| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,253,023 | 0 | C | G | 55.6% | 2.3 / 26.7 | 18 | A375P (GCG→CCG) | nupX | nucleoside permease |
| Reads supporting (aligned to +/- strand): ref base C (1/7); new base G (10/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACA > NC_000913/2252961‑2253090 | cAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCcca < 2:350443/68‑1 (MQ=255) gcgcCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccacc < 1:917301/68‑1 (MQ=255) aCCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAACGGGGCCCCCCACAACCCCGa > 2:109366/1‑68 (MQ=21) aCCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGa < 1:394262/68‑1 (MQ=255) gATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGt < 2:861883/68‑1 (MQ=255) ggCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGaa < 1:708225/68‑1 (MQ=255) gtgGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAc < 2:645587/68‑1 (MQ=255) gcAACCGCAGACAACGCCCCCACCACAACCCCGAAAGACCCAAAGTTAGCGAAACCACCCGACGCGaa > 2:575392/1‑68 (MQ=25) gcAACCGCAGAAAGGGGGCCCACCCCCACCCCGATAGGACCAAAGTTAGCGAAACCGGACCAagcgga > 2:731828/1‑66 (MQ=16) gcAACCGCAGAAACGGGGCCCACCCCCCCCCCGATAGGACCAAAGTTAGCGAAACCGCACAACGCGaa > 1:1003334/1‑68 (MQ=25) gcAACCGCAGAAACGGGGCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACACcgcgga > 2:238015/1‑66 (MQ=25) gcAACCGCAGAAAAGGGGCCCACCACCACCCCGATAGGACCAAAGTTAGCGAAACCGCACAAcgcgga > 2:650060/1‑66 (MQ=37) gcAACCGCAGAAAAGGGCCCCACCCCCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa > 1:1026988/1‑68 (MQ=38) gcAACCGCAGAAAACGGGCCCACCACCACCCCGATAGGACCAAAGTTTGAGAAACCGCACAACGCGaa > 2:774547/1‑68 (MQ=255) gcAACCGCAGAAAACGGCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa > 2:128405/1‑68 (MQ=255) gcAACCGCAGAAAAAGGCCCCCCCACCACCCCGATAGAACCAAAGTTAGCGAACCCGCACAACGCGaa > 1:473818/1‑68 (MQ=38) gaCCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGTAgg < 2:590876/67‑1 (MQ=255) aaCCGCAGAAAACGGGCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAgg > 2:43444/1‑68 (MQ=255) cccccACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCcaca < 1:221673/68‑1 (MQ=255) | CAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACA > NC_000913/2252961‑2253090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |