Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,035	C→T	T89M (ACG→ATG)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,035	0	C	T	100.0%	50.5 / NA	16	T89M (ACG→ATG)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/3);  total (13/3)

ACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATC  >  NC_000913/2810978‑2811097                                                          |                                                               acaGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGcc                                                      <  1:341141/68‑1 (MQ=255)           gCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTgg                                            >  2:54956/1‑68 (MQ=255)             cTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGaa                                          >  1:504000/1‑68 (MQ=255)                         gtagtgCTCTTGGATCATCACGTACCAACGAGATGCGTATTGACGATGAACTGGAAAAACGAGGTTgg                              >  1:748803/4‑68 (MQ=37)                         gTTGTGCTCTTGGATCATCCCGTACCTACGCGATGCGTATTGCCGATGAACTGGAAAGACGCGGTAgg                              >  2:957600/1‑68 (MQ=255)                               ctctTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGaa                        <  2:1019324/68‑1 (MQ=255)                                      atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGGACGTCGTg                 >  1:649108/1‑68 (MQ=255)                                      atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTg                 >  1:707725/1‑68 (MQ=255)                                      atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTg                 >  1:788419/1‑68 (MQ=255)                                      atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTg                 >  1:347330/1‑68 (MQ=255)                                      atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTg                 >  2:608696/1‑68 (MQ=255)                                           cccGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGc            >  1:645008/1‑68 (MQ=255)                                                aCCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATaa       <  2:707725/68‑1 (MQ=255)                                                 ccAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAAc      >  1:668438/1‑68 (MQ=255)                                                 ccAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAAc      >  1:419807/1‑68 (MQ=255)                                                 ccAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAAc      >  1:134191/1‑68 (MQ=255)                                                     cgcgatgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATc  >  1:546991/1‑68 (MQ=255)                                                     cgcgatgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATc  <  1:750367/68‑1 (MQ=255)                                                          |                                                               ACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATC  >  NC_000913/2810978‑2811097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑