Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	44.5 / NA	13	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/9);  total (4/9)

AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATC  >  NC_000913/3413508‑3413626                                                                |                                                        aaaaTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATaa                                                     >  2:654023/1‑68 (MQ=255)                  tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCGTAAATCGTTAAATAaataat                                    <  2:289112/68‑1 (MQ=255)                  tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                    <  1:16393/68‑1 (MQ=255)                   cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                   <  1:697318/68‑1 (MQ=255)                   cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                   >  2:359803/1‑68 (MQ=255)                    tATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatat                                  <  1:200105/68‑1 (MQ=255)                         ccTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                       <  1:699706/58‑1 (MQ=255)                         ccTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                       >  2:699707/1‑58 (MQ=255)                               gtgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                       <  1:612977/68‑1 (MQ=255)                               gtgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                       <  2:228384/68‑1 (MQ=255)                                        taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc              >  2:805905/1‑68 (MQ=255)                                               tgCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACt       <  2:238083/67‑1 (MQ=255)                                                    atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc  <  2:288598/68‑1 (MQ=255)                                                                |                                                        AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATC  >  NC_000913/3413508‑3413626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑