| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,984,188 | 0 | C | G | 70.6% | 11.5 / 13.7 | 17 | G294G (GGG→GGC) | araG | L‑arabinose ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base G (12/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTAC > NC_000913/1984124‑1984255 | cGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcggccc > 2:715446/1‑68 (MQ=255) atatAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcgccccgc < 2:761451/68‑1 (MQ=255) atatAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcgccccgc < 1:656192/68‑1 (MQ=255) atatAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcgccccgc < 1:715445/68‑1 (MQ=255) atatAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcgccccgc < 2:330949/68‑1 (MQ=255) atatAAACCTGACCGGCGGTGAGTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAcgccccgc < 1:709602/68‑1 (MQ=255) ccTGGCCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAAATCGCTACGGCCCGCCCCTagc > 1:571161/1‑66 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGCCCGCCCCTAcc > 2:695808/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGCCCGCCCCTAcc > 1:651346/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGCCCGCCCCTAcc > 1:708203/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGCCCGCCCCTAcc > 2:59949/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGCCCGCCCCTAcc > 2:360009/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGGACCGCCACTAcc > 2:525433/1‑68 (MQ=255) ccTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTAAGGCCCGCCCCTAcc > 2:690389/1‑68 (MQ=255) tgtttttCGTCCCGACAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGc < 1:230004/65‑1 (MQ=255) tGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGc < 2:655188/68‑1 (MQ=255) ccTTTCATTAATTCGCTACGGCCCGCCCCTACCAGACCAAACAGCCcaacaa > 2:582852/1‑52 (MQ=255) ccTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCcaacaa < 1:582852/52‑1 (MQ=255) ttAATTCGCTAGGGCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGa > 2:187021/1‑68 (MQ=255) aaTTCGCTACGGCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGGCt > 1:366020/1‑68 (MQ=255) ttCGCTACGGCCCGCCCCTACCAGACCAAACAGCCCAACAATTTcaccac > 2:460776/1‑50 (MQ=255) ttCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTcaccac < 1:460776/50‑1 (MQ=255) cTACTCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCTAACCGCCAGACTTATTgg < 2:228756/68‑1 (MQ=255) ccccgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTac < 1:187021/68‑1 (MQ=255) | CGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTAC > NC_000913/1984124‑1984255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |