| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,679,525 | G→T | A1074D (GCT→GAT) | yphG ← | DUF4380 domain‑containing TPR repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,679,525 | 0 | G | T | 96.3% | 84.4 / ‑5.6 | 27 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (15/11); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG > NC_000913/2679460‑2679588 | gggTTTAGTGAACGCGGTTAAAAATTAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc > 2:452551/1‑68 (MQ=255) ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCg > 2:227250/1‑68 (MQ=255) gTTTAGTGAACGCGGTTAAAAATAAAAGGCTTCACGGTGGTGAATAATGCCGCTTTCGGCGAATCCgg > 2:337666/1‑68 (MQ=255) gagAACGCGGTTAAAAATAAAAGGCAACACGGTGGTGAATACTGACGCTTTCGGCCAATCAGGATTaa > 1:290125/3‑68 (MQ=255) gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTaa > 2:237699/1‑68 (MQ=255) tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt > 2:246725/1‑68 (MQ=255) gcgTTTAAAAATAATAGGCATCACGTTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg < 2:399230/68‑1 (MQ=255) gcgGTTAAAAATACAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg < 2:598359/68‑1 (MQ=255) gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg < 1:54104/68‑1 (MQ=255) agtcATCACGGTTGTGAATATTGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCATCGCGAg < 1:126791/65‑1 (MQ=255) ggCATCACGGTGGTGAATAATGACGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGc > 2:684332/1‑68 (MQ=255) gCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCa > 2:717784/1‑68 (MQ=255) agcACGGTGGCGAATAATGCCGCTCTCGGCCAATCCGGATTACTCGCCGTCAATTCCGCGCGAGCAGa < 1:655715/66‑1 (MQ=255) aTCACGGTTTTTAATAATTCAGCTTTCGGCCAAGTCGTATTAATCGCCGTCAATTCAGCGCGTGCAGa < 1:266307/68‑1 (MQ=255) aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa < 2:248708/68‑1 (MQ=255) aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa < 1:327818/68‑1 (MQ=255) aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa < 1:266309/68‑1 (MQ=255) aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa < 2:800472/68‑1 (MQ=255) cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt < 1:452551/68‑1 (MQ=255) ggAATAATGCCGCTTTCTGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc < 2:228059/67‑1 (MQ=255) ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa > 2:466192/1‑68 (MQ=255) aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCagc > 2:652276/1‑66 (MQ=255) aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc > 2:750796/1‑68 (MQ=255) gacGCTTTCGGCCAATCAGGATTAATAGCAGTCAAATCAGAGAGAGCAGACTCTTAATGCGAAGCCTc > 2:278314/3‑68 (MQ=255) gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc > 2:355170/1‑68 (MQ=255) gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc > 2:170273/1‑68 (MQ=255) ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcg > 1:352918/1‑68 (MQ=255) | GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG > NC_000913/2679460‑2679588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |