Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	58.3 / NA	17	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/12);  total (5/12)

AGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATATA  >  NC_000913/3413518‑3413634                                                      |                                                                aGGTTAATCTATCACCTAATGTGTATTAATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaat                                                   <  2:255783/68‑1 (MQ=255)   gTTAATCTATCACCTACTGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                                 <  1:418656/68‑1 (MQ=255)        tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                            <  2:182302/68‑1 (MQ=255)        tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                            <  1:10775/68‑1 (MQ=255)         cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                           <  2:44875/68‑1 (MQ=255)                cTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATAtatta                                    >  2:313784/1‑68 (MQ=255)                    tgtgTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTa                                >  2:1026319/1‑68 (MQ=255)                     gtgtATTTATACGAGAGGCTATTATTGAGTTGCCAAAAATCGTTAAATAAATAATATATATTATTTAc                               <  2:630394/68‑1 (MQ=38)                       gtATTTATACGAGAGTCTAATATTGAGTTGCCATAAATCGTTAAATATATAATATATATTATTTACCt                             <  1:625837/68‑1 (MQ=255)                           ttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGa                         <  2:579481/68‑1 (MQ=255)                              taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                      >  1:85135/1‑68 (MQ=255)                                cGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt                    >  1:494350/1‑68 (MQ=255)                                    agGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCAc                >  1:638693/1‑68 (MQ=255)                                          atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc          <  1:530167/68‑1 (MQ=255)                                          atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc          <  2:638693/68‑1 (MQ=255)                                             ttGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAat       <  1:714408/68‑1 (MQ=255)                                                  ttGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatatata  <  2:85135/68‑1 (MQ=255)                                                      |                                                                AGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATATA  >  NC_000913/3413518‑3413634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑