Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	67.2 / NA	22	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/9);  total (13/9)

TGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264037‑264161                                                              |                                                                tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACt                                                           <  2:455107/68‑1 (MQ=255)     gCTCATCAGGCAGGTTTAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                       >  1:545480/1‑68 (MQ=255)        catcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa                                                    <  1:196359/68‑1 (MQ=255)             ggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACAGCTAGGACTCCGGCGATATcc                                               >  2:143095/1‑68 (MQ=255)                         gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:677438/1‑68 (MQ=255)                         gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  1:415947/1‑68 (MQ=255)                         gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:56467/1‑68 (MQ=255)                         gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:258821/1‑68 (MQ=255)                         gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGAGATCCGGAACGTCCCTg                                   >  2:199532/1‑68 (MQ=255)                           ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGcc                                 >  2:800547/1‑68 (MQ=255)                                 cGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTa                           <  1:791772/68‑1 (MQ=255)                                    tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg                        >  2:438329/1‑68 (MQ=255)                                    tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCAGGTGTAATg                        >  1:666433/1‑68 (MQ=255)                                        ggcggcGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAg                    <  1:56467/68‑1 (MQ=255)                                        ggcggcGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAg                    <  2:89280/68‑1 (MQ=255)                                             cgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt               <  2:406778/68‑1 (MQ=255)                                                    cGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGc        <  1:119411/68‑1 (MQ=255)                                                      tataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCa      <  2:744754/68‑1 (MQ=255)                                                       ataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc     <  2:415947/68‑1 (MQ=255)                                                         aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCCGAGAAATGACAGACGCTACCGTGCCACTg   >  1:717708/1‑68 (MQ=17)                                                          ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGCCGCTACCGTGCCACTgg  >  1:197274/1‑68 (MQ=255)                                                          ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg  >  1:694791/1‑68 (MQ=255)                                                              |                                                                TGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264037‑264161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑