Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,502	C→T	G11R (GGA→AGA)	ymfD ←	e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,502	0	C	T	100.0%	101.1 / NA	32	G11R (GGA→AGA)	ymfD	e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/21);  total (11/21)

CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTT  >  NC_000913/1197437‑1197566                                                                  |                                                                 cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                <  2:70611/68‑1 (MQ=255) cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                <  1:250350/68‑1 (MQ=255) cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                <  1:666142/68‑1 (MQ=255)  tCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCtgtg                                                               <  2:663781/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                              <  1:17871/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                              <  1:39813/68‑1 (MQ=255)     ctaaGATATCTAGAGGGCATCGTATGAGGTTGTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                                            <  1:454686/66‑1 (MQ=255)     cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                                            <  1:9514/68‑1 (MQ=255)        aGATATCTAGAGGGCTTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                         <  2:682686/68‑1 (MQ=255)             tCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGt                                                    <  2:1020206/68‑1 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa                                               >  2:232604/1‑68 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                <  2:495518/50‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                                >  1:495518/1‑50 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCata                                                           <  2:1511/55‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCata                                                           >  1:1511/1‑55 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                              <  1:668000/68‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                              <  1:232604/68‑1 (MQ=255)                     gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCa                                            >  1:407498/1‑68 (MQ=255)                     gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCa                                            >  1:387402/1‑68 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                                 <  1:476308/68‑1 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                                 <  2:407498/68‑1 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                                 <  2:515354/68‑1 (MQ=255)                                 gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa                                <  2:596695/68‑1 (MQ=255)                                      gctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                           <  2:387402/68‑1 (MQ=255)                                        tgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGt                         <  2:471549/68‑1 (MQ=255)                                            ttCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAtt                     <  1:345311/68‑1 (MQ=255)                                                ctGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTTATGTTATTTTTa                 >  2:809266/1‑68 (MQ=255)                                                     cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGaa            >  2:555137/1‑68 (MQ=255)                                                              aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa                                >  1:383026/1‑39 (MQ=255)                                                              aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa                                <  2:383026/39‑1 (MQ=255)                                                              aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttt   >  2:574489/1‑68 (MQ=255)                                                              aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttt   >  2:73384/1‑68 (MQ=255)                                                              aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttt   >  1:582526/1‑68 (MQ=255)                                                               ctctGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCtttt  >  2:545236/1‑68 (MQ=255)                                                                  |                                                                 CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTT  >  NC_000913/1197437‑1197566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑