACCATATTCTCGCTTCTAc > 2:618737/1‑68 (MQ=255) aaaaaaTTGCTTTTGTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGcaacaa < 1:681720/68‑1 (MQ=255) aTTGCTATTGTGGGGGCCGGGCCTACGGTGATCTACTCCTTATTCTCGCTTCTACAGCTACAAACTcc < 2:586656/68‑1 (MQ=255) ttGCTATTTTGGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa < 1:121956/68‑1 (MQ=255) tATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACttt < 2:347782/68‑1 (MQ=255) gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttct > 2:441566/1‑68 (MQ=255) gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttct > 2:718798/1‑68 (MQ=255) ccGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATc > 2:112883/1‑68 (MQ=255) gCCTGCGGGGATCTACGCCTTATTCTCGCTTCTACAGCAACGAACTCCACTTTCTATTTCTATCATCg > 1:872472/1‑68 (MQ=255) cGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg < 1:308341/68‑1 (MQ=255) ggggATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAgg < 2:280364/68‑1 (MQ=255) gggATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGc > 2:290976/1‑68 (MQ=255) | TCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGC > NC_000913/1747103‑1747234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |