| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 34,508 | 0 | G | C | 34.4% | 56.2 / 16.9 | 32 | G70A (GGA→GCA) | caiF | cai operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (16/5); new base C (0/11); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 | atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:516567/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:124069/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:733993/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:473486/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:299765/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:281156/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:832761/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTTAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 1:927915/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 1:33988/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 1:634411/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCAt < 1:439699/68‑1 (MQ=255) tCTGTCGGAAGTCACAGAAAGAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 2:155588/1‑68 (MQ=255) aaaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTg < 2:296600/68‑1 (MQ=255) aaTATGCTGCTATGTTAAGATTATCCCAAATATGCTGTAAGGGCGGGCATGCCAGTGTCAGCGACTgg < 2:281158/68‑1 (MQ=255) aaTATGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTgg < 1:117794/68‑1 (MQ=255) aaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg < 1:372003/68‑1 (MQ=255) aaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTgg < 2:507673/68‑1 (MQ=255) aaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTgg < 1:124069/68‑1 (MQ=255) tGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGTGGATGCCAGTGTCAGCGACTGGTTAAAGt < 2:288564/68‑1 (MQ=255) cGAAGTTATGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTgg < 2:765679/68‑1 (MQ=255) gAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGt > 2:37556/1‑68 (MQ=255) gatgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg > 2:519584/1‑50 (MQ=255) gatgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg < 1:519581/50‑1 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:762833/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:329942/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:399254/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:794839/1‑68 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAAttt > 1:698492/1‑68 (MQ=255) aGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAc > 2:190933/1‑68 (MQ=255) gcaggAAGGGCGGGGATGCCAGTGTCAGAGACTGGATAAAGTGGTCGATATCGATGAGCAAATTTAcg > 2:867946/4‑68 (MQ=255) gCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAcg > 2:678414/1‑68 (MQ=255) cTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAcgc > 1:296000/1‑68 (MQ=255) ggCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTg > 1:211001/1‑68 (MQ=255) cGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 1:190933/68‑1 (MQ=255) cGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:539128/68‑1 (MQ=255) cGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 1:516564/68‑1 (MQ=255) cGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGGCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:786598/68‑1 (MQ=255) cGGGCATGCCAGCGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:787890/68‑1 (MQ=255) cGGGCACGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:265779/68‑1 (MQ=255) gtgATGCCAGTGTCAGCGACTTGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCa < 1:867942/66‑1 (MQ=255) | ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |