| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,811,035 | C→T | T89M (ACG→ATG) | mprA → | transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,811,035 | 0 | C | T | 95.5% | 60.2 / ‑4.1 | 22 | T89M (ACG→ATG) | mprA | transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (13/8); minor base A (1/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
TCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGA > NC_000913/2810985‑2811095 | tCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAAc > 1:311272/1‑68 (MQ=255) cAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACt > 1:372725/1‑68 (MQ=255) gCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTgg > 2:420901/1‑68 (MQ=255) tCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGAAGCGTATTGCCGATGAACTGGaaaa > 2:564789/1‑68 (MQ=255) aTTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAAcgcg > 2:661067/1‑68 (MQ=255) aTTAAGTTGAGCTCGTGTATCATCCAGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAAcgcg < 1:719672/68‑1 (MQ=255) tAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGt < 1:125688/68‑1 (MQ=255) gTTGTGCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTgg > 2:401704/1‑68 (MQ=255) tgtgCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGcc < 1:240087/42‑1 (MQ=255) tgtgCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGcc > 2:240087/1‑42 (MQ=255) tgtgCTCTTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGGTGGAt < 1:188448/68‑1 (MQ=255) tctTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAAc > 1:630902/1‑68 (MQ=255) ctTGGATCATCCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAAcg < 2:505038/68‑1 (MQ=255) atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGCTCGAACGTCGTg > 2:109837/1‑68 (MQ=255) atcatcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTg > 1:418411/1‑68 (MQ=255) atcCCGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGaaa < 1:420901/68‑1 (MQ=255) cccGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGc < 1:661067/68‑1 (MQ=255) cccGTACCAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGc > 1:23075/1‑68 (MQ=255) ccAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAACGCGATAAc > 1:382796/1‑68 (MQ=255) ccAACGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATacc > 1:33310/1‑66 (MQ=255) caaaCGCGATGCGTATTGCCGATGAACAGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATGAc > 1:116912/3‑68 (MQ=255) aaCGCGATGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGa < 1:496480/68‑1 (MQ=255) | TCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGA > NC_000913/2810985‑2811095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |