Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,215	Δ1 bp	coding (446/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,215	0	A	.	100.0%	64.4 / NA	15	coding (446/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/5);  total (10/5)

ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276                                                            |                                                              accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                      >  1:2590/1‑68 (MQ=255)   caccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGc                                                    >  1:239323/1‑68 (MQ=255)   caccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGc                                                    >  1:872762/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        >  1:816985/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        <  2:608474/68‑1 (MQ=255)                 tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                      >  1:631632/1‑68 (MQ=255)                 tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                      >  2:522401/1‑68 (MQ=255)                   cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAtt                                    >  2:93447/1‑68 (MQ=255)                    ctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTg                                   >  2:911517/1‑68 (MQ=255)                        aTCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGctct                               <  2:940548/68‑1 (MQ=255)                              tctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtc                         >  2:681470/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  1:696503/1‑68 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  2:386351/68‑1 (MQ=255)                                                  acaGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGAc     <  2:803587/68‑1 (MQ=255)                                                     gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  2:944026/68‑1 (MQ=255)                                                            |                                                              ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑