| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 264,098 | G→T | L50L (CTC→CTA) | ykfH ← | uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 264,098 | 0 | G | T | 96.3% | 82.5 / NA | 27 | L50L (CTC→CTA) | ykfH | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (16/10); minor base A (1/0); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG > NC_000913/264036‑264161 | gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc > 2:824497/1‑68 (MQ=255)gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc > 1:796880/1‑68 (MQ=255)gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGAGTTCGGCGTATACCGCTAGGAc > 2:971455/1‑68 (MQ=255) tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACt < 1:984032/68‑1 (MQ=255) atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACAGCTAGGACTCCGGCGat > 2:370935/1‑68 (MQ=255) caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat < 2:571087/68‑1 (MQ=255) caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg > 1:1040037/1‑68 (MQ=255) caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAAGACTCCGGCGATATCAgg > 2:413680/1‑68 (MQ=37) ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt > 2:238428/1‑68 (MQ=255) ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt > 2:91435/1‑68 (MQ=255) gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCAAGGACTCCGGCGATATCCGGAACGTCCCtc > 1:479221/1‑67 (MQ=255) ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat < 1:893572/52‑1 (MQ=255) ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat > 2:893572/1‑52 (MQ=255) ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt < 2:159694/68‑1 (MQ=255) ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt < 1:596375/68‑1 (MQ=255) ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGgt < 1:824497/68‑1 (MQ=255) tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATAACCGGATCGTCCCTGCCGgtg < 2:672103/68‑1 (MQ=21) tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg > 1:616282/1‑68 (MQ=255) ccTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa > 1:147491/1‑68 (MQ=255) cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc > 2:131660/1‑68 (MQ=255) cGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGc < 1:468303/68‑1 (MQ=255) gTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc < 1:642862/68‑1 (MQ=255) aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg > 1:492317/1‑68 (MQ=255) aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg > 1:361225/1‑68 (MQ=255) ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc > 1:85301/1‑57 (MQ=255) ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc < 2:85301/57‑1 (MQ=255) ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg > 1:77078/1‑68 (MQ=255) | GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG > NC_000913/264036‑264161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |